产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸化FAK(Tyr397)重组兔单克隆抗体(31H5L17)
目录 :
700255
规格 :
100微克
价格 :
美国375.00
克隆性 :
重组
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
31H5L17
反应物种 :
人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 35
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 图 6a
Mondal B, Jin H, Kallappagoudar S, Sedkov Y, Martinez T, Sentmanat M, et al. The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. elife. 2020;9: pubmed 出版商
  • 免疫组化-冰冻切片; 人类; 1:100; 图 7s2b
  • 免疫组化-石蜡切片; 人类; 1:100; 图 3a, 7s2a
  • 免疫印迹; 人类; 1:1000; 图 4b, 6s1c
  • 免疫印迹; 小鼠; 1:1000; 图 2c
Diaz Osterman C, Ozmadenci D, Kleinschmidt E, Taylor K, Barrie A, Jiang S, et al. FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy. elife. 2019;8: pubmed 出版商
  • 免疫细胞化学; 人类; 1:100; 图 s4b
Lee J, Chang J, Dominguez A, Lee H, Nam S, Chang J, et al. YAP-independent mechanotransduction drives breast cancer progression. Nat Commun. 2019;10:1848 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 6d
Geng Y, Liu X, Liang J, Habiel D, Kulur V, Coelho A, et al. PD-L1 on invasive fibroblasts drives fibrosis in a humanized model of idiopathic pulmonary fibrosis. JCI Insight. 2019;4: pubmed 出版商
  • 免疫印迹; 犬; 1:1000; 图 7b
Van Itallie C, Tietgens A, Aponte A, Gucek M, Cartagena Rivera A, Chadwick R, et al. MARCKS-related protein regulates cytoskeletal organization at cell-cell and cell-substrate contacts in epithelial cells. J Cell Sci. 2018;131: pubmed 出版商
  • 免疫印迹; 人类; 图 5
Oblinger J, Burns S, Huang J, Pan L, Ren Y, Shen R, et al. Overexpression of eIF4F components in meningiomas and suppression of meningioma cell growth by inhibiting translation initiation. Exp Neurol. 2018;299:299-307 pubmed 出版商
  • 免疫印迹; 人类; 图 7d
Lin Y, Zhen Y, Chien K, Lee I, Lin W, Chen M, et al. LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration. Mol Biol Cell. 2017;28:1054-1065 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 图 3c
  • 免疫印迹; 小鼠; 图 3e
Goreczny G, Ouderkirk Pecone J, Olson E, Krendel M, Turner C. Hic-5 remodeling of the stromal matrix promotes breast tumor progression. Oncogene. 2017;36:2693-2703 pubmed 出版商
  • 免疫印迹; 人类; 图 2c
Chien J, Tsen S, Chien C, Liu H, Tung C, Lin C. ?TAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov. 2016;2:16006 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠; 图 5
Budina Kolomets A, Webster M, Leu J, Jennis M, Krepler C, Guerrini A, et al. HSP70 Inhibition Limits FAK-Dependent Invasion and Enhances the Response to Melanoma Treatment with BRAF Inhibitors. Cancer Res. 2016;76:2720-30 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100; 图 1
  • 免疫印迹; 人类; 1:100; 图 3
Ren K, Lu X, Yao N, Chen Y, Yang A, Chen H, et al. Focal adhesion kinase overexpression and its impact on human osteosarcoma. Oncotarget. 2015;6:31085-103 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠; 1:100
Kaku M, Rosales Rocabado J, Kitami M, Ida T, Akiba Y, Yamauchi M, et al. Mechanical Loading Stimulates Expression of Collagen Cross-Linking Associated Enzymes in Periodontal Ligament. J Cell Physiol. 2016;231:926-33 pubmed 出版商
  • 免疫细胞化学; 大鼠; 图 5c
Jiang J, Zhang Z, Yuan X, Poo M. Spatiotemporal dynamics of traction forces show three contraction centers in migratory neurons. J Cell Biol. 2015;209:759-74 pubmed 出版商
  • 免疫印迹; 人类; 图 3, 4
Puig M, Lugo R, Gabasa M, Giménez A, Velásquez A, Galgoczy R, et al. Matrix stiffening and β1 integrin drive subtype-specific fibroblast accumulation in lung cancer. Mol Cancer Res. 2015;13:161-73 pubmed 出版商
Aye J, Stafman L, Williams A, Garner E, Stewart J, Anderson J, et al. The effects of focal adhesion kinase and platelet-derived growth factor receptor beta inhibition in a patient-derived xenograft model of primary and metastatic Wilms tumor. Oncotarget. 2019;10:5534-5548 pubmed 出版商
Shannon M, Pineau J, Griffié J, Aaron J, Peel T, Williamson D, et al. Differential nanoscale organisation of LFA-1 modulates T-cell migration. J Cell Sci. 2019;133: pubmed 出版商
Garg A, Jones T, Moss S, Mishra S, Kaul K, Ahirwar D, et al. Electromagnetic fields alter the motility of metastatic breast cancer cells. Commun Biol. 2019;2:303 pubmed 出版商
Stowers R, Shcherbina A, Israeli J, Gruber J, Chang J, Nam S, et al. Matrix stiffness induces a tumorigenic phenotype in mammary epithelium through changes in chromatin accessibility. Nat Biomed Eng. 2019;3:1009-1019 pubmed 出版商
Palamà I, D Amone S, Ratano P, Donatelli A, Liscio A, Antonacci G, et al. Mechanical Durotactic Environment Enhances Specific Glioblastoma Cell Responses. Cancers (Basel). 2019;11: pubmed 出版商
Kim T, Lei L, Seong J, Suh J, Jang Y, Jung S, et al. Matrix Rigidity-Dependent Regulation of Ca2+ at Plasma Membrane Microdomains by FAK Visualized by Fluorescence Resonance Energy Transfer. Adv Sci (Weinh). 2019;6:1801290 pubmed 出版商
Liu S, Hsu T, Chang Y, Chung A, Jiang S, OuYang C, et al. Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling. Nat Commun. 2018;9:5105 pubmed 出版商
Haage A, Goodwin K, Whitewood A, Camp D, Bogutz A, Turner C, et al. Talin Autoinhibition Regulates Cell-ECM Adhesion Dynamics and Wound Healing In Vivo. Cell Rep. 2018;25:2401-2416.e5 pubmed 出版商
Wu W, Liao Y. Downregulation of C-Terminal Tensin-Like Protein (CTEN) Suppresses Prostate Cell Proliferation and Contributes to Acinar Morphogenesis. Int J Mol Sci. 2018;19: pubmed 出版商
Topal T, Hong X, Xue X, Fan Z, Kanetkar N, Nguyen J, et al. Acoustic Tweezing Cytometry Induces Rapid Initiation of Human Embryonic Stem Cell Differentiation. Sci Rep. 2018;8:12977 pubmed 出版商
Gulvady A, Dubois F, Deakin N, Goreczny G, Turner C. Hic-5 expression is a major indicator of cancer cell morphology, migration, and plasticity in three-dimensional matrices. Mol Biol Cell. 2018;29:1704-1717 pubmed 出版商
Ho L, Skiba N, Ullmer C, Rao P. Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci. 2018;59:1969-1984 pubmed 出版商
Millar M, Corson N, Xu L. The Adhesion G-Protein-Coupled Receptor, GPR56/ADGRG1, Inhibits Cell-Extracellular Matrix Signaling to Prevent Metastatic Melanoma Growth. Front Oncol. 2018;8:8 pubmed 出版商
Sun S, Wu H, Guan J. Nuclear FAK and its kinase activity regulate VEGFR2 transcription in angiogenesis of adult mice. Sci Rep. 2018;8:2550 pubmed 出版商
Chang T, Chen C, Lee M, Chang Y, Lu C, Lu S, et al. Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery. elife. 2017;6: pubmed 出版商
Gabasa M, Duch P, Jorba I, Giménez A, Lugo R, Pavelescu I, et al. Epithelial contribution to the profibrotic stiff microenvironment and myofibroblast population in lung fibrosis. Mol Biol Cell. 2017;28:3741-3755 pubmed 出版商
Kuenzi B, Remsing Rix L, Stewart P, Fang B, Kinose F, Bryant A, et al. Polypharmacology-based ceritinib repurposing using integrated functional proteomics. Nat Chem Biol. 2017;13:1222-1231 pubmed 出版商
Cavazzoni A, La Monica S, Alfieri R, Ravelli A, Van Der Steen N, Sciarrillo R, et al. Enhanced efficacy of AKT and FAK kinase combined inhibition in squamous cell lung carcinomas with stable reduction in PTEN. Oncotarget. 2017;8:53068-53083 pubmed 出版商
Rodriguez D, Braden B, Boyer S, Taketa D, Setar L, Calhoun C, et al. In vivo manipulation of the extracellular matrix induces vascular regression in a basal chordate. Mol Biol Cell. 2017;28:1883-1893 pubmed 出版商
Swaminathan V, Fischer R, Waterman C. The FAK-Arp2/3 interaction promotes leading edge advance and haptosensing by coupling nascent adhesions to lamellipodia actin. Mol Biol Cell. 2016;27:1085-100 pubmed 出版商
Zaman G, Sunters A, Galea G, Javaheri B, Saxon L, Moustafa A, et al. Loading-related regulation of transcription factor EGR2/Krox-20 in bone cells is ERK1/2 protein-mediated and prostaglandin, Wnt signaling pathway-, and insulin-like growth factor-I axis-dependent. J Biol Chem. 2012;287:3946-62 pubmed 出版商
产品信息
产品类型 :
抗体
产品名称 :
磷酸化FAK(Tyr397)重组兔单克隆抗体(31H5L17)
目录# :
700255
规格 :
100微克
价格 :
美国375.00
克隆性 :
重组单克隆
纯度 :
蛋白质A
宿主 :
兔
反应物种 :
人类, 小鼠
应用 :
ELISA: 1:500-1:2,000, Immunocytochemistry: 1:500-1:2,000, Immunohistochemistry (Paraffin): 1:10-1:1,000, Western Blot: 1:500-1:2,000
物种 :
人类, 小鼠
克隆 :
31H5L17
抗体亚型 :
IgG
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
免疫原 :
A peptide corresponding to amino acids 390-401 of Q05397.
格式 :
液体
应用w/稀释 :
ELISA: 1:500-1:2,000, Immunocytochemistry: 1:500-1:2,000, Immunohistochemistry (Paraffin): 1:10-1:1,000, Western Blot: 1:500-1:2,000
别名 :
CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国