α微管蛋白单克隆抗体(236-10501)
赛默飞世尔
目录: A11126
小鼠 单克隆 (236-10501)
反应物种: African green monkey, Cyrtanthus mackenii, Leishmania , 仓鼠, field poppy, malaria parasite P. falciparum, kangaroo, 人类, 小鼠, 大鼠, , fruit fly , 非洲爪蛙
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 129
规格: 50微克
价格: 美国346
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α微管蛋白单克隆抗体(DM1A)
赛默飞世尔
目录: 62204
小鼠 单克隆 (DM1A)
反应物种: African green monkey, 婴儿利什曼原虫, 淡水涡虫;真涡虫, 豚鼠, 仓鼠, 人类, 小鼠, 大鼠, , , , pigs , fruit fly , 家羊, 非洲爪蛙
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 其他
文章摘录数: 153
规格: 40微升
价格: 美国237
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小鼠 单克隆 (236-10501)
反应物种: 人类, 小鼠,
共轭标签: 生物素
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 4
规格: 50微克
价格: 美国397
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α微管蛋白多克隆抗体
赛默飞世尔
目录: PA5-19489
domestic rabbit 多克隆
反应物种: 中国人仓鼠, 人类, 小鼠, 大鼠, , , fruit fly
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
文章摘录数: 6

Immunoprecipitation of acetylated alpha-Tubulin was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an alpha-Tubulin polyclonal antibody (Product # PA5-19489) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

Western blot analysis of HeLa Whole Cell Lysate using PA5-19489, alpha Tubulin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19489 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19489 staining of A431 Whole Cell Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Immunofluorescent staining of HeLa cells using PA5-19489, anti-Alpha Tubulin antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
规格: 50微克
价格: 美国411.00
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