Nanog Monoclonal Antibody (hNanog.2), eBioscience
赛默飞世尔
目录: 14-5768-80
小鼠 单克隆 (hNanog.2)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 14
规格: 25微克
价格: 美国309.00
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Nanog Monoclonal Antibody (hNanog.2), eBioscience
赛默飞世尔
目录: 14-5768-82
小鼠 单克隆 (hNanog.2)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 15
规格: 100微克
价格: 美国309.00
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Nanog Polyclonal Antibody
赛默飞世尔
目录: PA1-097
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 15

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows nuclear localization in the human iPS cell line HEL 11.4. Nanog staining (red) and an overlay image of Nanog with DAPI (pink) is shown. HEL-11.4 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097) at a dilution of 1:100 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken on at 20X magnification.

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows nuclear localization in the human embryonic stem cell line H9. Nanog staining (red) and an overlay image of Nanog with DAPI (pink) is shown. H9 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097) at a dilution of 1:100 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken on at 20X magnification.

Western blot analysis of Nanog was performed by loading 80ug of NCCIT, NTERA-2 and negative control HeLa whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing Nanog (Product # PA1-097) at a dilution of 1:2000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34078).
规格: 100微克
价格: 美国470.00
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Nanog Polyclonal Antibody
赛默飞世尔
目录: PA1-097X
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 14

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows specific expression in human embryonal carcinoma NTERA-2 cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-547 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of Nanog was performed by loading 80ug of NCCIT, NTERA-2 and negative control HeLa whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing Nanog (Product # PA1-097) at a dilution of 1:2000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34078).

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows nuclear localization in the human embryonic stem cell line H9. Nanog staining (red) and an overlay image of Nanog with DAPI (pink) is shown. H9 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097) at a dilution of 1:100 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken on at 20X magnification.
规格: 20微升
价格: 美国179.00
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Nanog Monoclonal Antibody (23D2-3C6)
赛默飞世尔
目录: MA1-017
小鼠 单克隆 (23D2-3C6)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
文章摘录数: 9
规格: 100微克
价格: 美国400.00
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Nanog Polyclonal Antibody, PeproTech
赛默飞世尔
目录: 500-P236-100UG
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
文章摘录数: 6
规格: 100微克
价格: 美国270.00
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Nanog Polyclonal Antibody, PeproTech
赛默飞世尔
目录: 500-P236-1MG
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
文章摘录数: 6
规格: 1毫克
价格: 美国1836.00
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Nanog Polyclonal Antibody, PeproTech
赛默飞世尔
目录: 500-P236-50UG
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
文章摘录数: 6
规格: 50微克
价格: 美国211.00
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Nanog Polyclonal Antibody
赛默飞世尔
目录: PA5-18406
domestic goat 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
文章摘录数: 2

Immunofluorescent staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes using PA5-18406 at a concentration of 5ug/ml.

Western Blot staining of Human Ovary lysate using PA5-18406 at a concentration of 0.03 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
规格: 100微克
价格: 美国456.00
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Nanog Polyclonal Antibody
赛默飞世尔
目录: PA5-20889
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
文章摘录数: 3

Immunohistochemical staining of human spleen tissue using PA5-20889 anti-NANOG antibody at a concentration of 5 ug/mL.

Western blot analysis of human spleen tissue lysate using PA5-20889 anti-NANOG antibody at (A) 1 and (B) 2 ug/mL.
规格: 100微克
价格: 美国450.00
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Nanog Polyclonal Antibody
赛默飞世尔
目录: PA1-41577
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
文章摘录数: 1

规格: 100微克
价格: 美国456.00
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Nanog Monoclonal Antibody (1E6C4)
赛默飞世尔
目录: MA5-15469
小鼠 单克隆 (1E6C4)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
文章摘录数: 1

Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
规格: 100微升
价格: 美国520.00
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Nanog Polyclonal Antibody, PE
赛默飞世尔
目录: PA5-46891
domestic goat 多克隆
反应物种: 人类
共轭标签: PE
应用: 流式细胞仪
文章摘录数: 1
规格: 100试验
价格: 美国374.00
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Nanog Polyclonal Antibody
赛默飞世尔
目录: PA5-85110
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 1
规格: 100微升
价格: 美国446.00
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Phospho-Nanog (Ser71) Polyclonal Antibody
赛默飞世尔
目录: PA5-13078
domestic rabbit 多克隆
反应物种: 人类
修饰: 磷酸化
应用: 免疫细胞化学, dot blot

规格: 400微升
价格: 美国446.00
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Nanog Polyclonal Antibody
赛默飞世尔
目录: PA5-18678
domestic goat 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片

Immunohistochemical staining of paraffin embedded of Human Testis using PA5-18678 at a concentration of 2 ug/ml. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.

Western Blot staining of nuclear HeLa cell lysate using PA5-18678 at a concentration of 0.05 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
规格: 100微克
价格: 美国446.00
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Phospho-Nanog (Ser285) Polyclonal Antibody
赛默飞世尔
目录: PA5-13079
domestic rabbit 多克隆
反应物种: 人类
修饰: 磷酸化
应用: dot blot
规格: 400微升
价格: 美国446.00
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Nanog Monoclonal Antibody (23D2-3C6), DyLight™ 488
赛默飞世尔
目录: MA1-017-D488
小鼠 单克隆 (23D2-3C6)
反应物种: 人类
共轭标签: DyLight488
应用: 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 美国390.00
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Nanog Monoclonal Antibody (23D2-3C6), DyLight™ 550
赛默飞世尔
目录: MA1-017-D550
小鼠 单克隆 (23D2-3C6)
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 酶联免疫吸附测定, 免疫细胞化学
规格: 100微升
价格: 美国380.00
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Nanog Monoclonal Antibody (23D2-3C6), DyLight™ 650
赛默飞世尔
目录: MA1-017-D650
小鼠 单克隆 (23D2-3C6)
反应物种: 人类
共轭标签: DyLight系列染料650
应用: 免疫细胞化学
规格: 100微升
价格: 美国420.00
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Nanog Monoclonal Antibody (23D2-3C6), HRP
赛默飞世尔
目录: MA1-017-HRP
小鼠 单克隆 (23D2-3C6)
反应物种: 人类
共轭标签: HRP
应用: 免疫印迹
规格: 100微升
价格: 美国400.00
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Nanog Monoclonal Antibody (GT3312)
赛默飞世尔
目录: MA5-31459
小鼠 单克隆 (GT3312)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 美国436.00
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Nanog Recombinant Rabbit Monoclonal Antibody (SC05-70)
赛默飞世尔
目录: MA5-32198
domestic rabbit 重组 (SC05-70)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 美国446.00
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Nanog Polyclonal Antibody, Biotin, PeproTech
赛默飞世尔
目录: 500-P236BT-1MG
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定
规格: 2 x 500微克
价格: 美国3672.00
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Nanog Polyclonal Antibody, Biotin, PeproTech
赛默飞世尔
目录: 500-P236BT-25UG
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定
规格: 25微克
价格: 美国211.00
至产商
Nanog Polyclonal Antibody, Biotin, PeproTech
赛默飞世尔
目录: 500-P236BT-50UG
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定
规格: 50微克
价格: 美国270.00
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Nanog Recombinant Rabbit Monoclonal Antibody (2G6)
赛默飞世尔
目录: MA5-50178
domestic rabbit 重组 (2G6)
反应物种: 人类
应用: 酶联免疫吸附测定, 流式细胞仪
规格: 100微升
价格: 美国389.00
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