O链接N乙酰葡糖胺(O-GlcNAc)单克隆抗体(RL2)
赛默飞世尔
目录: MA1-072
小鼠 单克隆 (RL2)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀 , 免疫组化-石蜡切片, dot blot, 其他, 免疫印迹基因敲除验证
文章摘录数: 163
规格: 100微升
价格: 美国450.00
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O链接N乙酰葡糖胺(O-GlcNAc)单克隆抗体(HGAC85)
赛默飞世尔
目录: MA1-076
小鼠 单克隆 (HGAC85)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀
文章摘录数: 21
规格: 100微克
价格: 美国450.00
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O链接N乙酰葡糖胺(O-GlcNAc)单克隆抗体(18B10.C7)
赛默飞世尔
目录: MA1-038
小鼠 单克隆 (18B10.C7)
反应物种: 人类, 犬
应用: 免疫印迹, 免疫沉淀
文章摘录数: 4

Immunoprecipitation of Glycogen Synthase was performed on HepG2 cells, glucose and serum starved for 36 hours. The antigen:antibody complex was formed by binding 500µg whole cell lysate with 5µg of mouse monoclonal antibody recognizing O-GlcNAc (Product # MA1-038) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20463). The beads were washed to remove non-bound material and a low-pH elution buffer was used to dissociate bound antigen from the antibody-bound bead. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Glycogen Synthase (Product # PA5-17459) at a dilution of 1:1000 overnight rotating at 4°C. Membranes were then washed in TBST and probed with Clean-blot IP detection reagent (Product #21230) at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34087). The image is a Western blot with antibody PA5-17459 showing enrichment of glycogen synthase by O-GlcNAc precipitation. Lane 1 indicates 25 ug of HepG2 whole cell lysate input; Lane 2 indicates the IP elution.

Western blot analysis of O-GlcNAc was performed by loading 10µg of HEK293T or C2C12 whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing O-GlcNAc (Product #MA1-038) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075). The image is a Western blot with antibody MA1-038 with untreated (lanes 1 and 3) versus treated (lanes 2 and 4) for O-GlcNAc enrichment.
规格: 100微克
价格: 美国430.00
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OGT多克隆抗体
赛默飞世尔
目录: PA5-22071
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 2

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft tissue using PA5-22071 anti-O-GlcNAc transferase antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using PA5-22071 anti-O-GlcNAc transferase antibody at 1:200 dilution.
规格: 100微升
价格: 美国450.00
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OGT多克隆抗体
赛默飞世尔
目录: PA5-13654
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
文章摘录数: 1

规格: 400微升
价格: 美国456.00
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O链接N乙酰葡糖胺(O-GlcNAc)单克隆抗体(9D1.E4)
赛默飞世尔
目录: MA1-039
小鼠 单克隆 (9D1.E4)
反应物种: 人类
应用: 免疫印迹, 免疫沉淀
文章摘录数: 1

Western blot analysis of O-GlcNAc was performed by loading 10µg of HEK293T or C2C12 whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing O-GlcNAc (Product #MA1-039) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075). The image is a Western blot with antibody MA1-039 with untreated (lanes 1 and 3) versus treated (lanes 2 and 4) for O-GlcNAc enrichment.

Immunoprecipitation of Glycogen Synthase was performed on HepG2 cells, glucose and serum starved for 36 hours. The antigen:antibody complex was formed by binding 500µg whole cell lysate with 5µg of mouse monoclonal antibody recognizing O-GlcNAc (Product # MA1-039) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20463). The beads were washed to remove non-bound material and a low-pH elution buffer was used to dissociate bound antigen from the antibody-bound bead. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Glycogen Synthase (Product # PA5-17459) at a dilution of 1:1000 overnight rotating at 4°C. Membranes were then washed in TBST and probed with Clean-blot IP detection reagent (Product #21230) at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34087). The image is a Western blot with antibody PA5-17459 showing enrichment of glycogen synthase by O-GlcNAc precipitation. Lane 1 indicates 25 ug of HepG2 whole cell lysate input; Lane 2 indicates the IP elution.
规格: 100微克
价格: 美国450.00
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OGT多克隆抗体
赛默飞世尔
目录: PA5-83154
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
文章摘录数: 1
规格: 100微升
价格: 美国456.00
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OGT多克隆抗体
赛默飞世尔
目录: PA5-18658
domestic goat 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western Blot staining of Rat Pancreas lysate using PA5-18658 at a concentration of 0.05 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
规格: 100微克
价格: 美国436.00
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OGT多克隆抗体
赛默飞世尔
目录: PA5-22092
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
规格: 100微升
价格: 美国446.00
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O-GlcNAc Monoclonal Antibody (RL2), PE-Cyanine7, eBioscience
赛默飞世尔
目录: 25-9793-42
小鼠 单克隆 (RL2)
反应物种: 人类
共轭标签: PE/Cy7
应用: 流式细胞仪
规格: 100试验
价格: 美国339.00
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O-GlcNAc Monoclonal Antibody (RL2), PE, eBioscience
赛默飞世尔
目录: 12-9793-42
小鼠 单克隆 (RL2)
反应物种: 人类
共轭标签: PE
应用: 流式细胞仪
规格: 100试验
价格: 美国339.00
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O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor™ 647, eBioscience
赛默飞世尔
目录: 51-9793-42
小鼠 单克隆 (RL2)
反应物种: 人类
共轭标签: AF647
应用: 流式细胞仪
规格: 100试验
价格: 美国339.00
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O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor™ 488, eBioscience
赛默飞世尔
目录: 53-9793-42
小鼠 单克隆 (RL2)
反应物种: 人类
共轭标签: AF488
应用: 流式细胞仪
规格: 100试验
价格: 美国339.00
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O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor™ 488, eBioscience
赛默飞世尔
目录: 53-9793-41
小鼠 单克隆 (RL2)
反应物种: 人类
共轭标签: AF488
应用: 流式细胞仪
规格: 25试验
价格: 美国149.00
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OGT多克隆抗体
赛默飞世尔
目录: PA5-83155
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
规格: 100微升
价格: 美国456.00
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OGT多克隆抗体
赛默飞世尔
目录: PA5-95319
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100微克
价格: 美国446.00
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OGT多克隆抗体
赛默飞世尔
目录: PA5-96026
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国446.00
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OGT重组兔单克隆抗体(JB44-39)
赛默飞世尔
目录: MA5-34661
domestic rabbit 重组 (JB44-39)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100微升
价格: 美国446.00
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OGT重组兔单克隆抗体(ARC0790)
赛默飞世尔
目录: MA5-35376
domestic rabbit 重组 (ARC0790)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫沉淀
规格: 100微升
价格: 美国446.00
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OGT重组兔单克隆抗体(HL1641)
赛默飞世尔
目录: MA5-47117
domestic rabbit 重组 (HL1641)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国416.00
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OGT重组兔单克隆抗体(HL1642)
赛默飞世尔
目录: MA5-47118
domestic rabbit 重组 (HL1642)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国416.00
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OGT重组兔单克隆抗体(8G7)
赛默飞世尔
目录: MA5-49895
domestic rabbit 重组 (8G7)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片
规格: 100微升
价格: 美国389.00
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