产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
肿瘤坏死因子甲单克隆抗体(2TNF-H33), 生物素
目录 :
M302B
规格 :
250微克
价格 :
美国428
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
生物素
克隆名称 :
2TNF-H33
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
文章摘录数: 19
| 出版应用/物种/样本/稀释 | 参考文献 |
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| López Antón N, Hermann C, Murillo R, Merfort I, Wanner G, Vollmar A, et al. Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses. Apoptosis. 2007;12:141-53 pubmed
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| Urbanowska T, Mangialaio S, Zickler C, Cheevapruk S, Hasler P, Regenass S, et al. Protein microarray platform for the multiplex analysis of biomarkers in human sera. J Immunol Methods. 2006;316:1-7 pubmed
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| Pedraza Sánchez S, Gonzalez Hernandez Y, Escobar Gutierrez A, Ramachandra L. The immunostimulant RU41740 from Klebsiella pneumoniae activates human cells in whole blood to potentially stimulate innate and adaptive immune responses. Int Immunopharmacol. 2006;6:635-46 pubmed
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Pedraza Sánchez S, Hise A, Ramachandra L, Arechavaleta Velasco F, King C. Reduced frequency of a CD14+ CD16+ monocyte subset with high Toll-like receptor 4 expression in cord blood compared to adult blood contributes to lipopolysaccharide hyporesponsiveness in newborns. Clin Vaccine Immunol. 2013;20:962-71 pubmed 出版商
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Greig N, Giordano T, Zhu X, Yu Q, Perry T, Holloway H, et al. Thalidomide-based TNF-alpha inhibitors for neurodegenerative diseases. Acta Neurobiol Exp (Wars). 2004;64:1-9 pubmed
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Tiberio L, Fletcher L, Eldridge J, Duncan D. Host factors impacting the innate response in humans to the candidate adjuvants RC529 and monophosphoryl lipid A. Vaccine. 2004;22:1515-23 pubmed
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Schotte H, Schluter B, Willeke P, Mickholz E, Schorat M, Domschke W, et al. Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis. Rheumatology (Oxford). 2004;43:960-4 pubmed
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Zhu X, Giordano T, Yu Q, Holloway H, Perry T, Lahiri D, et al. Thiothalidomides: novel isosteric analogues of thalidomide with enhanced TNF-alpha inhibitory activity. J Med Chem. 2003;46:5222-9 pubmed
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Solomon S, Masilamani M, Mohanty S, Schwab J, Boneberg E, Illges H. Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients. Rheumatol Int. 2004;24:71-6 pubmed
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Loffreda S, Muratori P, Muratori L, Mele L, Bianchi F, Lenzi M. Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients. J Hepatol. 2003;38:230-6 pubmed
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Montenegro S, Abath F, Domingues A, Melo W, Morais C, Coutinho E, et al. Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis. Infect Immun. 2002;70:5903-12 pubmed
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Shi Y, Lutz C. Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells. Viral Immunol. 2002;15:213-25 pubmed
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Libraty D, Pichyangkul S, Ajariyakhajorn C, Endy T, Ennis F. Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis. J Virol. 2001;75:3501-8 pubmed
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Eigler A, Matschke V, Hartmann G, Erhardt S, Boyle D, Firestein G, et al. Suppression of TNF-alpha production in human mononuclear cells by an adenosine kinase inhibitor. J Leukoc Biol. 2000;68:97-103 pubmed
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Marth T, Kelsall B. Regulation of interleukin-12 by complement receptor 3 signaling. J Exp Med. 1997;185:1987-95 pubmed
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图像
图像 1 :
Sandwich ELISA analysis of Human TNF alpha Biotinylated MAb (Pierce product # M302B) was performed using components of Thermo Fisher Scientific Human TNF alpha Colorimetric ELISA Kit (Pierce product # EH3TNFA) by loading 50ul per well of Recombinant Human TNF alpha in octuplicate at 1000, 500, 250, 125, 62.5, 31.2, 15.6, and 0 pg/ml across a 5µg/ml rabbit anti-Human TNF pre-coated plate and incubated for 1 hour at room temperature. The plate was washed then incubated with 100 µl per well of Human TNF alpha Biotinylated MAb (M302B) in duplicate at 2, 1, 0.5, and 0.25 µg/ml for 1 hour at room temperature. The plate was washed and incubated with 100 µl per well of Ultra Streptavidin-HRP (Pierce product # N504) in all test wells at 1:1,500 for 30 minutes at room temperature. The plate was washed then incubated with 100ul per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid and absorbance was read on a spectrophotometer at 450-550nm.
产品信息
产品类型 :
抗体
产品名称 :
肿瘤坏死因子甲单克隆抗体(2TNF-H33), 生物素
目录# :
M302B
规格 :
250微克
定价 :
美国428
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
人类
应用 :
ELISA: Assay-dependent, Western Blot: Assay-dependent
物种 :
人类
克隆 :
2TNF-H33
抗体亚型 :
IgG1, kappa
储存 :
-20°C
描述 :
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
免疫原 :
重组人类肿瘤坏死因子甲
格式 :
液体
应用w/稀释 :
ELISA: Assay-dependent, Western Blot: Assay-dependent
别名 :
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
更多信息或购买 :
公司信息
赛默飞世尔