产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
AKT2单克隆抗体(4H7)
目录 :
MA1-034X
规格 :
20微升
价格 :
美国166
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
4H7
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
图像
图像 1 :
赛默飞世尔 MA1-034X 图像 1
Immunohistochemistry was performed on biopsies of deparaffinized normal human medulla oblongata and human esophageal cancer tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Akt-2 (Product # MA1-034) overnight at 4C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate cytoplasmic localization of Akt-2.
图像 2 :
赛默飞世尔 MA1-034X 图像 2
Immunoprecipitation of Akt-2 was performed on HeLa cells. The antigen:antibody complex was formed by incubating 750µg whole cell lysate with 2µg of mouse monoclonal antibody recognizing Akt-2 (Product # MA1-034) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20423) . Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Akt-2 (Product # MA1-034) at a dilution of 1:1000 overnight rotating at 4C. Membranes were washed in TBST and probed with Clean Blot IP Detection reagent (Product # 21230) at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
图像 3 :
赛默飞世尔 MA1-034X 图像 3
Western blot analysis of Akt-2 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing Akt-2 (Product # MA1-034) at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
产品信息
产品类型 :
抗体
产品名称 :
AKT2单克隆抗体(4H7)
目录# :
MA1-034X
规格 :
20微升
定价 :
美国166
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Human, Mouse, Non-human primate, Rat
应用 :
ChIP assay: 1-3 µL, Immunocytochemistry: 1:10-1:100, Immunohistochemistry: 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:1,000
物种 :
Human, Mouse, Non-human primate, Rat
克隆 :
4H7
抗体亚型 :
IgG1
储存 :
-20°C
描述 :
Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.
免疫原 :
Full length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.
格式 :
液体
应用w/稀释 :
ChIP assay: 1-3 µL, Immunocytochemistry: 1:10-1:100, Immunohistochemistry: 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:1,000
别名 :
2410016A19Rik; AKT serine/threonine kinase 2; Akt2; AKT2 kinase; AW554154; HIHGHH; murine thymoma viral (v-akt) homolog-2; murine thymoma viral (v-akt) oncogene homolog 2; PKB; PKB beta; PKBB; PKBBETA; PRKBB; protein kinase Akt-2; Protein kinase B beta; protein kinase B, beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-BETA; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; thymoma viral proto-oncogene 2; v-akt murine thymoma viral oncogene homolog 2
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国