产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
信号传导子及转录激活子1单克隆抗体(15H3)
目录 :
MA1-037X
规格 :
20微升
价格 :
美国160
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
15H3
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 4
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 1:1000; 图 3b
Weng Y, Shi Y, Xia X, Zhou W, Wang H, Wang C. A multi-shRNA vector enhances the silencing efficiency of exogenous and endogenous genes in human cells. Oncol Lett. 2017;13:1553-1562 pubmed 出版商
  • 免疫印迹; 人类; 图 3a
Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 4
Campia I, Buondonno I, Castella B, Rolando B, Kopecka J, Gazzano E, et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS ONE. 2015;10:e0126159 pubmed 出版商
Normand E, Franco A, Moreau A, Marcil V. Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts. Sci Rep. 2017;7:3173 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA1-037X 图像 1
Immunofluorescent analysis of STAT1 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a STAT1 monoclonal antibody (Product # MA1-037) at a dilution of 1:20 overnight at 4 ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). STAT1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
图像 2 :
赛默飞世尔 MA1-037X 图像 2
Western blot analysis of STAT1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing STAT1 (Product # MA1-037) at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1% Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
图像 3 :
赛默飞世尔 MA1-037X 图像 3
Immunoprecipitation of STAT1 was performed on U2OS cells. The antigen:antibody complex was formed by incubating 750µg whole cell lysate with 2µg of mouse monoclonal antibody recognizing STAT1 (Product # MA1-037) overnight on a rocking platform at 4C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20423) . Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing STAT1 (Product # MA1-037) at a dilution of 1:1000 overnight rotating at 4C. Membranes were washed in TBS-0.1% Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
产品信息
产品类型 :
抗体
产品名称 :
信号传导子及转录激活子1单克隆抗体(15H3)
目录# :
MA1-037X
规格 :
20微升
定价 :
美国160
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Human, Non-human primate
应用 :
Immunocytochemistry: 1:20, Immunoprecipitation: 2 µg, Western Blot: 1:1,000
物种 :
Human, Non-human primate
克隆 :
15H3
抗体亚型 :
IgG1
储存 :
-20°C
描述 :
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
免疫原 :
Protein expressed in 293T cell transfected with human STAT1 expression vector.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:20, Immunoprecipitation: 2 µg, Western Blot: 1:1,000
别名 :
2010005J02Rik; AA408197; CANDF7; DD6G4-4; DKFZp686B04100; IMD31A; IMD31B; IMD31C; ISGF-3; LOW QUALITY PROTEIN: signal transducer and activator of transcription 1-alpha/beta; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT1; STAT-1; stat1 alpha; STAT91; transcription factor ISGF-3; transcription factor ISGF-3 components p91/p84
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国