产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
PSD-95单克隆抗体(7E3-1B8)
目录 :
MA1-046
规格 :
100微升
价格 :
美国449.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
7E3-1B8
反应物种 :
African green monkey, 热带爪蟾, 人类, 小鼠, 大鼠, , 斑马鱼, domestic rabbit, roundworm
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 染色质免疫沉淀 , 免疫组化-冰冻切片, 免疫组化-自由浮动切片, 抑制或激活实验
更多信息或购买 :
文章摘录数: 265
出版应用/物种/样本/稀释参考文献
  • 免疫组化-冰冻切片; 小鼠; 图 1e
  • 免疫印迹; 小鼠; 图 s3c
Fukata Y, Chen X, Chiken S, Hirano Y, Yamagata A, Inahashi H, et al. LGI1-ADAM22-MAGUK configures transsynaptic nanoalignment for synaptic transmission and epilepsy prevention. Proc Natl Acad Sci U S A. 2021;118: pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:1000; 图 s3c
Iwata S, Morikawa M, Takei Y, Hirokawa N. An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility. Sci Adv. 2020;6: pubmed 出版商
  • 免疫组化; 小鼠; 1:500; 图 s18a
Suzuki K, Elegheert J, Song I, Sasakura H, Senkov O, Matsuda K, et al. A synthetic synaptic organizer protein restores glutamatergic neuronal circuits. Science. 2020;369: pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 4c
Lackie R, Marques Lopes J, Ostapchenko V, Good S, Choy W, van Oosten Hawle P, et al. Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-β deposition in a mouse model of Alzheimer's disease. Acta Neuropathol Commun. 2020;8:143 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 1:500; 图 3c
Yu K, Lin C, Hatcher A, Lozzi B, Kong K, Huang Hobbs E, et al. PIK3CA variants selectively initiate brain hyperactivity during gliomagenesis. Nature. 2020;578:166-171 pubmed 出版商
  • 免疫细胞化学; 人类; 图 s1d
Patzke C, Brockmann M, Dai J, Gan K, Grauel M, Fenske P, et al. Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses. Cell. 2019;179:498-513.e22 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 1:500; 图 2a
Andoh M, Shibata K, Okamoto K, Onodera J, Morishita K, Miura Y, et al. Exercise Reverses Behavioral and Synaptic Abnormalities after Maternal Inflammation. Cell Rep. 2019;27:2817-2825.e5 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1a
Schaffer T, Smith J, Cook E, Phan T, Margolis S. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep. 2018;25:2470-2483.e8 pubmed 出版商
  • 免疫印迹; 大鼠; 图 1a
Ahmad F, Salahuddin M, Alsamman K, Herzallah H, Al Otaibi S. Neonatal maternal deprivation impairs localized de novo activity-induced protein translation at the synapse in the rat hippocampus. Biosci Rep. 2018;38: pubmed 出版商
  • 免疫印迹; 大鼠; 图 1b
  • 免疫沉淀; 小鼠; 图 3a
  • 免疫细胞化学; 小鼠; 图 s1a
  • 免疫印迹; 小鼠; 图 1a
Huang H, Lin X, Liang Z, Zhao T, Du S, Loy M, et al. Cdk5-dependent phosphorylation of liprin?1 mediates neuronal activity-dependent synapse development. Proc Natl Acad Sci U S A. 2017;114:E6992-E7001 pubmed 出版商
  • 免疫细胞化学; 大鼠; 1:200; 图 6f
Fowke T, Karunasinghe R, Bai J, Jordan S, Gunn A, Dean J. Hyaluronan synthesis by developing cortical neurons in vitro. Sci Rep. 2017;7:44135 pubmed 出版商
  • 免疫组化; 大鼠; 1:200; 图 1d
Joe I, Kim J, Kim H, Hong J, Kim M, Park M. Cyclin Y-mediated transcript profiling reveals several important functional pathways regulated by Cyclin Y in hippocampal neurons. PLoS ONE. 2017;12:e0172547 pubmed 出版商
  • 免疫印迹; 人类; 图 s3
  • 免疫沉淀; 小鼠; 图 s3
Xu J, Marshall J, Fernandes H, Nomura T, Copits B, Procissi D, et al. Complete Disruption of the Kainate Receptor Gene Family Results in Corticostriatal Dysfunction in Mice. Cell Rep. 2017;18:1848-1857 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 1:200; 图 4a
  • 免疫印迹; 小鼠; 1:1000; 图 4b
Song L, Yu A, Murray K, Cortopassi G. Bipolar cell reduction precedes retinal ganglion neuron loss in a complex 1 knockout mouse model. Brain Res. 2017;1657:232-244 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 s6c
Rue L, Bañez Coronel M, Creus Muncunill J, Giralt A, Alcalá Vida R, Mentxaka G, et al. Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels. J Clin Invest. 2016;126:4319-4330 pubmed 出版商
  • 免疫印迹; 小鼠; 1:10,000; 图 11
Walkup W, Mastro T, Schenker L, Vielmetter J, Hu R, Iancu A, et al. A model for regulation by SynGAP-?1 of binding of synaptic proteins to PDZ-domain 'Slots' in the postsynaptic density. elife. 2016;5: pubmed 出版商
  • 免疫印迹; 小鼠; 1:4000; 图 3a
Chen Y, Kuo H, Bornschein U, Takahashi H, Chen S, Lu K, et al. Foxp2 controls synaptic wiring of corticostriatal circuits and vocal communication by opposing Mef2c. Nat Neurosci. 2016;19:1513-1522 pubmed 出版商
  • 免疫印迹; 小鼠; 图 7a
Fukada M, Nakayama A, Mamiya T, Yao T, Kawaguchi Y. Dopaminergic abnormalities in Hdac6-deficient mice. Neuropharmacology. 2016;110:470-479 pubmed 出版商
  • 免疫印迹; 大鼠; 1:500; 图 1
Heintz T, Eva R, Fawcett J. Regional Regulation of Purkinje Cell Dendritic Spines by Integrins and Eph/Ephrins. PLoS ONE. 2016;11:e0158558 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:200; 图 4a
Kumar A, Paeger L, Kosmas K, Kloppenburg P, Noegel A, Peche V. Neuronal Actin Dynamics, Spine Density and Neuronal Dendritic Complexity Are Regulated by CAP2. Front Cell Neurosci. 2016;10:180 pubmed 出版商
  • 免疫印迹; 小鼠; 1:2000; 图 3
Choi M, Ahn S, Yang E, Kim H, Chong Y, Kim H. Hippocampus-based contextual memory alters the morphological characteristics of astrocytes in the dentate gyrus. Mol Brain. 2016;9:72 pubmed 出版商
  • 免疫细胞化学; 大鼠; 图 3
  • 免疫印迹; 大鼠; 图 3
Yokoi N, Fukata Y, Sekiya A, Murakami T, Kobayashi K, Fukata M. Identification of PSD-95 Depalmitoylating Enzymes. J Neurosci. 2016;36:6431-44 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:500; 图 s2
Fujiwara K, Fujita Y, Kasai A, Onaka Y, Hashimoto H, Okada H, et al. Deletion of JMJD2B in neurons leads to defective spine maturation, hyperactive behavior and memory deficits in mouse. Transl Psychiatry. 2016;6:e766 pubmed 出版商
  • 免疫组化; 小鼠; 图 1f
Ryu J, Hong B, Kim Y, Yang E, Choi M, Kim H, et al. Neuregulin-1 attenuates cognitive function impairments in a transgenic mouse model of Alzheimer's disease. Cell Death Dis. 2016;7:e2117 pubmed 出版商
  • 免疫印迹; 人类; 图 4
  • 免疫印迹; 小鼠; 图 4
Ogawa F, Murphy L, Malavasi E, O Sullivan S, Torrance H, Porteous D, et al. NDE1 and GSK3? Associate with TRAK1 and Regulate Axonal Mitochondrial Motility: Identification of Cyclic AMP as a Novel Modulator of Axonal Mitochondrial Trafficking. ACS Chem Neurosci. 2016;7:553-64 pubmed 出版商
  • 免疫沉淀; 人类; 图 5
  • 免疫印迹; 人类; 图 5
Wee K, Tan F, Cheong Y, Khanna S, Low C. Ontogenic Profile and Synaptic Distribution of GluN3 Proteins in the Rat Brain and Hippocampal Neurons. Neurochem Res. 2016;41:290-7 pubmed 出版商
  • 免疫组化; 小鼠; 1:500; 图 8
Pak J, Lee E, CRAFT C. The retinal phenotype of Grk1-/- is compromised by a Crb1 rd8 mutation. Mol Vis. 2015;21:1281-94 pubmed
  • 免疫印迹; 小鼠; 1:1000
Zhang P, Fu W, Fu A, Ip N. S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength. Nat Commun. 2015;6:8665 pubmed 出版商
  • 免疫印迹; 大鼠; 1:2000; 图 5
Briz V, Liu Y, Zhu G, Bi X, Baudry M. A novel form of synaptic plasticity in field CA3 of hippocampus requires GPER1 activation and BDNF release. J Cell Biol. 2015;210:1225-37 pubmed 出版商
  • 免疫印迹; 大鼠; 图 1
Cho E, Kim D, Hur Y, Whitcomb D, Regan P, Hong J, et al. Cyclin Y inhibits plasticity-induced AMPA receptor exocytosis and LTP. Sci Rep. 2015;5:12624 pubmed 出版商
  • 免疫印迹; 大鼠; 1:1000
Farley M, Swulius M, Waxham M. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. Neuroscience. 2015;304:286-301 pubmed 出版商
  • 免疫细胞化学; 大鼠; 1:200; 图 7
Gainey M, Tatavarty V, Nahmani M, Lin H, Turrigiano G. Activity-dependent synaptic GRIP1 accumulation drives synaptic scaling up in response to action potential blockade. Proc Natl Acad Sci U S A. 2015;112:E3590-9 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:500
  • 免疫印迹; 小鼠; 1:500
Ferreira J, Schmidt J, Rio P, Águas R, Rooyakkers A, Li K, et al. GluN2B-Containing NMDA Receptors Regulate AMPA Receptor Traffic through Anchoring of the Synaptic Proteasome. J Neurosci. 2015;35:8462-79 pubmed 出版商
  • 免疫组化; 大鼠
Calafate S, Buist A, Miskiewicz K, Vijayan V, Daneels G, De Strooper B, et al. Synaptic Contacts Enhance Cell-to-Cell Tau Pathology Propagation. Cell Rep. 2015;11:1176-83 pubmed 出版商
  • 免疫组化; 小鼠
Bacaj T, Ahmad M, Jurado S, Malenka R, Südhof T. Synaptic Function of Rab11Fip5: Selective Requirement for Hippocampal Long-Term Depression. J Neurosci. 2015;35:7460-74 pubmed 出版商
  • 免疫印迹; 人类; 图 9
Russwurm C, Koesling D, Russwurm M. Phosphodiesterase 10A Is Tethered to a Synaptic Signaling Complex in Striatum. J Biol Chem. 2015;290:11936-47 pubmed 出版商
  • 免疫印迹; 大鼠; 1:2500; 图 2
Jadhav S, Katina S, Kovac A, Kazmerova Z, Novak M, Zilka N. Truncated tau deregulates synaptic markers in rat model for human tauopathy. Front Cell Neurosci. 2015;9:24 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000
Walkup W, Washburn L, Sweredoski M, Carlisle H, Graham R, Hess S, et al. Phosphorylation of synaptic GTPase-activating protein (synGAP) by Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cyclin-dependent kinase 5 (CDK5) alters the ratio of its GAP activity toward Ras and Rap GTPases. J Biol Chem. 2015;290:4908-27 pubmed 出版商
  • 免疫印迹; 小鼠; 图 5
Fitzgerald P, Pinard C, Camp M, Feyder M, Sah A, Bergstrom H, et al. Durable fear memories require PSD-95. Mol Psychiatry. 2015;20:901-12 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500; 图 3b
Yokoi N, Fukata Y, Kase D, Miyazaki T, Jaegle M, Ohkawa T, et al. Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy. Nat Med. 2015;21:19-26 pubmed 出版商
  • 免疫细胞化学; 大鼠; 2 ug/ml; 图 s5
Chazeau A, Mehidi A, Nair D, Gautier J, Leduc C, Chamma I, et al. Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion. EMBO J. 2014;33:2745-64 pubmed 出版商
  • 免疫印迹; 小鼠; 1:5000
Ho T, Vessey K, Fletcher E. Immunolocalization of the P2X4 receptor on neurons and glia in the mammalian retina. Neuroscience. 2014;277:55-71 pubmed 出版商
  • 免疫细胞化学; 人类
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, et al. Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis. J Neurosci. 2014;34:8151-63 pubmed 出版商
  • 免疫印迹; 小鼠; 图 6
Lee S, Sharma M, S dhof T, Shen J. Synaptic function of nicastrin in hippocampal neurons. Proc Natl Acad Sci U S A. 2014;111:8973-8 pubmed 出版商
  • 免疫组化; 小鼠; 1:500
  • 免疫印迹; 小鼠; 1:2000
Nikitczuk J, Patil S, Matikainen Ankney B, Scarpa J, Shapiro M, Benson D, et al. N-cadherin regulates molecular organization of excitatory and inhibitory synaptic circuits in adult hippocampus in vivo. Hippocampus. 2014;24:943-962 pubmed 出版商
  • 免疫细胞化学; 大鼠; 1:4000
Andres A, Gong X, Di K, Bota D. Low-doses of cisplatin injure hippocampal synapses: a mechanism for 'chemo' brain?. Exp Neurol. 2014;255:137-44 pubmed 出版商
  • 免疫印迹; 小鼠
Mellstrom B, Sahún I, Ruiz Nuño A, Murtra P, Gomez Villafuertes R, Savignac M, et al. DREAM controls the on/off switch of specific activity-dependent transcription pathways. Mol Cell Biol. 2014;34:877-87 pubmed 出版商
  • 免疫细胞化学; 小鼠
Dai X, Iwasaki H, Watanabe M, Okabe S. Dlx1 transcription factor regulates dendritic growth and postsynaptic differentiation through inhibition of neuropilin-2 and PAK3 expression. Eur J Neurosci. 2014;39:531-47 pubmed 出版商
  • 免疫组化; 大鼠
Ohkawa T, Fukata Y, Yamasaki M, Miyazaki T, Yokoi N, Takashima H, et al. Autoantibodies to epilepsy-related LGI1 in limbic encephalitis neutralize LGI1-ADAM22 interaction and reduce synaptic AMPA receptors. J Neurosci. 2013;33:18161-74 pubmed 出版商
  • 免疫组化; 大鼠
  • 免疫印迹; 大鼠
Yamazaki H, Kojima N, Kato K, Hirose E, Iwasaki T, Mizui T, et al. Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. J Neurochem. 2014;128:507-22 pubmed 出版商
  • 免疫细胞化学; 大鼠
Schwechter B, Rosenmund C, Tolias K. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci U S A. 2013;110:14462-7 pubmed 出版商
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Nair D, Hosy E, Petersen J, Constals A, Giannone G, Choquet D, et al. Super-resolution imaging reveals that AMPA receptors inside synapses are dynamically organized in nanodomains regulated by PSD95. J Neurosci. 2013;33:13204-24 pubmed 出版商
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Xu D, Shen W, Guo R, Xue Y, Peng W, Sima J, et al. Top3β is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation. Nat Neurosci. 2013;16:1238-47 pubmed 出版商
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Kawahara A, Kurauchi S, Fukata Y, Martínez Hernández J, Yagihashi T, Itadani Y, et al. Neuronal major histocompatibility complex class I molecules are implicated in the generation of asymmetries in hippocampal circuitry. J Physiol. 2013;591:4777-91 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 图 1
  • 免疫细胞化学; 大鼠; 图 1
  • 免疫印迹; 大鼠; 图 3
  • 免疫印迹; 人类; 图 1
Fukata Y, Dimitrov A, Boncompain G, Vielemeyer O, Perez F, Fukata M. Local palmitoylation cycles define activity-regulated postsynaptic subdomains. J Cell Biol. 2013;202:145-61 pubmed 出版商
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Aira Z, Buesa I, Garcia del Caño G, Bilbao J, Doñate F, Zimmermann M, et al. Transient, 5-HT2B receptor-mediated facilitation in neuropathic pain: Up-regulation of PKC? and engagement of the NMDA receptor in dorsal horn neurons. Pain. 2013;154:1865-77 pubmed 出版商
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  • 免疫印迹; 大鼠
Oku S, Takahashi N, Fukata Y, Fukata M. In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes. J Biol Chem. 2013;288:19816-29 pubmed 出版商
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Roppongi R, Kojima N, Hanamura K, Yamazaki H, Shirao T. Selective reduction of drebrin and actin in dendritic spines of hippocampal neurons by activation of 5-HT(2A) receptors. Neurosci Lett. 2013;547:76-81 pubmed 出版商
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Bosman E, Estabel J, Ismail O, Podrini C, White J, Steel K. Omi, a recessive mutation on chromosome 10, is a novel allele of Ostm1. Mamm Genome. 2013;24:44-53 pubmed 出版商
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Lai K, Wong A, Cheung M, Xu P, Liang Z, Lok K, et al. TrkB phosphorylation by Cdk5 is required for activity-dependent structural plasticity and spatial memory. Nat Neurosci. 2012;15:1506-15 pubmed 出版商
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Sharma M, Burré J, Sudhof T. Proteasome inhibition alleviates SNARE-dependent neurodegeneration. Sci Transl Med. 2012;4:147ra113 pubmed 出版商
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Grünewald E, Tew K, Porteous D, Thomson P. Developmental expression of orphan G protein-coupled receptor 50 in the mouse brain. ACS Chem Neurosci. 2012;3:459-72 pubmed 出版商
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Danglot L, Freret T, Le Roux N, Narboux Nême N, Burgo A, Hyenne V, et al. Vezatin is essential for dendritic spine morphogenesis and functional synaptic maturation. J Neurosci. 2012;32:9007-22 pubmed 出版商
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Mo J, Lee D, Hong S, Han S, Yeo H, Sun W, et al. Preso regulation of dendritic outgrowth through PI(4,5)P2-dependent PDZ interaction with ?Pix. Eur J Neurosci. 2012;36:1960-70 pubmed 出版商
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Gomez Varela D, Schmidt M, Schoellerman J, Peters E, Berg D. PMCA2 via PSD-95 controls calcium signaling by ?7-containing nicotinic acetylcholine receptors on aspiny interneurons. J Neurosci. 2012;32:6894-905 pubmed 出版商
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Seebohm G, Neumann S, Theiss C, Novkovic T, Hill E, Tavare J, et al. Identification of a novel signaling pathway and its relevance for GluA1 recycling. PLoS ONE. 2012;7:e33889 pubmed 出版商
  • 免疫组化-自由浮动切片; 大鼠; 1:2,000
  • 免疫组化; 大鼠; 1:2000
Noam Y, Phan L, McClelland S, Manders E, Ehrengruber M, Wadman W, et al. Distinct regional and subcellular localization of the actin-binding protein filamin A in the mature rat brain. J Comp Neurol. 2012;520:3013-34 pubmed 出版商
  • 免疫组化; 小鼠
Irie F, Badie Mahdavi H, Yamaguchi Y. Autism-like socio-communicative deficits and stereotypies in mice lacking heparan sulfate. Proc Natl Acad Sci U S A. 2012;109:5052-6 pubmed 出版商
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Mori Y, Matsui T, Furutani Y, Yoshihara Y, Fukuda M. Small GTPase Rab17 regulates dendritic morphogenesis and postsynaptic development of hippocampal neurons. J Biol Chem. 2012;287:8963-73 pubmed 出版商
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Prasad T, Weiner J. Direct and Indirect Regulation of Spinal Cord Ia Afferent Terminal Formation by the ?-Protocadherins. Front Mol Neurosci. 2011;4:54 pubmed 出版商
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Pichardo Casas I, Goff L, Swerdel M, Athie A, Davila J, Ramos Brossier M, et al. Expression profiling of synaptic microRNAs from the adult rat brain identifies regional differences and seizure-induced dynamic modulation. Brain Res. 2012;1436:20-33 pubmed 出版商
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Cichon J, Sun C, Chen B, Jiang M, Chen X, Sun Y, et al. Cofilin aggregation blocks intracellular trafficking and induces synaptic loss in hippocampal neurons. J Biol Chem. 2012;287:3919-29 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500
Briz V, Parkash J, Sánchez Redondo S, Prevot V, Suñol C. Allopregnanolone prevents dieldrin-induced NMDA receptor internalization and neurotoxicity by preserving GABA(A) receptor function. Endocrinology. 2012;153:847-60 pubmed 出版商
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Alder J, Kramer B, Hoskin C, Thakker Varia S. Brain-derived neurotrophic factor produced by human umbilical tissue-derived cells is required for its effect on hippocampal dendritic differentiation. Dev Neurobiol. 2012;72:755-65 pubmed 出版商
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  • 免疫印迹; 小鼠
Hagihara H, Ohira K, Toyama K, Miyakawa T. Expression of the AMPA Receptor Subunits GluR1 and GluR2 is Associated with Granule Cell Maturation in the Dentate Gyrus. Front Neurosci. 2011;5:100 pubmed 出版商
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Tapia L, Milnerwood A, Guo A, Mills F, Yoshida E, Vasuta C, et al. Progranulin deficiency decreases gross neural connectivity but enhances transmission at individual synapses. J Neurosci. 2011;31:11126-32 pubmed 出版商
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Ko J, Soler Llavina G, Fuccillo M, Malenka R, Sudhof T. Neuroligins/LRRTMs prevent activity- and Ca2+/calmodulin-dependent synapse elimination in cultured neurons. J Cell Biol. 2011;194:323-34 pubmed 出版商
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图像
图像 1 :
赛默飞世尔 MA1-046 图像 1
Immunofluorescent analysis of PSD95 using Anti-PSD95 Monoclonal Antibody (7E3-1B8) (Product# MA1-046) shows staining in C6 Cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 (Product# MA1-046) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
PSD-95单克隆抗体(7E3-1B8)
目录# :
MA1-046
规格 :
100微升
价格 :
美国449.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
人类, 小鼠, 大鼠, 非洲爪蛙
应用 :
Flow Cytometry: 2 µg/test, Immunocytochemistry: 1:500, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:2,000
物种 :
人类, 小鼠, 大鼠, 非洲爪蛙
克隆 :
7E3-1B8
抗体亚型 :
IgG1, kappa
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
免疫原 :
纯化重组大鼠PSD-95
格式 :
液体
应用w/稀释 :
Flow Cytometry: 2 µg/test, Immunocytochemistry: 1:500, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:2,000
别名 :
discs large homolog 4; discs large MAGUK scaffold protein 4; discs, large homolog 4; discs, large homolog 4 (Drosophila); disks large homolog 4; Dlg4; Dlgh4; FLJ97752; FLJ98574; post synaptic density; postsynaptic density protein 95; post-synaptic density protein 95; PSD95; PSD-95; PSD-95 alpha 2b; PSD-95 beta; Sap90; SAP-90; SAP90A; Synapse-associated protein 90; synapse-associated protein SAP90; Tax interaction protein 15
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国