产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
EIF2S1单克隆抗体(5A5)
目录 :
MA1-079
规格 :
100微克
价格 :
美国399.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5A5
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 2
| 出版应用/物种/样本/稀释 | 参考文献 |
|---|---|
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图像
图像 1 :
Western blot analysis of eIF2S1 was performed by loading 80ug of the indicated cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an eIF2S1 monoclonal antibody (Product # MA1-079) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
产品信息
产品类型 :
抗体
产品名称 :
EIF2S1单克隆抗体(5A5)
目录# :
MA1-079
规格 :
100微克
价格 :
美国399.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Human, Non-human primate, Rat
应用 :
Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1:1,000
物种 :
Human, Non-human primate, Rat
克隆 :
5A5
抗体亚型 :
IgG1
储存 :
-20°C
描述 :
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
免疫原 :
Full length human EIF2S1 produced in HEK293T cells.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1:1,000
别名 :
0910001O23Rik; 2410026C18Rik; 35kDa; EIF2; EIF-2; eIF2 alpha; eIF2 alpha subunit; EIF2A; eIF-2A; eIF2alpha; EIF-2alpha; eIF-2-alpha; EIF2S1; eif2s1b; Eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2 subunit 1b; eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha; eukaryotic translation initiation factor 2, subunit 1 alpha b; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A; fb19d01; translation initiation factor subunit; wu:fb19d01
更多信息或购买 :
公司信息
赛默飞世尔