产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
CREB单克隆抗体(LB9)
目录 :
MA1-083
规格 :
100微克
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
LB9
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 4
| 出版应用/物种/样本/稀释 | 参考文献 |
|---|---|
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图像
图像 1 :
Immunofluorescent analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB (Product # MA1-083), at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
图像 2 :
Immunohistochemistry was performed on cancer biopsies of deparaffinized human bladder and rectal tissue. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed in PBS and blocked in 10% normal goat serum for 20 minutes at room temperature. Tissues were probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing CREB (Product # MA1-083) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBS. Colorimetric detection was performed using metal enhanced DAB. Images are displayed at 40X magnification. Results demonstrate strong nuclear localization of CREB.
图像 3 :
Western Blot analysis of CREB in HepG2 cells using a mouse monoclonal antibody recognizing CREB (Product #MA1-083).
产品信息
产品类型 :
抗体
产品名称 :
CREB单克隆抗体(LB9)
目录# :
MA1-083
规格 :
100微克
价格 :
美国425.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Human, Mouse, Non-human primate, Rat
应用 :
ChIP assay: 2.5 µg/1x10^6 cells, ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 5 µg/mL, Immunohistochemistry: 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:500
物种 :
Human, Mouse, Non-human primate, Rat
克隆 :
LB9
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.
免疫原 :
重组人类CREB-A蛋白
格式 :
液体
应用w/稀释 :
ChIP assay: 2.5 µg/1x10^6 cells, ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 5 µg/mL, Immunohistochemistry: 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:500
别名 :
2310001E10Rik; 3526402H21Rik; active transcription factor CREB; AV083133; cAMP response element binding protein 1; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; Creb-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein
更多信息或购买 :
公司信息
赛默飞世尔