产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
ERK2单克隆抗体(6F8)
目录 :
MA1-099
规格 :
100微克
价格 :
美国375.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
6F8
反应物种 :
人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
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图像
图像 1 :
Western blot analysis of MAPK1 was performed by loading 25µg of A431 and K562 whole cell lysate and 10µg of HEK293T overexpression lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing MAPK1 (Product #MA1-099) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #32430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075).
图像 2 :
Immunofluorescent analysis of MAPK1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing MAPK1 (Product# MA1-099), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.
产品信息
产品类型 :
抗体
产品名称 :
ERK2单克隆抗体(6F8)
目录# :
MA1-099
规格 :
100微克
价格 :
美国375.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Canine, Human, Mouse, Non-human primate, Rat
应用 :
ChIP assay: 1-3 µL, Flow Cytometry: 1-10 µg/mL, Immunocytochemistry: 1:200, Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: 2 µg/500 µL, Western Blot: 1-3 µg/mL
物种 :
Canine, Human, Mouse, Non-human primate, Rat
克隆 :
6F8
抗体亚型 :
IgG2a
储存 :
-20°C
描述 :
ERK2 (also known as MAPK1) is a member of the MAP kinase subfamily of Ser/Thr protein kinases. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
免疫原 :
Protein expressed in 293T cells transfected with human MAPK1 expression vector
格式 :
液体
应用w/稀释 :
ChIP assay: 1-3 µL, Flow Cytometry: 1-10 µg/mL, Immunocytochemistry: 1:200, Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: 2 µg/500 µL, Western Blot: 1-3 µg/mL
别名 :
9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK2; ERK-2; ERT1; extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-2; extracellular-signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; Mapk; MAPK 1; MAPK 2; Mapk1; MAPK2; mitogen activated protein kinase 1; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; P42MAPK; p42-MAPK; Prkm1; PRKM2; protein tyrosine kinase ERK2
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公司信息
赛默飞世尔