产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
鼠双微基因2单克隆抗体(IF2)
目录 :
MA1-113
规格 :
100微克
价格 :
美国620.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
IF2
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片, 核糖核酸免疫沉淀
更多信息或购买 :
文章摘录数: 24
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| Amiot A, Dokmak S, Sauvanet A, Vilgrain V, Bringuier P, Scoazec J, et al. Sporadic desmoid tumor. An exceptional cause of cystic pancreatic lesion. JOP. 2008;9:339-45 pubmed
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| Bosco M, Allia E, Coindre J, Odasso C, Pagani A, Pacchioni D. alpha-fetoprotein expression in a dedifferentiated liposarcoma. Virchows Arch. 2006;448:517-20 pubmed
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| Mentzel T, Toennissen J, Rutten A, Schaller J. Palmar atypical lipomatous tumour with spindle cell features (well-differentiated spindle cell liposarcoma): a rare neoplasm arising in an unusual anatomical location. Virchows Arch. 2005;446:300-4 pubmed
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| Coindre J, Hostein I, Maire G, Derré J, Guillou L, Leroux A, et al. Inflammatory malignant fibrous histiocytomas and dedifferentiated liposarcomas: histological review, genomic profile, and MDM2 and CDK4 status favour a single entity. J Pathol. 2004;203:822-30 pubmed
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| Coindre J, Mariani O, Chibon F, Mairal A, De Saint Aubain Somerhausen N, Favre Guillevin E, et al. Most malignant fibrous histiocytomas developed in the retroperitoneum are dedifferentiated liposarcomas: a review of 25 cases initially diagnosed as malignant fibrous histiocytoma. Mod Pathol. 2003;16:256-62 pubmed
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图像
图像 1 :
Immunofluorescent analysis of MDM2 (green) in HeLa cells either left untreated (left panel) or treated with 50nM Nocodazole (right panel) for 16 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a MDM2 monoclonal antibody (Product # MA1-113) at a concentration of 1ug/ml for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
产品信息
产品类型 :
抗体
产品名称 :
鼠双微基因2单克隆抗体(IF2)
目录# :
MA1-113
规格 :
100微克
价格 :
美国620.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
人类
应用 :
Immunocytochemistry: 1-5 µg/mL, Immunohistochemistry (Frozen): 1-5 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:500, Immunoprecipitation: 1-5 µg/mL, RNA Immunoprecipitation: Assay-dependent, Western Blot: 0.5-2 µg/mL
物种 :
人类
克隆 :
IF2
抗体亚型 :
IgG2b
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.
免疫原 :
人类鼠双微基因2
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1-5 µg/mL, Immunohistochemistry (Frozen): 1-5 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:500, Immunoprecipitation: 1-5 µg/mL, RNA Immunoprecipitation: Assay-dependent, Western Blot: 0.5-2 µg/mL
别名 :
1700007J15Rik; AA415488; ACTFS; double minute 2; double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; HGNC:6973; human homolog of; MDM2; Mdm-2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog; MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53 E3 ubiquitin protein ligase; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; transformed mouse 3T3 cell double minute 2
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公司信息
赛默飞世尔