产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
雌激素受体α单克隆抗体(TE111.5D11)
目录 :
MA1-12692
规格 :
100µg
价格 :
美国558.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
TE111.5D11
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠, , , , , ,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 中和反应, 免疫沉淀, 流式细胞仪, EMSA, 染色质免疫沉淀 , 免疫组化-石蜡切片, 免疫组化-冰冻切片, 抑制或激活实验
更多信息或购买 :
文章摘录数: 68
出版应用/物种/样品/稀释参考文献
  • 免疫组化-冰冻切片; 人类; 图 1a
  • 免疫印迹; 小鼠; 图 1d
Svoronos N, Perales Puchalt A, Allegrezza M, Rutkowski M, Payne K, Tesone A, et al. Tumor Cell-Independent Estrogen Signaling Drives Disease Progression through Mobilization of Myeloid-Derived Suppressor Cells. Cancer Discov. 2017;7:72-85 pubmed 出版商
  • 免疫印迹; 人类; 图 s5c
Aiello A, Bacci L, Re A, Ripoli C, Pierconti F, Pinto F, et al. MALAT1 and HOTAIR Long Non-Coding RNAs Play Opposite Role in Estrogen-Mediated Transcriptional Regulation in Prostate Cancer Cells. Sci Rep. 2016;6:38414 pubmed 出版商
  • 流式细胞仪; 人类; 图 1a
Nanbo A, Kachi K, Yoshiyama H, Ohba Y. Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission rather than a virological synapse. J Gen Virol. 2016;97:2989-3006 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 1a
Garcia Cuellar M, Büttner C, Bartenhagen C, Dugas M, Slany R. Leukemogenic MLL-ENL Fusions Induce Alternative Chromatin States to Drive a Functionally Dichotomous Group of Target Genes. Cell Rep. 2016;15:310-22 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 1h
  • 免疫印迹; 小鼠; 图 1d
Malaterre J, Pereira L, Putoczki T, Millen R, Paquet Fifield S, Germann M, et al. Intestinal-specific activatable Myb initiates colon tumorigenesis in mice. Oncogene. 2016;35:2475-84 pubmed 出版商
  • 免疫组化-石蜡切片; 鸡; 4 ug/ml; 图 3
González Morán M. Immunohistochemical localization of progesterone receptor isoforms and estrogen receptor alpha in the chicken oviduct magnum during development. Acta Histochem. 2015;117:681-7 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 1h
  • 免疫印迹; 小鼠; 图 1d
Slaats G, Saldivar J, Bacal J, Zeman M, Kile A, Hynes A, et al. DNA replication stress underlies renal phenotypes in CEP290-associated Joubert syndrome. J Clin Invest. 2015;125:3657-66 pubmed 出版商
  • 免疫组化; 鸡; 4 ug/ml
González Morán M. Changes in the cellular localization of estrogen receptor alpha in the growing and regressing ovaries of Gallus domesticus during development. Biochem Biophys Res Commun. 2014;447:197-204 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Gagnidze K, Weil Z, Faustino L, Schaafsma S, Pfaff D. Early histone modifications in the ventromedial hypothalamus and preoptic area following oestradiol administration. J Neuroendocrinol. 2013;25:939-55 pubmed 出版商
  • EMSA; 人类
Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Miranda T, Voss T, Sung M, Baek S, John S, HAWKINS M, et al. Reprogramming the chromatin landscape: interplay of the estrogen and glucocorticoid receptors at the genomic level. Cancer Res. 2013;73:5130-9 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Bhat Nakshatri P, Song E, Collins N, Uversky V, Dunker A, O Malley B, et al. Interplay between estrogen receptor and AKT in estradiol-induced alternative splicing. BMC Med Genomics. 2013;6:21 pubmed 出版商
  • 免疫印迹; 人类; 1:200
Arao Y, Hamilton K, Coons L, Korach K. Estrogen receptor ? L543A,L544A mutation changes antagonists to agonists, correlating with the ligand binding domain dimerization associated with DNA binding activity. J Biol Chem. 2013;288:21105-16 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Maethner E, Garcia Cuellar M, Breitinger C, Takacova S, Divoky V, Hess J, et al. MLL-ENL inhibits polycomb repressive complex 1 to achieve efficient transformation of hematopoietic cells. Cell Rep. 2013;3:1553-66 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Kharbanda A, Rajabi H, Jin C, Raina D, Kufe D. Oncogenic MUC1-C promotes tamoxifen resistance in human breast cancer. Mol Cancer Res. 2013;11:714-23 pubmed 出版商
  • 免疫组化; 鸡
Katayama M, Fukuda T, Narabara K, Abe A, Kondo Y. Localization of estrogen receptor in the central lymphoid organs of chickens during the late stage of embryogenesis. Biosci Biotechnol Biochem. 2012;76:2003-7 pubmed
  • 染色质免疫沉淀 ; 人类; 1:35
Privette Vinnedge L, Ho S, Wikenheiser Brokamp K, Wells S. The DEK oncogene is a target of steroid hormone receptor signaling in breast cancer. PLoS ONE. 2012;7:e46985 pubmed 出版商
  • 免疫组化; 小鼠; 1:100
  • 免疫印迹; 小鼠; 1:100
Kam M, Lee K, Tam P, Lui V. Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons. PLoS ONE. 2012;7:e35799 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
He H, Meyer C, Chen M, Jordan V, Brown M, Liu X. Differential DNase I hypersensitivity reveals factor-dependent chromatin dynamics. Genome Res. 2012;22:1015-25 pubmed 出版商
  • 免疫印迹; 人类
Tuttle R, Miller K, Maiorano J, Termuhlen P, Gao Y, Berberich S. Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence. Int J Cancer. 2012;130:2291-9 pubmed 出版商
  • 免疫沉淀; 小鼠
Miranda Carboni G, Guemes M, Bailey S, Anaya E, Corselli M, Peault B, et al. GATA4 regulates estrogen receptor-alpha-mediated osteoblast transcription. Mol Endocrinol. 2011;25:1126-36 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Zhao L, Glazov E, Pattabiraman D, Al Owaidi F, Zhang P, Brown M, et al. Integrated genome-wide chromatin occupancy and expression analyses identify key myeloid pro-differentiation transcription factors repressed by Myb. Nucleic Acids Res. 2011;39:4664-79 pubmed 出版商
  • EMSA; 人类
Shu F, Sidell N, Yang D, Kallen C. The tri-nucleotide spacer sequence between estrogen response element half-sites is conserved and modulates ERalpha-mediated transcriptional responses. J Steroid Biochem Mol Biol. 2010;120:172-9 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Badve S, Collins N, Bhat Nakshatri P, Turbin D, Leung S, Thorat M, et al. Subcellular localization of activated AKT in estrogen receptor- and progesterone receptor-expressing breast cancers: potential clinical implications. Am J Pathol. 2010;176:2139-49 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
  • EMSA; 人类
Mason C, Shu F, Wang C, Session R, Kallen R, Sidell N, et al. Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res. 2010;38:2355-68 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100
Salama S, Kamel M, Botting S, Salih S, Borahay M, Hamed A, et al. Catechol-o-methyltransferase expression and 2-methoxyestradiol affect microtubule dynamics and modify steroid receptor signaling in leiomyoma cells. PLoS ONE. 2009;4:e7356 pubmed 出版商
  • 免疫印迹; 人类
Flamini M, Sanchez A, Goglia L, Tosi V, Genazzani A, Simoncini T. Differential actions of estrogen and SERMs in regulation of the actin cytoskeleton of endometrial cells. Mol Hum Reprod. 2009;15:675-85 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Tallack M, Keys J, Humbert P, Perkins A. EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2. J Biol Chem. 2009;284:20966-74 pubmed 出版商
  • 免疫细胞化学; 小鼠
  • 免疫印迹; 小鼠
Milanesi L, Vasconsuelo A, de Boland A, Boland R. Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue. Steroids. 2009;74:489-97 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Lupien M, Eeckhoute J, Meyer C, Krum S, Rhodes D, Liu X, et al. Coactivator function defines the active estrogen receptor alpha cistrome. Mol Cell Biol. 2009;29:3413-23 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
  • EMSA; 人类
Wang C, Yu J, Kallen C. Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells. PLoS ONE. 2008;3:e3523 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Giretti M, Fu X, De Rosa G, Sarotto I, Baldacci C, Garibaldi S, et al. Extra-nuclear signalling of estrogen receptor to breast cancer cytoskeletal remodelling, migration and invasion. PLoS ONE. 2008;3:e2238 pubmed 出版商
  • 抑制或激活实验; 小鼠
  • 免疫细胞化学; 小鼠; 1:50
  • 免疫印迹; 小鼠; 1:400
Milanesi L, Russo de Boland A, Boland R. Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line. J Cell Biochem. 2008;104:1254-73 pubmed 出版商
  • 免疫细胞化学; 小鼠
Krum S, Miranda Carboni G, Hauschka P, Carroll J, Lane T, Freedman L, et al. Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival. EMBO J. 2008;27:535-45 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Caslini C, Yang Z, El Osta M, Milne T, Slany R, Hess J. Interaction of MLL amino terminal sequences with menin is required for transformation. Cancer Res. 2007;67:7275-83 pubmed
  • 染色质免疫沉淀 ; 人类
Tallack M, Keys J, Perkins A. Erythroid Kruppel-like factor regulates the G1 cyclin dependent kinase inhibitor p18INK4c. J Mol Biol. 2007;369:313-21 pubmed
  • 染色质免疫沉淀 ; 大鼠; 2 ug
  • 免疫印迹; 大鼠; 1:300
Kazi A, Koos R. Estrogen-induced activation of hypoxia-inducible factor-1alpha, vascular endothelial growth factor expression, and edema in the uterus are mediated by the phosphatidylinositol 3-kinase/Akt pathway. Endocrinology. 2007;148:2363-74 pubmed
  • 染色质免疫沉淀 ; 人类
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Hirano S, Furutama D, Hanafusa T. Physiologically high concentrations of 17beta-estradiol enhance NF-kappaB activity in human T cells. Am J Physiol Regul Integr Comp Physiol. 2007;292:R1465-71 pubmed
  • 免疫印迹; 小鼠; 1:2000
  • 染色质免疫沉淀 ; 人类; 5-10 ug
Zhang Z, Teng C. Interplay between estrogen-related receptor alpha (ERRalpha) and gamma (ERRgamma) on the regulation of ERRalpha gene expression. Mol Cell Endocrinol. 2007;264:128-41 pubmed
  • 染色质免疫沉淀 ; 人类
Carroll J, Meyer C, Song J, Li W, Geistlinger T, Eeckhoute J, et al. Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006;38:1289-97 pubmed
  • 免疫沉淀; 大鼠; 1:250
Mannella P, Brinton R. Estrogen receptor protein interaction with phosphatidylinositol 3-kinase leads to activation of phosphorylated Akt and extracellular signal-regulated kinase 1/2 in the same population of cortical neurons: a unified mechanism of estrogen action. J Neurosci. 2006;26:9439-47 pubmed
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Simoncini T, Scorticati C, Mannella P, Fadiel A, Giretti M, Fu X, et al. Estrogen receptor alpha interacts with Galpha13 to drive actin remodeling and endothelial cell migration via the RhoA/Rho kinase/moesin pathway. Mol Endocrinol. 2006;20:1756-71 pubmed
  • 免疫沉淀; 人类; 1:30
  • 免疫印迹; 人类
Cheng A, Jin V, Fan M, Smith L, Liyanarachchi S, Yan P, et al. Combinatorial analysis of transcription factor partners reveals recruitment of c-MYC to estrogen receptor-alpha responsive promoters. Mol Cell. 2006;21:393-404 pubmed
  • 免疫组化; 大鼠; 1:150
Shirai M, Yamanaka M, Shiina H, Igawa M, Kawakami T, Ishii N, et al. Vascular endothelial growth factor restores erectile function through modulation of the insulin-like growth factor system and sex hormone receptors in diabetic rat. Biochem Biophys Res Commun. 2006;341:755-62 pubmed
  • 染色质免疫沉淀 ; 小鼠
Milne T, Martin M, Brock H, Slany R, Hess J. Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications. Cancer Res. 2005;65:11367-74 pubmed
  • 染色质免疫沉淀 ; 人类
Jeffy B, Hockings J, Kemp M, Morgan S, Hager J, Beliakoff J, et al. An estrogen receptor-alpha/p300 complex activates the BRCA-1 promoter at an AP-1 site that binds Jun/Fos transcription factors: repressive effects of p53 on BRCA-1 transcription. Neoplasia. 2005;7:873-82 pubmed
  • 免疫沉淀; 人类
Wessler S, Otto C, Wilck N, Stangl V, Fritzemeier K. Identification of estrogen receptor ligands leading to activation of non-genomic signaling pathways while exhibiting only weak transcriptional activity. J Steroid Biochem Mol Biol. 2006;98:25-35 pubmed
  • 染色质免疫沉淀 ; 人类
Carroll J, Liu X, Brodsky A, Li W, Meyer C, Szary A, et al. Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1. Cell. 2005;122:33-43 pubmed
  • 免疫沉淀; 人类
Sentis S, Le Romancer M, Bianchin C, Rostan M, Corbo L. Sumoylation of the estrogen receptor alpha hinge region regulates its transcriptional activity. Mol Endocrinol. 2005;19:2671-84 pubmed
  • 染色质免疫沉淀 ; 人类
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Park K, Krishnan V, O Malley B, Yamamoto Y, Gaynor R. Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation. Mol Cell. 2005;18:71-82 pubmed
Stokes K, Alston Mills B, Teng C. Estrogen response element and the promoter context of the human and mouse lactoferrin genes influence estrogen receptor alpha-mediated transactivation activity in mammary gland cells. J Mol Endocrinol. 2004;33:315-34 pubmed
Capiati D, Rossi A, Picotto G, Benassati S, Boland R. Inhibition of serum-stimulated mitogen activated protein kinase by 1alpha,25(OH)2-vitamin D3 in MCF-7 breast cancer cells. J Cell Biochem. 2004;93:384-97 pubmed
Kirma N, Luthra R, Jones J, Liu Y, Nair H, Mandava U, et al. Overexpression of the colony-stimulating factor (CSF-1) and/or its receptor c-fms in mammary glands of transgenic mice results in hyperplasia and tumor formation. Cancer Res. 2004;64:4162-70 pubmed
Flötotto T, Niederacher D, Hohmann D, Heimerzheim T, Dall P, Djahansouzi S, et al. Molecular mechanism of estrogen receptor (ER)alpha-specific, estradiol-dependent expression of the progesterone receptor (PR) B-isoform. J Steroid Biochem Mol Biol. 2004;88:131-42 pubmed
Letting D, Chen Y, Rakowski C, Reedy S, Blobel G. Context-dependent regulation of GATA-1 by friend of GATA-1. Proc Natl Acad Sci U S A. 2004;101:476-81 pubmed
Wu K, Tobias M, Kelley D. Estrogen receptor expression in laryngeal muscle in relation to estrogen-dependent increases in synapse strength. Neuroendocrinology. 2003;78:72-80 pubmed
Simoncini T, Rabkin E, Liao J. Molecular basis of cell membrane estrogen receptor interaction with phosphatidylinositol 3-kinase in endothelial cells. Arterioscler Thromb Vasc Biol. 2003;23:198-203 pubmed
DiRenzo J, Signoretti S, Nakamura N, Rivera Gonzalez R, Sellers W, Loda M, et al. Growth factor requirements and basal phenotype of an immortalized mammary epithelial cell line. Cancer Res. 2002;62:89-98 pubmed
González Agüero G, Ondarza R, Gamboa Dominguez A, Cerbon M, Camacho Arroyo I. Progesterone receptor isoforms expression pattern in human astrocytomas. Brain Res Bull. 2001;56:43-8 pubmed
Schlegel A, Wang C, Pestell R, Lisanti M. Ligand-independent activation of oestrogen receptor alpha by caveolin-1. Biochem J. 2001;359:203-10 pubmed
Monje P, Zanello S, Holick M, Boland R. Differential cellular localization of estrogen receptor alpha in uterine and mammary cells. Mol Cell Endocrinol. 2001;181:117-29 pubmed
Biswas D, Cruz A, Pettit N, Mutter G, Pardee A. A therapeutic target for hormone-independent estrogen receptor-positive breast cancers. Mol Med. 2001;7:59-67 pubmed
Kirma N, Gill K, Mandava U, Tekmal R. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice. Cancer Res. 2001;61:1910-8 pubmed
Klinge C. Role of estrogen receptor ligand and estrogen response element sequence on interaction with chicken ovalbumin upstream promoter transcription factor (COUP-TF). J Steroid Biochem Mol Biol. 1999;71:1-19 pubmed
Biswas D, Reddy P, Pickard M, Makkad B, Pettit N, Pardee A. Calmodulin is essential for estrogen receptor interaction with its motif and activation of responsive promoter. J Biol Chem. 1998;273:33817-24 pubmed
Zwijsen R, Buckle R, Hijmans E, Loomans C, Bernards R. Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998;12:3488-98 pubmed
Biswas D, Averboukh L, Sheng S, Martin K, Ewaniuk D, Jawde T, et al. Classification of breast cancer cells on the basis of a functional assay for estrogen receptor. Mol Med. 1998;4:454-67 pubmed
Neuman E, Ladha M, Lin N, Upton T, Miller S, DiRenzo J, et al. Cyclin D1 stimulation of estrogen receptor transcriptional activity independent of cdk4. Mol Cell Biol. 1997;17:5338-47 pubmed
图像
图像 1 :
赛默飞世尔 MA1-12692 图像 1
产品信息
产品类型 :
抗体
产品名称 :
雌激素受体α单克隆抗体(TE111.5D11)
目录# :
MA1-12692
规格 :
100µg
价格 :
美国558.00
克隆性 :
单克隆
纯度 :
蛋白质A/G
宿主 :
小鼠
反应物种 :
Bovine, Canine, Chicken, Hamster, Human, Mouse, Ovine, Porcine, Rabbit, Rat
应用 :
ChIP assay: Assay Dependent, Gel Shift: Assay Dependent, Immunoprecipitation: 2 µg/mL protein lysate, Neutralization: Assay Dependent, Western Blot: 1-2 µg/mL
物种 :
Bovine, Canine, Chicken, Hamster, Human, Mouse, Ovine, Porcine, Rabbit, Rat
克隆 :
TE111.5D11
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
免疫原 :
C-terminal fragment (aa 302-595) of human ER expressed in E coli.
格式 :
Liquid
应用w/稀释 :
ChIP assay: Assay Dependent, Gel Shift: Assay Dependent, Immunoprecipitation: 2 µg/mL protein lysate, Neutralization: Assay Dependent, Western Blot: 1-2 µg/mL
别名 :
ER
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国