表皮生长因子受体单克隆抗体(H11) | 赛默飞世尔 MA1-12693 产品信息

产品简要

公司名称 :

赛默飞世尔

其他品牌 :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

产品类型 :

抗体

产品名称 :

表皮生长因子受体单克隆抗体(H11)

MA1-12693

规格 :

100微克

价格 :

美国540.00

克隆性 :

单克隆

宿主 :

小鼠

共轭标签 :

未共轭

克隆名称 :

H11

反应物种 :

人类, 小鼠

应用 :

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 抑制或激活实验

更多信息或购买 :

赛默飞世尔产品网页

文章摘录数: 57

出版应用/物种/样本/稀释	参考文献
免疫组化; 人类; 1:200; 图 6a 免疫印迹; 人类; 图 1b	Engel B, Bowser J, Broaddus R, Carson D. MUC1 stimulates EGFR expression and function in endometrial cancer. Oncotarget. 2016;7:32796-809 pubmed 出版商
免疫细胞化学; 人类; 1:200	Engel B, Constantinou P, Sablatura L, Doty N, Carson D, Farach Carson M, et al. Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures. Adv Healthc Mater. 2015;4:1664-74 pubmed 出版商
免疫沉淀; 人类免疫细胞化学; 人类免疫印迹; 人类	Kyriakakis E, Maslova K, Frachet A, Ferri N, Contini A, Pfaff D, et al. Cross-talk between EGFR and T-cadherin: EGFR activation promotes T-cadherin localization to intercellular contacts. Cell Signal. 2013;25:1044-53 pubmed 出版商
免疫细胞化学; 人类	Gupta A, Mehta R, Alimirah F, Peng X, Murillo G, WIEHLE R, et al. Efficacy and mechanism of action of Proellex, an antiprogestin in aromatase overexpressing and Letrozole resistant T47D breast cancer cells. J Steroid Biochem Mol Biol. 2013;133:30-42 pubmed 出版商
抑制或激活实验; 人类	Spangler J, Manzari M, Rosalia E, Chen T, Wittrup K. Triepitopic antibody fusions inhibit cetuximab-resistant BRAF and KRAS mutant tumors via EGFR signal repression. J Mol Biol. 2012;422:532-44 pubmed 出版商
免疫印迹; 人类	Collins D, O Donovan N, McGowan P, O SULLIVAN F, Duffy M, Crown J. Trastuzumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) in HER-2-non-amplified breast cancer cell lines. Ann Oncol. 2012;23:1788-95 pubmed 出版商
免疫印迹; 人类	Cho J, Pastorino S, Zeng Q, Xu X, Johnson W, Vandenberg S, et al. Glioblastoma-derived epidermal growth factor receptor carboxyl-terminal deletion mutants are transforming and are sensitive to EGFR-directed therapies. Cancer Res. 2011;71:7587-96 pubmed 出版商
免疫印迹; 人类; 1:2000	Hanabata Y, Nakajima Y, Morita K, Kayamori K, Omura K. Coexpression of SGLT1 and EGFR is associated with tumor differentiation in oral squamous cell carcinoma. Odontology. 2012;100:156-63 pubmed 出版商
免疫沉淀; 人类	DeFazio Eli L, Strommen K, Dao Pick T, Parry G, Goodman L, Winslow J. Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action. Breast Cancer Res. 2011;13:R44 pubmed 出版商
免疫细胞化学; 人类; 2.5 ug/ml	Eifler R, Lind J, Falkenhagen D, Weber V, Fischer M, Zeillinger R. Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept. Cytometry B Clin Cytom. 2011;80:100-11 pubmed 出版商
免疫印迹; 人类; 1:500	Sethi S, Radio N, Kotlarczyk M, Chen C, Wei Y, Jockers R, et al. Determination of the minimal melatonin exposure required to induce osteoblast differentiation from human mesenchymal stem cells and these effects on downstream signaling pathways. J Pineal Res. 2010;49:222-38 pubmed 出版商
流式细胞仪; 人类; 1:800 免疫细胞化学; 人类; 1:500, 1:800 免疫印迹; 人类	Farabegoli F, Papi A, Orlandi M. (-)-Epigallocatechin-3-gallate down-regulates EGFR, MMP-2, MMP-9 and EMMPRIN and inhibits the invasion of MCF-7 tamoxifen-resistant cells. Biosci Rep. 2011;31:99-108 pubmed 出版商
抑制或激活实验; 人类; 1,000 ug/ml	Lowe A, Ozer B, Bai Y, Bertics P, Abbott N. Design of surfaces for liquid crystal-based bioanalytical assays. ACS Appl Mater Interfaces. 2010;2:722-31 pubmed 出版商
免疫印迹; 人类	Yamada S, Yanamoto S, Kawasaki G, Mizuno A, Nemoto T. Overexpression of cortactin increases invasion potential in oral squamous cell carcinoma. Pathol Oncol Res. 2010;16:523-31 pubmed 出版商
免疫组化; 小鼠; 1:500	Ozer B, Wiepz G, Bertics P. Activity and cellular localization of an oncogenic glioblastoma multiforme-associated EGF receptor mutant possessing a duplicated kinase domain. Oncogene. 2010;29:855-64 pubmed 出版商
免疫印迹; 人类	Cetin Z, Ozbilim G, Erdogan A, Luleci G, Karauzum S. Evaluation of PTEN and Mcl-1 expressions in NSCLC expressing wild-type or mutated EGFR. Med Oncol. 2010;27:853-60 pubmed 出版商
免疫印迹; 人类	Adam L, Zhong M, Choi W, Qi W, Nicoloso M, Arora A, et al. miR-200 expression regulates epithelial-to-mesenchymal transition in bladder cancer cells and reverses resistance to epidermal growth factor receptor therapy. Clin Cancer Res. 2009;15:5060-72 pubmed 出版商
免疫印迹; 人类	Schicher N, Paulitschke V, Swoboda A, Kunstfeld R, Loewe R, Pilarski P, et al. Erlotinib and bevacizumab have synergistic activity against melanoma. Clin Cancer Res. 2009;15:3495-502 pubmed 出版商
免疫印迹; 人类	Chen Y, Chang C, Hsu H, Chiou S, Lee L, Hseu T. Sennoside B inhibits PDGF receptor signaling and cell proliferation induced by PDGF-BB in human osteosarcoma cells. Life Sci. 2009;84:915-22 pubmed 出版商
流式细胞仪; 人类免疫组化; 人类免疫印迹; 人类	Al Nedawi K, Meehan B, Kerbel R, Allison A, Rak J. Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR. Proc Natl Acad Sci U S A. 2009;106:3794-9 pubmed 出版商
免疫沉淀; 人类免疫印迹; 人类	Corkery B, Crown J, Clynes M, O Donovan N. Epidermal growth factor receptor as a potential therapeutic target in triple-negative breast cancer. Ann Oncol. 2009;20:862-7 pubmed 出版商
免疫印迹; 人类	Graner M, Alzate O, Dechkovskaia A, Keene J, Sampson J, Mitchell D, et al. Proteomic and immunologic analyses of brain tumor exosomes. FASEB J. 2009;23:1541-57 pubmed 出版商
免疫沉淀; 人类	Lowe A, Ozer B, Wiepz G, Bertics P, Abbott N. Engineering of PDMS surfaces for use in microsystems for capture and isolation of complex and biomedically important proteins: epidermal growth factor receptor as a model system. Lab Chip. 2008;8:1357-64 pubmed 出版商
免疫沉淀; 人类免疫印迹; 人类	Guix M, Faber A, Wang S, Olivares M, Song Y, Qu S, et al. Acquired resistance to EGFR tyrosine kinase inhibitors in cancer cells is mediated by loss of IGF-binding proteins. J Clin Invest. 2008;118:2609-19 pubmed 出版商
免疫印迹; 人类; 1:1000	Wu Y, Li H, Lv X, Wei Q, Li X, Liu X, et al. Overexpression of JKTBP1 induces androgen-independent LNCaP cell proliferation through activation of epidermal growth factor-receptor (EGF-R). Cell Biochem Funct. 2008;26:467-77 pubmed 出版商
免疫印迹; 人类; 1:200-1000	Black P, Brown G, Inamoto T, Shrader M, Arora A, Siefker Radtke A, et al. Sensitivity to epidermal growth factor receptor inhibitor requires E-cadherin expression in urothelial carcinoma cells. Clin Cancer Res. 2008;14:1478-86 pubmed 出版商
抑制或激活实验; 人类免疫印迹; 人类	Sakaguchi M, Murata H, Sonegawa H, Sakaguchi Y, Futami J, Kitazoe M, et al. Truncation of annexin A1 is a regulatory lever for linking epidermal growth factor signaling with cytosolic phospholipase A2 in normal and malignant squamous epithelial cells. J Biol Chem. 2007;282:35679-86 pubmed
免疫沉淀; 人类免疫印迹; 人类	Li X, Wei J, Tai H. Activation of extracellular signal-regulated kinase by 12-hydroxyheptadecatrienoic acid in prostate cancer PC3 cells. Arch Biochem Biophys. 2007;467:20-30 pubmed
免疫印迹; 小鼠	Blair J, Rauh D, Kung C, Yun C, Fan Q, Rode H, et al. Structure-guided development of affinity probes for tyrosine kinases using chemical genetics. Nat Chem Biol. 2007;3:229-38 pubmed
免疫印迹; 人类; 1:200	Roberg K, Jonsson A, Grenman R, Norberg Spaak L. Radiotherapy response in oral squamous carcinoma cell lines: evaluation of apoptotic proteins as prognostic factors. Head Neck. 2007;29:325-34 pubmed
免疫印迹; 人类	Qiu Q, Domarkas J, Banerjee R, Katsoulas A, McNamee J, Jean Claude B. Type II combi-molecules: design and binary targeting properties of the novel triazolinium-containing molecules JDD36 and JDE05. Anticancer Drugs. 2007;18:171-7 pubmed
免疫细胞化学; 小鼠	Choi S, Mendrola J, Lemmon M. EGF-independent activation of cell-surface EGF receptors harboring mutations found in gefitinib-sensitive lung cancer. Oncogene. 2007;26:1567-76 pubmed
免疫印迹; 人类	Franovic A, Robert I, Smith K, Kurban G, Pause A, Gunaratnam L, et al. Multiple acquired renal carcinoma tumor capabilities abolished upon silencing of ADAM17. Cancer Res. 2006;66:8083-90 pubmed
流式细胞仪; 人类; 1:100 免疫细胞化学; 人类; 1:20	Chen Z, Mok H, Pflugfelder S, Li D, Barry M. Improved transduction of human corneal epithelial progenitor cells with cell-targeting adenoviral vectors. Exp Eye Res. 2006;83:798-806 pubmed
免疫印迹; 人类	Fan Q, Knight Z, Goldenberg D, Yu W, Mostov K, Stokoe D, et al. A dual PI3 kinase/mTOR inhibitor reveals emergent efficacy in glioma. Cancer Cell. 2006;9:341-9 pubmed
免疫印迹; 人类	Damiano V, Caputo R, Bianco R, D Armiento F, Leonardi A, De Placido S, et al. Novel toll-like receptor 9 agonist induces epidermal growth factor receptor (EGFR) inhibition and synergistic antitumor activity with EGFR inhibitors. Clin Cancer Res. 2006;12:577-83 pubmed
免疫印迹; 人类	Márquez D, Chen H, Curran E, Welshons W, Pietras R. Estrogen receptors in membrane lipid rafts and signal transduction in breast cancer. Mol Cell Endocrinol. 2006;246:91-100 pubmed
免疫印迹; 人类; 1:3000	Kassouf W, Dinney C, Brown G, McConkey D, Diehl A, Bar Eli M, et al. Uncoupling between epidermal growth factor receptor and downstream signals defines resistance to the antiproliferative effect of Gefitinib in bladder cancer cells. Cancer Res. 2005;65:10524-35 pubmed
免疫印迹; 人类; 1:50	Saydam O, Glauser D, Heid I, Turkeri G, Hilbe M, Jacobs A, et al. Herpes simplex virus 1 amplicon vector-mediated siRNA targeting epidermal growth factor receptor inhibits growth of human glioma cells in vivo. Mol Ther. 2005;12:803-12 pubmed
酶联免疫吸附测定; 人类; 表 2	Traxler P, Allegrini P, Brandt R, Brueggen J, Cozens R, Fabbro D, et al. AEE788: a dual family epidermal growth factor receptor/ErbB2 and vascular endothelial growth factor receptor tyrosine kinase inhibitor with antitumor and antiangiogenic activity. Cancer Res. 2004;64:4931-41 pubmed
酶联免疫吸附测定; 人类; 50 ng/well; 表 1	Garcia Echeverria C, Pearson M, Marti A, Meyer T, Mestan J, Zimmermann J, et al. In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase. Cancer Cell. 2004;5:231-9 pubmed
免疫印迹; 人类; 图 2	Pedersen M, Tkach V, Pedersen N, Berezin V, Poulsen H. Expression of a naturally occurring constitutively active variant of the epidermal growth factor receptor in mouse fibroblasts increases motility. Int J Cancer. 2004;108:643-53 pubmed
	Jacob F, Ming G, Song H. Generation and biobanking of patient-derived glioblastoma organoids and their application in CAR T cell testing. Nat Protoc. 2020;15:4000-4033 pubmed 出版商
	Jacob F, Salinas R, Zhang D, Nguyen P, Schnoll J, Wong S, et al. A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity. Cell. 2020;180:188-204.e22 pubmed 出版商
	Dragowska W, Warburton C, Yapp D, Minchinton A, Hu Y, Waterhouse D, et al. HER-2/neu overexpression increases the viable hypoxic cell population within solid tumors without causing changes in tumor vascularization. Mol Cancer Res. 2004;2:606-19 pubmed
	Lammering G, Hewit T, Holmes M, Valerie K, Hawkins W, Lin P, et al. Inhibition of the type III epidermal growth factor receptor variant mutant receptor by dominant-negative EGFR-CD533 enhances malignant glioma cell radiosensitivity. Clin Cancer Res. 2004;10:6732-43 pubmed
	Matheson S, McNamee J, Wang T, Alaoui Jamali M, Tari A, Jean Claude B. The combi-targeting concept: dissection of the binary mechanism of action of the combi-triazene SMA41 in vitro and antitumor activity in vivo. J Pharmacol Exp Ther. 2004;311:1163-70 pubmed
	Cochran J, Kim Y, Olsen M, Bhandari R, Wittrup K. Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods. 2004;287:147-58 pubmed
	Fan Q, Specht K, Zhang C, Goldenberg D, Shokat K, Weiss W. Combinatorial efficacy achieved through two-point blockade within a signaling pathway-a chemical genetic approach. Cancer Res. 2003;63:8930-8 pubmed
	Planque S, Taguchi H, Burr G, Bhatia G, Karle S, Zhou Y, et al. Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity. J Biol Chem. 2003;278:20436-43 pubmed
	Gilmour L, MacLeod K, McCaig A, Sewell J, Gullick W, Smyth J, et al. Neuregulin expression, function, and signaling in human ovarian cancer cells. Clin Cancer Res. 2002;8:3933-42 pubmed
	Brahimi F, Matheson S, Dudouit F, McNamee J, Tari A, Jean Claude B. Inhibition of epidermal growth factor receptor-mediated signaling by "Combi-triazene" BJ2000, a new probe for Combi-Targeting postulates. J Pharmacol Exp Ther. 2002;303:238-46 pubmed
	Damstrup L, Wandahl Pedersen M, Bastholm L, Elling F, Skovgaard Poulsen H. Epidermal growth factor receptor mutation type III transfected into a small cell lung cancer cell line is predominantly localized at the cell surface and enhances the malignant phenotype. Int J Cancer. 2002;97:7-14 pubmed
	Lammering G, Lin P, Contessa J, Hampton J, Valerie K, Schmidt Ullrich R. Adenovirus-mediated overexpression of dominant negative epidermal growth factor receptor-CD533 as a gene therapeutic approach radiosensitizes human carcinoma and malignant glioma cells. Int J Radiat Oncol Biol Phys. 2001;51:775-84 pubmed
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图像

图像 1 :

产品信息

产品类型 :

抗体

产品名称 :

表皮生长因子受体单克隆抗体(H11)

目录# :

MA1-12693

规格 :

100微克

价格 :

美国540.00

克隆性 :

单克隆

纯度 :

蛋白质G

宿主 :

小鼠

反应物种 :

人类, 小鼠

应用 :

Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: 2 µg/mg lysate, Western Blot: 0.5-1 µg/mL

物种 :

人类, 小鼠

克隆 :

H11

抗体亚型 :

IgG1, kappa

储存 :

-20°C

描述 :

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

免疫原 :

HC2 20 d2细胞

格式 :

液体

应用w/稀释 :

Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: 2 µg/mg lysate, Western Blot: 0.5-1 µg/mL

别名 :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2

更多信息或购买 :

赛默飞世尔产品网页

公司信息

赛默飞世尔

上海浦东新金桥路27号7号楼

analyze.cn@thermofisher.com

http://www.thermo.com.cn

1084193588

公司总部: 美国