产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
ERK1单克隆抗体(12D11)
目录 :
MA1-13041
规格 :
100微升
价格 :
美国470.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
12D11
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 1
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 s6
Tai D, Ragavendran A, Manavalan P, Stortchevoi A, Seabra C, Erdin S, et al. Engineering microdeletions and microduplications by targeting segmental duplications with CRISPR. Nat Neurosci. 2016;19:517-22 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA1-13041 图像 1
Western blot analysis of ERK1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing ERK1 (Product # MA1-13041) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:25,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34077).
图像 2 :
赛默飞世尔 MA1-13041 图像 2
Immunofluorescent analysis of ERK1 (green) in untreated and siRNA knockdown treated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing ERK1 (Product # MA1-13041), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 549 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
图像 3 :
赛默飞世尔 MA1-13041 图像 3
Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873)/TBS (Product #28376) for two hours at room temperature. Tissues were probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing ERK1 (Product # MA1-13041) overnight at 4°C in a humidified chamber. Tissues were washed extensively with TBS/0.025% Triton (Product #28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product #35000) for 30 minutes at room temperature. Detection was performed using a goat anti-mouse HRP secondary antibody (Product #31430) at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB (Product #34065). Images were taken on a Zeiss Axiovision microscope at 10X magnification. Results demonstrate strong cytoplasmic localization of ERK1.
产品信息
产品类型 :
抗体
产品名称 :
ERK1单克隆抗体(12D11)
目录# :
MA1-13041
规格 :
100微升
价格 :
美国470.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Human, Mouse, Non-human primate
应用 :
ChIP Assay: 1-3 µL, Immunocytochemistry: 2 µg/mL, Immunohistochemistry: 1:100, Western Blot: 1:1,000
物种 :
Human, Mouse, Non-human primate
克隆 :
12D11
抗体亚型 :
IgG
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.
免疫原 :
Full-length human recombinant protein expressed in bacteria.
格式 :
液体
应用w/稀释 :
ChIP Assay: 1-3 µL, Immunocytochemistry: 2 µg/mL, Immunohistochemistry: 1:100, Western Blot: 1:1,000
别名 :
ERK; Erk1; ERK-1; Ert2; Esrk1; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAP kinase2; MAPK 1; MAPK 3; MAPK1; MAPK2; MAPK3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 3; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; MNK1; Mtap2k; p44; p44 MAP kinase; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; pp42/MAP kinase; PRKM3
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国