产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A单克隆抗体(H1alpha67)
目录 :
MA1-16504
规格 :
100µL
价格 :
美国381.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
H1alpha67
反应物种 :
人类, 小鼠, 大鼠, , , ,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, EMSA, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 54
出版应用/物种/样品/稀释参考文献
  • 免疫组化; 人类; 图 1b
Murakami A, Wang L, Kalhorn S, Schraml P, Rathmell W, Tan A, et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma. Oncogenesis. 2017;6:e287 pubmed 出版商
  • 免疫组化; 猪; 1:1000; 表 1
Arvola O, Haapanen H, Herajärvi J, Anttila T, Puistola U, Karihtala P, et al. Remote Ischemic Preconditioning Reduces Cerebral Oxidative Stress Following Hypothermic Circulatory Arrest in a Porcine Model. Semin Thorac Cardiovasc Surg. 2016;28:92-102 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 1
Xu X, Tan X, Hulshoff M, Wilhelmi T, Zeisberg M, Zeisberg E. Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells. FEBS Lett. 2016;590:1222-33 pubmed 出版商
  • 免疫组化; 人类; 1:200
Bánfi G, Teleki I, Nyirády P, Keszthelyi A, Romics I, Fintha A, et al. Changes of protein expression in prostate cancer having lost its androgen sensitivity. Int Urol Nephrol. 2015;47:1149-54 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:50
Schreurs L, Smit J, Pavlov K, Pultrum B, Pruim J, Groen H, et al. Prognostic impact of clinicopathological features and expression of biomarkers related to (18)F-FDG uptake in esophageal cancer. Ann Surg Oncol. 2014;21:3751-7 pubmed 出版商
  • 免疫组化; 人类
Stofas A, Levidou G, Piperi C, Adamopoulos C, Dalagiorgou G, Bamias A, et al. The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma. BMC Cancer. 2014;14:149 pubmed 出版商
  • 免疫组化; 人类; 1:10000
Choueiri T, Fay A, Gagnon R, Lin Y, Bahamón B, Brown V, et al. The role of aberrant VHL/HIF pathway elements in predicting clinical outcome to pazopanib therapy in patients with metastatic clear-cell renal cell carcinoma. Clin Cancer Res. 2013;19:5218-26 pubmed 出版商
  • 免疫组化; 人类; 1:100
van den Berg L, Crane L, van Oosten M, van Dam G, Simons A, Hofker H, et al. Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging. Int J Gynaecol Obstet. 2013;121:35-40 pubmed 出版商
  • 免疫组化; 人类; 1:50
Park J, Jin Y, Lee Y, Lee J, Lee Y, Ko Y, et al. KAI1 suppresses HIF-1? and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer. BMC Cancer. 2012;12:81 pubmed 出版商
  • 免疫组化; 大鼠; 1:200
Hsieh Y, Chou L, Chang P, Yang C, Kao M, Hong C. Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1? (HIF-1?). J Comp Neurol. 2012;520:2903-16 pubmed 出版商
  • 免疫组化; 人类
Facciabene A, Peng X, Hagemann I, Balint K, Barchetti A, Wang L, et al. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature. 2011;475:226-30 pubmed 出版商
  • 免疫组化; 小鼠
Ahn J, Joo H, Suh J, Kim H, So H, Chung J. Effects of cigarette smoking on hearing recovery from noise-induced temporary hearing threshold shifts in mice. Otol Neurotol. 2011;32:926-32 pubmed 出版商
  • 免疫组化; 人类
Takagi Y, Kikuta K, Moriwaki T, Aoki T, Nozaki K, Hashimoto N, et al. Expression of thioredoxin-1 and hypoxia inducible factor-1? in cerebral arteriovenous malformations: Possible role of redox regulatory factor in neoangiogenic property. Surg Neurol Int. 2011;2:61 pubmed 出版商
  • 免疫组化; 人类; 1:20
Kafousi M, Vrekoussis T, Tsentelierou E, Pavlakis K, Navrozoglou I, Dousias V, et al. Immunohistochemical study of the angiogenetic network of VEGF, HIF1?, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer. Pathol Oncol Res. 2012;18:33-41 pubmed 出版商
  • 免疫组化; 人类; 1:20
Zhang L, Liu Q, Lu L, Zhao X, Gao X, Wang Y. Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1? accumulation via phosphatidylinositol 3-kinase/Akt pathway. J Pharmacol Exp Ther. 2011;338:485-91 pubmed 出版商
  • 免疫组化; 小鼠
Qing G, Skuli N, Mayes P, Pawel B, Martinez D, Maris J, et al. Combinatorial regulation of neuroblastoma tumor progression by N-Myc and hypoxia inducible factor HIF-1alpha. Cancer Res. 2010;70:10351-61 pubmed 出版商
  • 免疫组化; 人类; 1:50
Takala H, Saarnio J, Wiik H, Ohtonen P, Soini Y. HIF-1? and VEGF are associated with disease progression in esophageal carcinoma. J Surg Res. 2011;167:41-8 pubmed 出版商
  • 免疫印迹; 人类
Sumiyoshi M, Kimura Y. Enhancing effects of a chromone glycoside, eucryphin, isolated from Astilbe rhizomes on burn wound repair and its mechanism. Phytomedicine. 2010;17:820-9 pubmed 出版商
  • 免疫印迹; 大鼠
Ali M, Janic B, Babajani Feremi A, Varma N, Iskander A, Anagli J, et al. Changes in vascular permeability and expression of different angiogenic factors following anti-angiogenic treatment in rat glioma. PLoS ONE. 2010;5:e8727 pubmed 出版商
  • 免疫组化; 人类; 1:100
Puputti M, Tynninen O, Pernilä P, Salmi M, Jalkanen S, Paetau A, et al. Expression of KIT receptor tyrosine kinase in endothelial cells of juvenile brain tumors. Brain Pathol. 2010;20:763-70 pubmed 出版商
  • 免疫组化; 人类
Li M, Gu Y, Zhang Z, Zhang S, Zhang D, Saleem A, et al. Vasculogenic mimicry: a new prognostic sign of gastric adenocarcinoma. Pathol Oncol Res. 2010;16:259-66 pubmed 出版商
  • 免疫印迹; 小鼠
Matsumoto K, Obara N, Ema M, Horie M, Naka A, Takahashi S, et al. Antitumor effects of 2-oxoglutarate through inhibition of angiogenesis in a murine tumor model. Cancer Sci. 2009;100:1639-47 pubmed 出版商
  • 免疫组化; 人类
Deniz H, Karakok M, Yagci F, Guldur M. Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas. Int Urol Nephrol. 2010;42:103-7 pubmed 出版商
  • 免疫组化; 小鼠; 1:50
Bailey S, Mantena S, Millender Swain T, Cakir Y, Jhala N, Chhieng D, et al. Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease. Free Radic Biol Med. 2009;46:928-38 pubmed
  • 免疫印迹; 人类
Kang M, Jung S, Kim M, Bae J, Kim H, Kim J, et al. DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1alpha in hypoxic preconditioned HepG2 cells. FEBS J. 2008;275:5969-81 pubmed 出版商
  • 免疫组化; 人类
Arbab A, Janic B, Knight R, Anderson S, Pawelczyk E, Rad A, et al. Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings. FASEB J. 2008;22:3234-46 pubmed 出版商
  • 免疫印迹; 小鼠; 1:200
  • 免疫印迹; 人类; 1:200
Rodríguez Jiménez F, Moreno Manzano V, Lucas Dominguez R, Sánchez Puelles J. Hypoxia causes downregulation of mismatch repair system and genomic instability in stem cells. Stem Cells. 2008;26:2052-62 pubmed 出版商
  • 免疫组化; 人类; 1:50
Vakkala M, Laurila J, Saarnio J, Koivukangas V, Syrjala H, Karttunen T, et al. Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis. Crit Care. 2007;11:R116 pubmed
  • 免疫细胞化学; 大鼠; 1:200
Agrawal A, Guttapalli A, Narayan S, Albert T, Shapiro I, Risbud M. Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk. Am J Physiol Cell Physiol. 2007;293:C621-31 pubmed
  • 免疫组化; 人类; 1:100
Sihto H, Tynninen O, Butzow R, Saarialho Kere U, Joensuu H. Endothelial cell KIT expression in human tumours. J Pathol. 2007;211:481-8 pubmed
  • 免疫组化; 人类
Zagzag D, Lukyanov Y, Lan L, Ali M, Esencay M, Mendez O, et al. Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion. Lab Invest. 2006;86:1221-32 pubmed
  • 免疫印迹; 人类; 1:1000
Pore N, Jiang Z, Shu H, Bernhard E, Kao G, Maity A. Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway. Mol Cancer Res. 2006;4:471-9 pubmed
  • 免疫组化; 兔; 1:4000
Mori S, Akagi M, Kikuyama A, Yasuda Y, Hamanishi C. Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system. J Orthop Res. 2006;24:653-63 pubmed
  • EMSA; 人类
Kajimura S, Aida K, Duan C. Understanding hypoxia-induced gene expression in early development: in vitro and in vivo analysis of hypoxia-inducible factor 1-regulated zebra fish insulin-like growth factor binding protein 1 gene expression. Mol Cell Biol. 2006;26:1142-55 pubmed
  • 免疫细胞化学; 人类; 1:200
Risbud M, Guttapalli A, Stokes D, Hawkins D, Danielson K, Schaer T, et al. Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem. 2006;98:152-9 pubmed
  • 免疫印迹; 大鼠
Maloyan A, Eli Berchoer L, Semenza G, Gerstenblith G, Stern M, Horowitz M. HIF-1alpha-targeted pathways are activated by heat acclimation and contribute to acclimation-ischemic cross-tolerance in the heart. Physiol Genomics. 2005;23:79-88 pubmed
  • 免疫组化; 人类
Huang G, Yang L, Lu W. Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival. World J Gastroenterol. 2005;11:1705-8 pubmed
Wang J, Yang S, Ye F, Xia X, Shao X, Xia S, et al. Hypoxia-induced Rab11-family interacting protein 4 expression promotes migration and invasion of colon cancer and correlates with poor prognosis. Mol Med Rep. 2018;17:3797-3806 pubmed 出版商
Hedborg F, Fischer Colbrie R, Ostlin N, Sandstedt B, Tran M, Maxwell P. Differentiation in neuroblastoma: diffusion-limited hypoxia induces neuro-endocrine secretory protein 55 and other markers of a chromaffin phenotype. PLoS ONE. 2010;5: pubmed 出版商
Wang Y, Pakunlu R, Tsao W, Pozharov V, Minko T. Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis. Mol Pharm. 2004;1:156-65 pubmed
Chung J, Kang H, Shin J, Kim J. Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation. Neuroreport. 2004;15:2353-6 pubmed
Acs G, Xu X, Chu C, Acs P, Verma A. Prognostic significance of erythropoietin expression in human endometrial carcinoma. Cancer. 2004;100:2376-86 pubmed
Tuomisto T, Rissanen T, Vajanto I, Korkeela A, Rutanen J, Yla Herttuala S. HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array. Atherosclerosis. 2004;174:111-20 pubmed
Ma W, Tessarollo L, Hong S, Baba M, Southon E, Back T, et al. Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene. Cancer Res. 2003;63:5320-8 pubmed
Kurokawa T, Miyamoto M, Kato K, Cho Y, Kawarada Y, Hida Y, et al. Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage. Br J Cancer. 2003;89:1042-7 pubmed
Mehrabi M, Serbecic N, Tamaddon F, Pacher R, Horvath R, Mall G, et al. Clinical benefit of prostaglandin E1-treatment of patients with ischemic heart disease: stimulation of therapeutic angiogenesis in vital and infarcted myocardium. Biomed Pharmacother. 2003;57:173-8 pubmed
Morita M, Ohneda O, Yamashita T, Takahashi S, Suzuki N, Nakajima O, et al. HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin. EMBO J. 2003;22:1134-46 pubmed
Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M. Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells. Environ Health Perspect. 2002;110 Suppl 5:783-8 pubmed
Mehrabi M, Serbecic N, Tamaddon F, Kaun C, Huber K, Pacher R, et al. Clinical and experimental evidence of prostaglandin E1-induced angiogenesis in the myocardium of patients with ischemic heart disease. Cardiovasc Res. 2002;56:214-24 pubmed
Miyazaki K, Kawamoto T, Tanimoto K, Nishiyama M, Honda H, Kato Y. Identification of functional hypoxia response elements in the promoter region of the DEC1 and DEC2 genes. J Biol Chem. 2002;277:47014-21 pubmed
Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M. Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis. Cell Biol Toxicol. 2002;18:87-96 pubmed
Kim M, Kwon H, Lee Y, Baek J, Jang J, Lee S, et al. Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes. Nat Med. 2001;7:437-43 pubmed
Yamashita K, Discher D, Hu J, Bishopric N, Webster K. Molecular regulation of the endothelin-1 gene by hypoxia. Contributions of hypoxia-inducible factor-1, activator protein-1, GATA-2, AND p300/CBP. J Biol Chem. 2001;276:12645-53 pubmed
Salnikow K, Costa M, Figg W, Blagosklonny M. Hyperinducibility of hypoxia-responsive genes without p53/p21-dependent checkpoint in aggressive prostate cancer. Cancer Res. 2000;60:5630-4 pubmed
图像
图像 1 :
赛默飞世尔 MA1-16504 图像 1
Western Blot analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts.
图像 2 :
赛默飞世尔 MA1-16504 图像 2
Immunohistochemical staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
产品信息
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A单克隆抗体(H1alpha67)
目录# :
MA1-16504
规格 :
100µL
价格 :
美国381.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
Bovine, Ferret, Human, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat, Xenopus laevis, Yeast
应用 :
ChIP assay: 1:10-1:500, ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Gel Shift: 1:1-1:100, Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry (Frozen): 1:20-1:50, Immunohistochemistry (Paraffin): 1:20-1:50, Immunoprecipitation: 1:10-1:500, Western Blot: 1:500
物种 :
Bovine, Ferret, Human, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat, Xenopus laevis, Yeast
克隆 :
H1alpha67
抗体亚型 :
IgG2b
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
免疫原 :
融合蛋白含有氨基酸残基432-528人的HIF-1alpha
格式 :
Liquid
应用w/稀释 :
ChIP assay: 1:10-1:500, ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Gel Shift: 1:1-1:100, Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry (Frozen): 1:20-1:50, Immunohistochemistry (Paraffin): 1:20-1:50, Immunoprecipitation: 1:10-1:500, Western Blot: 1:500
别名 :
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国