产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸甘油醛脱氢酶单克隆抗体(1D4)
目录 :
MA1-16757
规格 :
100µL
价格 :
399.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
1D4
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠, , pigs , 斑马鱼, 家羊
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 13
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 大鼠; 1:40,000
Yu X, Curlik D, Oh M, Yin J, Disterhoft J. CREB overexpression in dorsal CA1 ameliorates long-term memory deficits in aged rats. elife. 2017;6: pubmed 出版商
  • 免疫印迹; 小鼠; 图 4
Yang T, Wang J, Pang Y, Dang X, Ren H, Liu Y, et al. Emodin suppresses silica-induced lung fibrosis by promoting Sirt1 signaling via direct contact. Mol Med Rep. 2016;14:4643-4649 pubmed 出版商
  • 免疫印迹; 人类
Bulk E, Ay A, Hammadi M, Ouadid Ahidouch H, Schelhaas S, Hascher A, et al. Epigenetic dysregulation of KCa 3.1 channels induces poor prognosis in lung cancer. Int J Cancer. 2015;137:1306-17 pubmed 出版商
  • 免疫印迹; 人类
Xue Y, Zhang H, Wang H, Hu J, Du M, Zhu M. Host inflammatory response inhibits Escherichia coli O157:H7 adhesion to gut epithelium through augmentation of mucin expression. Infect Immun. 2014;82:1921-30 pubmed 出版商
  • 免疫印迹; 人类; 1:5000
Jandu S, Webb A, Pak A, Sevinc B, Nyhan D, Belkin A, et al. Nitric oxide regulates tissue transglutaminase localization and function in the vasculature. Amino Acids. 2013;44:261-9 pubmed 出版商
Corona C, Pasini S, Liu J, Amar F, Greene L, Shelanski M. Activating Transcription Factor 4 (ATF4) Regulates Neuronal Activity by Controlling GABABR Trafficking. J Neurosci. 2018;38:6102-6113 pubmed 出版商
Chittoor Vinod V, Lee S, Judge S, Notterpek L. Inducible HSP70 is critical in preventing the aggregation and enhancing the processing of PMP22. ASN Neuro. 2015;7: pubmed 出版商
Chen C, Woodruff M, Chen F, Chen Y, Cilluffo M, Tranchina D, et al. Modulation of mouse rod response decay by rhodopsin kinase and recoverin. J Neurosci. 2012;32:15998-6006 pubmed 出版商
Moritz O, Tam B, Papermaster D, Nakayama T. A functional rhodopsin-green fluorescent protein fusion protein localizes correctly in transgenic Xenopus laevis retinal rods and is expressed in a time-dependent pattern. J Biol Chem. 2001;276:28242-51 pubmed
Cideciyan A, Hood D, Huang Y, Banin E, Li Z, Stone E, et al. Disease sequence from mutant rhodopsin allele to rod and cone photoreceptor degeneration in man. Proc Natl Acad Sci U S A. 1998;95:7103-8 pubmed
DeCaluwé G, Degrip W. Point mutations in bovine opsin can be classified in four groups with respect to their effect on the biosynthetic pathway of opsin. Biochem J. 1996;320 ( Pt 3):807-15 pubmed
Molday R, Mackenzie D. Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes. Biochemistry. 1983;22:653-60 pubmed
Hodges R, Heaton R, Parker J, Molday L, Molday R. Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin. J Biol Chem. 1988;263:11768-75 pubmed
图像
图像 1 :
赛默飞世尔 MA1-16757 图像 1
Human neuroblastma line Sh-SY5Y stained with anti-GAPDH (MA1-16757) [green] and an anti-NF-H [red], counterstained with a fluorescent DNA probe [blue].
图像 2 :
赛默飞世尔 MA1-16757 图像 2
Sciatic nerves of mouse wild type (+/+), heterozygous (+/o) and homozygous (o/o) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for western blots. Blots were probed with MA1-16757 mouse monoclonal antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Antibody was used at dilution of 1:1,000. Signal was revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well. Note the sharp clear band at 38kDa, the expected molecular weight for GAPDH.
产品信息
产品类型 :
抗体
产品名称 :
磷酸甘油醛脱氢酶单克隆抗体(1D4)
目录# :
MA1-16757
规格 :
100µL
价格(美元) :
399.00美元
克隆性 :
单克隆
纯度 :
亲和色谱
宿主 :
小鼠
反应物种 :
Avian, Bovine, Hamster, Human, Marsupial, Mouse, Non-human primate, Ovine, Porcine, Rat, Zebrafish
应用 :
Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry: Assay-Dependent, Western Blot: 1:2000
物种 :
Avian, Bovine, Hamster, Human, Marsupial, Mouse, Non-human primate, Ovine, Porcine, Rat, Zebrafish
克隆 :
1D4
抗体亚型 :
IgM
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
免疫原 :
Extensively purified pig GAPDH.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry: Assay-Dependent, Western Blot: 1:2000
别名 :
38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; bb02e05; cb350; cb609; CDABP0047; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; fb71f08; fk58c09; G3PD; G3PDH; Gapd; GAPD2; gapdh; GAPDH2; GAPDH-2; GAPDHS; GAPDS; Gapd-s; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase GAPDH; glyceraldehyde-3-phosphate dehydrogenase like-17 protein; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-phosphate-dehydrogenase; glycerine aldehyde 3-phosphate dehydrogenase; HEL-S-162eP; HEL-S-278; HGNC:4141; HSD35; HSD-35; I79_001391; KNC-NDS6; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; mg:bb02e05; MGC128279 protein; MGC88685; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; similar to glyceraldehyde 3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; Unknown (protein for IMAGE:8101613); wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国