产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
α微管蛋白单克隆抗体(TU-01)
目录 :
MA1-19162
规格 :
100微克
价格 :
美国325.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
TU-01
反应物种 :
African green monkey, 杜氏利什曼原虫, 人类, 小鼠, 大鼠, , pigs , 非洲爪蛙
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 34
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; pigs ; 1:500; 图 1b
Bernardini C, Bertocchi M, Zannoni A, Salaroli R, Tubon I, Dothel G, et al. Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis. BMC Vet Res. 2019;15:123 pubmed 出版商
  • 免疫印迹; 杜氏利什曼原虫; 1:5000; 图 2
Bhardwaj R, Kumar R, Singh S, Selvaraj C, Dubey V. Understanding the importance of conservative hypothetical protein LdBPK_070020 in Leishmania donovani and its role in subsistence of the parasite. Arch Biochem Biophys. 2016;596:10-21 pubmed 出版商
  • 免疫印迹; 人类; 1:400
  • 免疫印迹; 小鼠; 1:400
Sasaki M, Yoshimura Miyakoshi M, Sato Y, Nakanuma Y. A possible involvement of endoplasmic reticulum stress in biliary epithelial autophagy and senescence in primary biliary cirrhosis. J Gastroenterol. 2015;50:984-95 pubmed 出版商
  • 免疫印迹; 人类; 1:1000
Bueno Sanchez J, Peña Alzate S, Peña R, Agudelo Jaramillo B, Cadavid Jaramillo A, Chaouat G, et al. Sera from early-onset, severely preeclamptic women directly modulate HLA-E expression in the EA.hy296 endothelial cell line. J Reprod Immunol. 2014;104-105:68-79 pubmed 出版商
  • 免疫印迹; 人类; 1:1000
Kim J, Yi B, Go R, Hwang K, Nam K, Choi K. Methoxychlor and triclosan stimulates ovarian cancer growth by regulating cell cycle- and apoptosis-related genes via an estrogen receptor-dependent pathway. Environ Toxicol Pharmacol. 2014;37:1264-74 pubmed 出版商
  • 免疫印迹; 人类
Hashimoto Y, Shirane M, Matsuzaki F, Saita S, Ohnishi T, Nakayama K. Protrudin regulates endoplasmic reticulum morphology and function associated with the pathogenesis of hereditary spastic paraplegia. J Biol Chem. 2014;289:12946-61 pubmed 出版商
  • 免疫印迹; 牛; 1:500
Zannoni A, Bombardi C, Dondi F, Morini M, Forni M, Chiocchetti R, et al. Proteinase-activated receptor 2 expression in the intestinal tract of the horse. Res Vet Sci. 2014;96:464-71 pubmed 出版商
  • 免疫组化; 小鼠; 1:100
de Craene B, Denecker G, Vermassen P, Taminau J, Mauch C, Derore A, et al. Epidermal Snail expression drives skin cancer initiation and progression through enhanced cytoprotection, epidermal stem/progenitor cell expansion and enhanced metastatic potential. Cell Death Differ. 2014;21:310-20 pubmed 出版商
  • 免疫印迹; 人类; 图 5
Sasaki M, Miyakoshi M, Sato Y, Nakanuma Y. A possible involvement of p62/sequestosome-1 in the process of biliary epithelial autophagy and senescence in primary biliary cirrhosis. Liver Int. 2012;32:487-99 pubmed 出版商
  • 免疫沉淀; 人类; 图 5
  • 免疫细胞化学; 人类; 图 6
  • 免疫印迹; 人类; 图 4
Tai C, Shen S, Lee W, Liao C, Deng W, Chiou H, et al. Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells. Exp Cell Res. 2010;316:2969-81 pubmed 出版商
  • 免疫印迹; 人类; 图 2
Liao C, Luo S, Tsai C, Tsao T, Chen S, Jiang M. CAS Enhances Chemotherapeutic Drug-Induced p53 Accumulation and Apoptosis: Use of CAS for High-Sensitivity Anticancer Drug Screening. Toxicol Mech Methods. 2008;18:771-6 pubmed 出版商
  • 免疫印迹; 人类; 图 2
Sasaki M, Yamaguchi J, Ikeda H, Itatsu K, Nakanuma Y. Polycomb group protein Bmi1 is overexpressed and essential in anchorage-independent colony formation, cell proliferation and repression of cellular senescence in cholangiocarcinoma: tissue and culture studies. Hum Pathol. 2009;40:1723-30 pubmed 出版商
  • 免疫细胞化学; 人类
Miyajima N, Maruyama S, Nonomura K, Hatakeyama S. TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression. Biochem Biophys Res Commun. 2009;381:383-7 pubmed 出版商
  • 免疫印迹; 人类
Tsao T, Tsai C, Tung J, Chen S, Yue C, Liao C, et al. Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon. Mol Cell Biochem. 2009;327:163-70 pubmed 出版商
  • 免疫印迹; 人类; 1 ug/ml; 图 3
Kano S, Miyajima N, Fukuda S, Hatakeyama S. Tripartite motif protein 32 facilitates cell growth and migration via degradation of Abl-interactor 2. Cancer Res. 2008;68:5572-80 pubmed 出版商
  • 免疫印迹; 人类; 图 4
Sasaki M, Ikeda H, Itatsu K, Yamaguchi J, Sawada S, Minato H, et al. The overexpression of polycomb group proteins Bmi1 and EZH2 is associated with the progression and aggressive biological behavior of hepatocellular carcinoma. Lab Invest. 2008;88:873-82 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100; 图 4
Abdulghani J, Gu L, Dagvadorj A, Lutz J, Leiby B, Bonuccelli G, et al. Stat3 promotes metastatic progression of prostate cancer. Am J Pathol. 2008;172:1717-28 pubmed 出版商
  • 免疫印迹; 人类; 图 2
Omura T, Kaneko M, Onoguchi M, Koizumi S, Itami M, Ueyama M, et al. Novel functions of ubiquitin ligase HRD1 with transmembrane and proline-rich domains. J Pharmacol Sci. 2008;106:512-9 pubmed
  • 免疫组化; 小鼠; 图 1
Sumioka A, Saito Y, Sakuma M, Araki Y, Yamamoto T, Suzuki T. The X11L/X11beta/MINT2 and X11L2/X11gamma/MINT3 scaffold proteins shuttle between the nucleus and cytoplasm. Exp Cell Res. 2008;314:1155-62 pubmed 出版商
  • 免疫印迹; 人类; 图 1
Kaneko M, Yasui S, Niinuma Y, Arai K, Omura T, Okuma Y, et al. A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes. FEBS Lett. 2007;581:5355-60 pubmed
  • 免疫印迹; 小鼠
Nakaya T, Suzuki T. Role of APP phosphorylation in FE65-dependent gene transactivation mediated by AICD. Genes Cells. 2006;11:633-45 pubmed
  • 免疫印迹; 人类; 1:1000
Trushina E, Dyer R, Badger J, Ure D, Eide L, Tran D, et al. Mutant huntingtin impairs axonal trafficking in mammalian neurons in vivo and in vitro. Mol Cell Biol. 2004;24:8195-209 pubmed
  • 免疫印迹; 小鼠
Tsunematsu R, Nakayama K, Oike Y, Nishiyama M, Ishida N, Hatakeyama S, et al. Mouse Fbw7/Sel-10/Cdc4 is required for notch degradation during vascular development. J Biol Chem. 2004;279:9417-23 pubmed
  • 免疫细胞化学; African green monkey; 图 4
Ruddy M, Wong G, Liu X, Yamamoto H, Kasayama S, Kirkwood K, et al. Functional cooperation between interleukin-17 and tumor necrosis factor-alpha is mediated by CCAAT/enhancer-binding protein family members. J Biol Chem. 2004;279:2559-67 pubmed
  • 免疫印迹; 小鼠; 图 7
Cheng S, Shao J, Charlton Kachigian N, Loewy A, Towler D. MSX2 promotes osteogenesis and suppresses adipogenic differentiation of multipotent mesenchymal progenitors. J Biol Chem. 2003;278:45969-77 pubmed
  • 免疫印迹; 人类; 图 2b
Kudo Y, Kitajjma S, Sato S, Miyauchi M, Ogawa I, Takata T. Establishment of an oral squamous cell carcinoma cell line with high invasive and p27 degradation activities from a lymph node metastasis. Oral Oncol. 2003;39:515-20 pubmed
  • 免疫沉淀; 非洲爪蛙; 图 2
Lessman C, Kim H. Soluble tubulin complexes in oocytes of the common leopard frog, Rana pipiens, contain gamma-tubulin. Mol Reprod Dev. 2001;60:128-36 pubmed
  • 免疫细胞化学; 大鼠; 图 2
Iijima K, Ando K, Takeda S, Satoh Y, Seki T, Itohara S, et al. Neuron-specific phosphorylation of Alzheimer's beta-amyloid precursor protein by cyclin-dependent kinase 5. J Neurochem. 2000;75:1085-91 pubmed
  • 免疫细胞化学; 大鼠; 图 2
Ando K, Oishi M, Takeda S, Iijima K, Isohara T, Nairn A, et al. Role of phosphorylation of Alzheimer's amyloid precursor protein during neuronal differentiation. J Neurosci. 1999;19:4421-7 pubmed
Bernardini C, Zannoni A, Bertocchi M, Tubon I, Fernandez M, Forni M. Water/ethanol extract of Cucumis sativus L. fruit attenuates lipopolysaccharide-induced inflammatory response in endothelial cells. BMC Complement Altern Med. 2018;18:194 pubmed 出版商
Chiang Y, Park I, Terzo E, Tripathi D, Mason F, Fahey C, et al. SETD2 Haploinsufficiency for Microtubule Methylation Is an Early Driver of Genomic Instability in Renal Cell Carcinoma. Cancer Res. 2018;78:3135-3146 pubmed 出版商
Mori H, Murakami M, Tsuda T, Kameda K, Utsunomiya R, Masuda K, et al. Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytes. J Dermatol Sci. 2018;90:154-165 pubmed 出版商
Velez D, Tsui B, Goshia T, Chute C, Han A, Carter H, et al. 3D collagen architecture induces a conserved migratory and transcriptional response linked to vasculogenic mimicry. Nat Commun. 2017;8:1651 pubmed 出版商
Raciti G, Spinelli R, Desiderio A, Longo M, Parrillo L, Nigro C, et al. Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity. Sci Rep. 2017;7:43526 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA1-19162 图像 1
Immunofluorescent analysis of Phalloidin (blue) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
图像 2 :
赛默飞世尔 MA1-19162 图像 2
Immunofluorescent analysis of Phalloidin (orange) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
图像 3 :
赛默飞世尔 MA1-19162 图像 3
Western blot analysis of alpha-Tubulin was performed by loading various whole cell lysates onto a SDS-PAGE gel. Proteins were transferred to a membrane and probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a concentration of 1-2ug/ml (A) or a monoclonal antibody against a second target (B) for 1 hour at room temperature followed by an HRP-conjugated anti-mouse IgG secondary antibody. Detection was performed using a chemiluminescent substrate.
产品信息
产品类型 :
抗体
产品名称 :
α微管蛋白单克隆抗体(TU-01)
目录# :
MA1-19162
规格 :
100微克
价格 :
美国325.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Human, Many, Mouse, Porcine, Rat
应用 :
Immunocytochemistry: 1:100 - 1:500, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: 1-2 µg/mL
物种 :
Human, Many, Mouse, Porcine, Rat
克隆 :
TU-01
抗体亚型 :
IgG1
储存 :
4° C, 不冻结
描述 :
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
免疫原 :
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100 - 1:500, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: 1-2 µg/mL
别名 :
&agr-tubulin 84B; 1t; a1t; ALPHA 84C; alpha tubulin; alpha tubulin 84B; alpha[[1]] tubulin; alpha[[1]]-tubulin; alpha1; alpha1 tubulin; alpha1t; alpha1tub; alpha1tub84B; alpha1-tubulin; alpha84B; alphaT; alphat-1; alphatub; alpha-tub; alphaTub1; alphatub84; alphaTUB84B; alpha-tub84B; alphaTub84B-PA; alphatubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; alpha-tubulin 4; Alpha-tubulin 6; alphaTubulin 84B; alpha-Tubulin 84B; alpha-Tubulin at 84B; alpha-tubulin I; Alpha-tubulin II; alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin TUB1; alpha-tubulin ubiquitous; alphaTubulin84B; Alpha-Tubulin84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; AT25469p; aTub84B; a-tub84B; bA408E5.3; B-ALPHA-1; bcm948; BEST:LD32507; cb944; CG1913; CG1913-PA; chr3R:2914276. 2914416; clone 1.59; clone 2.30; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3 chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3D chain; Detyrosinated tubulin alpha-3E chain; DM1alpha; Dmel CG1913; Dmel_CG1913; DTA1; fb22g06; FLJ30169; H2 ALPHA; H2-ALPHA; hum-a-tub1; hum-a-tub2; I79_019110; K-ALPHA-1; l(3)84Bd; l(3)g3; lis3; LOC100726777; LOC100731885; LOC100857858; LOC101118307; LOC106557476; M[a]4; M[a]6; ms(3)nc33; nc33; RGD1565476; similar to alpha-tubulin isoform 1; similar to tubulin alpha 1; T; Talpha1; Talpha1 alpha-tubulin; TBA1_DROME; Testis-specific alpha-tubulin; Tub; Tub 84B; TUB1; Tub1C; Tub84B; TUBA1; Tuba-1; tuba1 protein; TUBA1/3/4; Tuba1a; tuba1a.L; tuba1a-a; tuba1a-b; TUBA1B; TUBA1C; tuba1l; tuba1l2; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; Tuba4a; TUBA5; Tuba6; tuba8.S; tubA84B; tubal2; Tubalpha1; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; tubulin alpha; tubulin alpha 1a; tubulin alpha 1a L homeolog; tubulin alpha 1b; tubulin alpha 1c; tubulin alpha 3; tubulin alpha 3c; tubulin alpha 3e; tubulin alpha 4a; tubulin alpha 6; tubulin alpha 8 S homeolog; tubulin alpha1; tubulin alpha-1 chain; tubulin alpha1 promoter; Tubulin alpha-1A chain; tubulin alpha-1B chain; Tubulin alpha-1C chain; tubulin alpha-1C chain-like; tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-5 chain; tubulin alpha-5 chain-like; tubulin alpha-6 chain; tubulin alpha-8 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; Tubulin H2-alpha; tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; tubulin, alpha 1, like 2; tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 5; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin1alpha; tubulin84B; Tubulin-alpha1; Unknown (protein for MGC:133894); unnamed protein product; wu:fb22g06; wu:fb37a10; XELAEV_18019849mg; XELAEV_18020901mg; YML085C
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国