产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
乙3微管蛋白单克隆抗体(TU-20)
目录 :
MA1-19187
规格 :
100微克
价格 :
美国491
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
TU-20
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 5
| 出版应用/物种/样本/稀释 | 参考文献 |
|---|---|
| |
| |
图像
图像 1 :
Immunofluorescent analysis of beta-3 Tubulin (green) in SH-SY5Y cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1?M staurosporine (right panel) for 3 hours and probed with a beta-3 Tubulin monoclonal antibody (Product # MA1-19187), at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight at 20X magnification.
产品信息
产品类型 :
抗体
产品名称 :
乙3微管蛋白单克隆抗体(TU-20)
目录# :
MA1-19187
规格 :
100微克
价格 :
美国491
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Human, Many, Mouse, Rat
应用 :
Flow Cytometry: 1-4 µg/mL, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 10 µg/mL, Western Blot: 1-2 µg/mL, 90 min
物种 :
Human, Many, Mouse, Rat
克隆 :
TU-20
抗体亚型 :
IgG1
储存 :
4° C, 不冻结
描述 :
The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
免疫原 :
Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide
格式 :
液体
应用w/稀释 :
Flow Cytometry: 1-4 µg/mL, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 10 µg/mL, Western Blot: 1-2 µg/mL, 90 min
别名 :
3200002H15Rik; beta 3 tubulin; beta tubulin 3; beta-4; betaIII-tubulin; CDCBM; CDCBM1; CFEOM3; CFEOM3A; class III beta-tubulin; FEOM3; I79_000194; LOC100341581; M(beta)3; M(beta)6; mc1R; melanocortin receptor 1; Neuron specific beta III Tubulin; neuron-specific class III beta-tubulin; TUBB; Tubb3; TUBB4; tubulin beta; tubulin beta 3 class III; Tubulin beta-3; tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4; Unknown (protein for MGC:134115)
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公司信息
赛默飞世尔