产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
alpha Catenin Monoclonal Antibody (1G5)
目录 :
MA1-2000
规格 :
100微克
价格 :
美国500
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
1G5
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 9
| 出版应用/物种/样本/稀释 | 参考文献 |
|---|---|
| |
Katafiasz B, Nieman M, Wheelock M, Johnson K. Characterization of cadherin-24, a novel alternatively spliced type II cadherin. J Biol Chem. 2003;278:27513-9 pubmed
| |
Wahl J, Kim Y, Cullen J, Johnson K, Wheelock M. N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem. 2003;278:17269-76 pubmed
| |
Nieman M, Kim J, Johnson K, Wheelock M. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Sci. 1999;112 ( Pt 10):1621-32 pubmed
| |
Islam S, Carey T, Wolf G, Wheelock M, Johnson K. Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion. J Cell Biol. 1996;135:1643-54 pubmed
| |
Knudsen K, Soler A, Johnson K, Wheelock M. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol. 1995;130:67-77 pubmed
| |
Sacco P, McGranahan T, Wheelock M, Johnson K. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem. 1995;270:20201-6 pubmed
|
图像
图像 1 :
Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product# MA1-2000 ) shows staining in A2058 Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Product# MA1-2000 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 2 :
Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product# MA1-2000 ) shows staining in Hela Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Product# MA1-2000 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
alpha Catenin Monoclonal Antibody (1G5)
目录# :
MA1-2000
规格 :
100微克
价格 :
美国500
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
人类
应用 :
Immunocytochemistry: 10 µg/mL, Immunofluorescence: 10 µg/mL, Immunoprecipitation: Assay dependent, Western Blot: 1:100-1:1000
物种 :
人类
克隆 :
1G5
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. It also regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Alpha catenin also functions in the control of the startle modulation.
免疫原 :
90 kDa MBP/alpha catenin fusion protein purified on amylose column.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 10 µg/mL, Immunofluorescence: 10 µg/mL, Immunoprecipitation: Assay dependent, Western Blot: 1:100-1:1000
别名 :
102 kDa cadherin-associated protein; 2010010M04Rik; AA517462; AI481747; AI988031; alpha E catenin; alpha E-catenin; Alpha N-catenin; alpha(E)-catenin; alpha(N)-catenin; alpha-catenin; Alpha-catenin-related protein; alpha-E-catenin; alphaE-catenin; alpha-N-catenin; alphaN-catenin; cadherin associated protein; Cadherin-associated protein; cadherin-associated protein, related; cancer/testis antigen 114; CAP102; CAPR; CAP-R; catenin; catenin (cadherin associated protein), alpha 1; catenin (cadherin associated protein), alpha 2; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin (cadherin-associated protein), alpha 2; catenin alpha 1; catenin alpha 2; Catenin alpha1; catenin alpha-1; Catenin alpha2; catenin alpha-2; catenin, alpha 1; Catenin-alpha1; Catenin-alpha2; Catna; Catna1; Catna2; cdf; cerebellar deficient folia; chp; CT114; Ctnna1; CTNNA2; CTNR; FLJ36832; FLJ52416; HGNC:2509; MDPT2; Neural alpha-catenin; NY-REN-13 antigen; Renal carcinoma antigen NY-REN-13
更多信息或购买 :
公司信息
赛默飞世尔