产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸化毛细血管扩张性共济失调症突变蛋白(Ser1981)单克隆抗体(10H11)
目录 :
MA1-2020
规格 :
200微克
价格 :
美国480.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
10H11
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 5
| 出版应用/物种/样本/稀释 | 参考文献 |
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Bakkenist C, Drissi R, Wu J, Kastan M, Dome J. Disappearance of the telomere dysfunction-induced stress response in fully senescent cells. Cancer Res. 2004;64:3748-52 pubmed
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图像
图像 1 :
Immunofluorescent analysis of ATM phosphorylated on pSer1981 (green) in HeLa cells left untreated (left panels) or treated with 10uM Camptothecin for 4 hours (right panels). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a phospho-ATM (pSer1981) monoclonal antibody (Product # MA1-2020), at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) (top panels). A merged image with nuclei staining (blue) using Hoechst 33342 dye (Product # 62249) is shown on bottom panels. Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
图像 2 :
Western blot analysis of phospho-ATM (pSer1981) was performed using phospho-ATM (pSer1981) monoclonal antibody (Product # MA1-2020) on gamma-irradiated HeLa cells.
产品信息
产品类型 :
抗体
产品名称 :
磷酸化毛细血管扩张性共济失调症突变蛋白(Ser1981)单克隆抗体(10H11)
目录# :
MA1-2020
规格 :
200微克
价格 :
美国480.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
人类, 小鼠
应用 :
Immunocytochemistry: 1:250, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:1,000
物种 :
人类, 小鼠
克隆 :
10H11
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.
免疫原 :
合成多肽对应于氨基酸残基S(1974)L A F E E S(p)Q S T T I S S(1988)人的毛细血管扩张性共济失调症突变蛋白激酶蛋白
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:250, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:1,000
别名 :
AI256621; AT mutated; A-T mutated; A-T mutated homolog; AT mutated protein; AT1; ATA; Ataxia t; Ataxia telangiectasia gene mutated in human beings; ataxia telangiectasia mutated; Ataxia telangiectasia mutated homolog; ATC; ATD; ATDC; ATE; Atm; ATM (phospho S1981); ATM (pS1981); ATM (pSer1981); ATM phospho S1981; ATM phospho Ser1981; ATM serine/threonine kinase; C030026E19Rik; DKFZp781A0353; EC 2.7.1.37; MGC74674; phospho ATM; phospho S1981 ATM; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1
更多信息或购买 :
公司信息
赛默飞世尔