产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸化组蛋白H2A.X(Ser139)单克隆抗体(3F2)
目录 :
MA1-2022
规格 :
100微克
价格 :
美国450.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
3F2
反应物种 :
人类, 小鼠, 牛
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 其他
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 23
出版应用/物种/样本/稀释参考文献
  • 其他; 人类; 图 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed 出版商
  • 免疫印迹; 人类; 图 s1g
Gregory M, D Alessandro A, Alvarez Calderon F, Kim J, Nemkov T, Adane B, et al. ATM/G6PD-driven redox metabolism promotes FLT3 inhibitor resistance in acute myeloid leukemia. Proc Natl Acad Sci U S A. 2016;113:E6669-E6678 pubmed
  • 免疫细胞化学; 人类; 1:2000; 图 6
Marescotti D, Gonzalez Suarez I, Acali S, Johne S, Laurent A, Frentzel S, et al. High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC). J Vis Exp. 2016;: pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 5
Vijaya Chandra S, Makhija H, Peter S, Myint Wai C, Li J, Zhu J, et al. Conservative site-specific and single-copy transgenesis in human LINE-1 elements. Nucleic Acids Res. 2016;44:e55 pubmed 出版商
  • 免疫细胞化学; 人类; 1:200; 图 5
  • 免疫印迹; 人类; 1:1000; 图 5
Hassan M, El Khattouti A, Ejaeidi A, Ma T, Day W, Espinoza I, et al. Elevated Expression of Hepatoma Up-Regulated Protein Inhibits γ-Irradiation-Induced Apoptosis of Prostate Cancer Cells. J Cell Biochem. 2016;117:1308-18 pubmed 出版商
  • 免疫细胞化学; 人类; 图 s2f
Wang P, Alvarez Perez J, Felsenfeld D, Liu H, Sivendran S, Bender A, et al. A high-throughput chemical screen reveals that harmine-mediated inhibition of DYRK1A increases human pancreatic beta cell replication. Nat Med. 2015;21:383-8 pubmed 出版商
  • 免疫细胞化学; 人类
Ferron P, Hogeveen K, Fessard V, Le Hégarat L. Comparative analysis of the cytotoxic effects of okadaic acid-group toxins on human intestinal cell lines. Mar Drugs. 2014;12:4616-34 pubmed 出版商
  • 免疫印迹; 人类
Clewell R, Sun B, Adeleye Y, Carmichael P, Efremenko A, McMullen P, et al. Profiling dose-dependent activation of p53-mediated signaling pathways by chemicals with distinct mechanisms of DNA damage. Toxicol Sci. 2014;142:56-73 pubmed 出版商
  • 免疫细胞化学; 人类
Jagirdar K, Yin K, Harrison M, Lim W, Muscat G, Sturm R, et al. The NR4A2 nuclear receptor is recruited to novel nuclear foci in response to UV irradiation and participates in nucleotide excision repair. PLoS ONE. 2013;8:e78075 pubmed 出版商
  • 免疫细胞化学; 人类; 1:1500
Geiger M, Desanglois G, Hogeveen K, Fessard V, Leprêtre T, Mondeguer F, et al. Cytotoxicity, fractionation and dereplication of extracts of the dinoflagellate Vulcanodinium rugosum, a producer of pinnatoxin G. Mar Drugs. 2013;11:3350-71 pubmed 出版商
  • 酶联免疫吸附测定; 牛
Shimada Y, Goto T, Kawamoto S, Kiso T, Katayama A, Yamanaka Y, et al. Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Biomed Chromatogr. 2008;22:13-9 pubmed
Poivre M, Antoine M, Kryshen K, Atsapkina A, Shikov A, Twyffels L, et al. Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: Aristolochia baetica and Magnolia officinalis. Toxins (Basel). 2023;15: pubmed 出版商
Marty F, Bettamin L, Thouard A, Bourgade K, Allart S, Larrieu G, et al. Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity. iScience. 2022;25:103621 pubmed 出版商
Klomp J, Lee Y, Goodwin C, Papke B, Klomp J, Waters A, et al. CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell Rep. 2021;37:110060 pubmed 出版商
Porosnicu I, Butnaru C, Tiseanu I, Stancu E, Munteanu C, Bita B, et al. Y2O3 Nanoparticles and X-ray Radiation-Induced Effects in Melanoma Cells. Molecules. 2021;26: pubmed 出版商
Yu F, Wei J, Cui X, Yu C, Ni W, Bungert J, et al. Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response. Nucleic Acids Res. 2021;49:5779-5797 pubmed 出版商
Boekema B, Stoop M, Vlig M, van Liempt J, Sobota A, Ulrich M, et al. Antibacterial and safety tests of a flexible cold atmospheric plasma device for the stimulation of wound healing. Appl Microbiol Biotechnol. 2021;105:2057-2070 pubmed 出版商
Larose H, Kent T, Ma Q, Shami A, Harerimana N, Li J, et al. Regulation of meiotic progression by Sertoli-cell androgen signaling. Mol Biol Cell. 2020;31:2841-2862 pubmed 出版商
Dijksteel G, Ulrich M, Vlig M, Sobota A, Middelkoop E, Boekema B. Safety and bactericidal efficacy of cold atmospheric plasma generated by a flexible surface Dielectric Barrier Discharge device against Pseudomonas aeruginosa in vitro and in vivo. Ann Clin Microbiol Antimicrob. 2020;19:37 pubmed 出版商
Salmina K, Bojko A, Inashkina I, Staniak K, Dudkowska M, Podlesniy P, et al. "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. Int J Mol Sci. 2020;21: pubmed 出版商
Ko T, Fujita K, Nomura S, Uemura Y, Yamada S, Tobita T, et al. Quantification of DNA Damage in Heart Tissue as a Novel Prediction Tool for Therapeutic Prognosis of Patients With Dilated Cardiomyopathy. JACC Basic Transl Sci. 2019;4:670-680 pubmed 出版商
de Moura T, Mozaffari Jovin S, Szabó C, Schmitzová J, Dybkov O, Cretu C, et al. Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell. 2018;69:979-992.e6 pubmed 出版商
Reid D, Keegan S, Leo Macias A, Watanabe G, Strande N, Chang H, et al. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proc Natl Acad Sci U S A. 2015;112:E2575-84 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA1-2022 图像 1
Immunofluorescence image of phosphorylated H2AX in gamma irradiated HeLa cells using MA1-2022.
图像 2 :
赛默飞世尔 MA1-2022 图像 2
Western blot detection of phosphorylated H2AX in gamma irradiated HeLA cell lysate using MA1-2022. Lane 1: Unphosphorylated H2AX control Lane 2: Phosphorylated H2AX
图像 3 :
赛默飞世尔 MA1-2022 图像 3
Western blot analysis of Phospho-H2AX pSer140 (MA1-2022) at a concentration of 1 ug/ml on Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) followed by HRP conjugated goat anti-mouse lgG (H+L) Secondary Antibody.
产品信息
产品类型 :
抗体
产品名称 :
磷酸化组蛋白H2A.X(Ser139)单克隆抗体(3F2)
目录# :
MA1-2022
规格 :
100微克
价格 :
美国450.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
人类, 小鼠
应用 :
ELISA: Assay-dependent, Flow Cytometry: 1 µg / 10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:400, Western Blot: 1 µg/mL
物种 :
人类, 小鼠
克隆 :
3F2
抗体亚型 :
IgG1, kappa
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
免疫原 :
Synthetic peptide sequence surrounding phosphorylated Ser140
格式 :
液体
应用w/稀释 :
ELISA: Assay-dependent, Flow Cytometry: 1 µg / 10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:400, Western Blot: 1 µg/mL
别名 :
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国