产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
6x多聚组氨酸标签单克隆抗体(HIS.H8)
目录 :
MA1-21315毫克
规格 :
1毫克
价格 :
美国1242
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
HIS.H8
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 192
参考文献
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产品信息
产品类型 :
抗体
产品名称 :
6x多聚组氨酸标签单克隆抗体(HIS.H8)
目录# :
MA1-21315毫克
规格 :
1毫克
价格 :
美国1242
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
标签
应用 :
ELISA: Assay-dependent, Immunocytochemistry: 1:500-1:2,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:3,000
物种 :
标签
克隆 :
HIS.H8
抗体亚型 :
IgG2b
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
免疫原 :
6x His synthetic peptide.
格式 :
液体
应用w/稀释 :
ELISA: Assay-dependent, Immunocytochemistry: 1:500-1:2,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:3,000
别名 :
6His tag; 6X His; His Tag; Histidine Tag
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国