产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
beta连环蛋白单克隆抗体(15B8)
目录 :
MA1-301
规格 :
100微克
价格 :
美国516.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
15B8
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
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文章摘录数: 15
| 出版应用/物种/样本/稀释 | 参考文献 |
|---|---|
| |
图像
图像 1 :
Western blot analysis of beta-Catenin was performed by loading 50µg of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a beta-Catenin monoclonal antibody (Product # MA1-301) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
图像 2 :
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Catenin beta monoclonal antibody (Product # MA1-301) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
beta连环蛋白单克隆抗体(15B8)
目录# :
MA1-301
规格 :
100微克
价格 :
美国516.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Human, Mouse, Non-human primate, Rat
应用 :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:50-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:1,000
物种 :
Human, Mouse, Non-human primate, Rat
克隆 :
15B8
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.
免疫原 :
重组beta连环蛋白蛋白
格式 :
液体
应用w/稀释 :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:50-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:1,000
别名 :
armadillo; armadillo homolog; Bcatenin; b-catenin; beta 1 88kDa; beta catenin; Betacatenin; beta-catenin; beta-catenin 1; Bfc; C20orf33; Cadherin associated protein; catenin; catenin (cadherin associated protein), beta 1; catenin (cadherin associated protein), beta 1, 88kDa; catenin (cadherin-associated protein) beta 1; catenin (cadherin-associated protein), beta 1; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin b 1; Catenin b1; catenin beta; catenin beta 1; catenin beta 1 L homeolog; Catenin beta1; Catenin beta-1; Catenin ß 1; Catenin ß1; Catnb; CHBCAT; CTNB1; CTNNB; ctnnb1; ctnnb1.L; ctnnb1-a; ctnnb1-b; dJ633O20.1; DKFZp686D02253; FLJ25606; FLJ37923; id:ibd2058; Mesc; MRD19; NAP; NYD-SP19; OK/SW-cl.35; OTTHUMP00000209289; P14L; PP8304; PRO2286; RP5-1118M15.1; wu:fb73e10; wu:fi81c06; wu:fk25h01; XELAEV_18031149mg; ß catenin; ßcatenin
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公司信息
赛默飞世尔