产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸化孕酮受体(Ser294)单克隆抗体(608)
目录 :
MA1-414
规格 :
200微克
价格 :
美国420.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
608
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 11
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 3
Hagan C, Knutson T, Lange C. A Common Docking Domain in Progesterone Receptor-B links DUSP6 and CK2 signaling to proliferative transcriptional programs in breast cancer cells. Nucleic Acids Res. 2013;41:8926-42 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:100
Bottino M, Cerliani J, Rojas P, Giulianelli S, Soldati R, Mondillo C, et al. Classical membrane progesterone receptors in murine mammary carcinomas: agonistic effects of progestins and RU-486 mediating rapid non-genomic effects. Breast Cancer Res Treat. 2011;126:621-36 pubmed 出版商
  • 免疫组化; 大鼠; 1:300
Gordon A, Aguilar R, Garrido Gracia J, Guil Luna S, Sánchez Cespedes R, Millan Y, et al. Immunoneutralization of inhibin in cycling rats increases follicle-stimulating hormone secretion, stimulates the ovary and attenuates progesterone receptor-dependent preovulatory luteinizing hormone secretion. Neuroendocrinology. 2010;91:291-301 pubmed 出版商
  • 免疫印迹; 人类
Daniel A, Lange C. Protein kinases mediate ligand-independent derepression of sumoylated progesterone receptors in breast cancer cells. Proc Natl Acad Sci U S A. 2009;106:14287-92 pubmed 出版商
  • 免疫印迹; 人类
Proietti C, Rosemblit C, Beguelin W, Rivas M, Díaz Flaqué M, Charreau E, et al. Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth. Mol Cell Biol. 2009;29:1249-65 pubmed 出版商
  • 免疫印迹; 小鼠; 1:50
Giulianelli S, Cerliani J, Lamb C, Fabris V, Bottino M, Gorostiaga M, et al. Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis. Int J Cancer. 2008;123:2518-31 pubmed 出版商
  • 免疫印迹; 人类
Daniel A, Qiu M, Faivre E, Ostrander J, Skildum A, Lange C. Linkage of progestin and epidermal growth factor signaling: phosphorylation of progesterone receptors mediates transcriptional hypersensitivity and increased ligand-independent breast cancer cell growth. Steroids. 2007;72:188-201 pubmed
  • 免疫印迹; 人类
Skildum A, Faivre E, Lange C. Progesterone receptors induce cell cycle progression via activation of mitogen-activated protein kinases. Mol Endocrinol. 2005;19:327-39 pubmed
Salatino M, Schillaci R, Proietti C, Carnevale R, Frahm I, Molinolo A, et al. Inhibition of in vivo breast cancer growth by antisense oligodeoxynucleotides to type I insulin-like growth factor receptor mRNA involves inactivation of ErbBs, PI-3K/Akt and p42/p44 MAPK signaling pathways but not modulation of progesterone receptor. Oncogene. 2004;23:5161-74 pubmed
Qiu M, Olsen A, Faivre E, Horwitz K, Lange C. Mitogen-activated protein kinase regulates nuclear association of human progesterone receptors. Mol Endocrinol. 2003;17:628-42 pubmed
Clemm D, Sherman L, Boonyaratanakornkit V, Schrader W, Weigel N, Edwards D. Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Mol Endocrinol. 2000;14:52-65 pubmed
图像
图像 1 :
赛默飞世尔 MA1-414 图像 1
Immunofluorescent analysis of Phosphorylated Progesterone Receptor using Phospho-Progesterone Receptor pSer294 Monoclonal Antibody (608) (Product# MA1-414 ) shows staining in U251 Cells. Phospho-Progesterone Receptor pSer294 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Phospho-Progesterone Receptor pSer294 (Product# MA1-414 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 2 :
赛默飞世尔 MA1-414 图像 2
Immunofluorescent analysis of Phosphorylated Progesterone Receptor using Phospho-Progesterone Receptor pSer294 Monoclonal Antibody (608) (Product# MA1-414 ) shows staining in Hela Cells. Phospho-Progesterone Receptor pSer294 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Phospho-Progesterone Receptor pSer294 (Product# MA1-414 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 3 :
赛默飞世尔 MA1-414 图像 3
Immunofluorescent analysis of Phosphorylated Progesterone Receptor using Phospho-Progesterone Receptor pSer294 Monoclonal Antibody (608) (Product# MA1-414 ) shows staining in MCF-7 Cells. Phospho-Progesterone Receptor pSer294 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Phospho-Progesterone Receptor pSer294 (Product# MA1-414 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
磷酸化孕酮受体(Ser294)单克隆抗体(608)
目录# :
MA1-414
规格 :
200微克
价格 :
美国420.00
克隆性 :
单克隆
纯度 :
纯化
宿主 :
小鼠
反应物种 :
人类
应用 :
ELISA: Assay-dependent, Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 10 µg/mL
物种 :
人类
克隆 :
608
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.
免疫原 :
Synthetic phosphopeptide corresponding to residues P(288) M A P G R S(p) P L A T T V(300) of human Progesterone Receptor.
格式 :
液体
应用w/稀释 :
ELISA: Assay-dependent, Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 10 µg/mL
别名 :
9930019P03Rik; BB114106; ENSMUSG00000074510; LOW QUALITY PROTEIN: progesterone receptor; NR3C3; Nuclear receptor subfamily 3 group C member 3; p4 receptor; PGR; pot. alt. progesterone receptor; putative; PR; PR-A; PR-B; Progesterone receptor; progesterone receptor A; progesterone-receptor; putative form B; steroid hormone receptor; testicular progesterone receptor; unnamed protein product
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国