产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A单克隆抗体(H1alpha67), 生物素
目录 :
MA1-46457
规格 :
100微升
价格 :
美国449.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
生物素
克隆名称 :
H1alpha67
反应物种 :
人类, 小鼠, 大鼠, 犬, 牛, pigs , 家羊, domestic rabbit
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
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文章摘录数: 39
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| Bailey S, Mantena S, Millender Swain T, Cakir Y, Jhala N, Chhieng D, et al. Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease. Free Radic Biol Med. 2009;46:928-38 pubmed
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| Vakkala M, Laurila J, Saarnio J, Koivukangas V, Syrjala H, Karttunen T, et al. Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis. Crit Care. 2007;11:R116 pubmed
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| Agrawal A, Guttapalli A, Narayan S, Albert T, Shapiro I, Risbud M. Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk. Am J Physiol Cell Physiol. 2007;293:C621-31 pubmed
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| Sihto H, Tynninen O, Butzow R, Saarialho Kere U, Joensuu H. Endothelial cell KIT expression in human tumours. J Pathol. 2007;211:481-8 pubmed
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| Zagzag D, Lukyanov Y, Lan L, Ali M, Esencay M, Mendez O, et al. Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion. Lab Invest. 2006;86:1221-32 pubmed
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| Pore N, Jiang Z, Shu H, Bernhard E, Kao G, Maity A. Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway. Mol Cancer Res. 2006;4:471-9 pubmed
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| Mori S, Akagi M, Kikuyama A, Yasuda Y, Hamanishi C. Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system. J Orthop Res. 2006;24:653-63 pubmed
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| Risbud M, Guttapalli A, Stokes D, Hawkins D, Danielson K, Schaer T, et al. Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem. 2006;98:152-9 pubmed
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| Huang G, Yang L, Lu W. Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival. World J Gastroenterol. 2005;11:1705-8 pubmed
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Wang Y, Pakunlu R, Tsao W, Pozharov V, Minko T. Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis. Mol Pharm. 2004;1:156-65 pubmed
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Chung J, Kang H, Shin J, Kim J. Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation. Neuroreport. 2004;15:2353-6 pubmed
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Acs G, Xu X, Chu C, Acs P, Verma A. Prognostic significance of erythropoietin expression in human endometrial carcinoma. Cancer. 2004;100:2376-86 pubmed
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Tuomisto T, Rissanen T, Vajanto I, Korkeela A, Rutanen J, Yla Herttuala S. HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array. Atherosclerosis. 2004;174:111-20 pubmed
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Ma W, Tessarollo L, Hong S, Baba M, Southon E, Back T, et al. Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene. Cancer Res. 2003;63:5320-8 pubmed
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Kurokawa T, Miyamoto M, Kato K, Cho Y, Kawarada Y, Hida Y, et al. Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage. Br J Cancer. 2003;89:1042-7 pubmed
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Morita M, Ohneda O, Yamashita T, Takahashi S, Suzuki N, Nakajima O, et al. HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin. EMBO J. 2003;22:1134-46 pubmed
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Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M. Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells. Environ Health Perspect. 2002;110 Suppl 5:783-8 pubmed
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Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M. Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis. Cell Biol Toxicol. 2002;18:87-96 pubmed
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Kim M, Kwon H, Lee Y, Baek J, Jang J, Lee S, et al. Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes. Nat Med. 2001;7:437-43 pubmed
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Yamashita K, Discher D, Hu J, Bishopric N, Webster K. Molecular regulation of the endothelin-1 gene by hypoxia. Contributions of hypoxia-inducible factor-1, activator protein-1, GATA-2, AND p300/CBP. J Biol Chem. 2001;276:12645-53 pubmed
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图像
图像 1 :
Immunoprecipitation of HIF-1alpha using MA1-46457; Heavy and light chains are also detected.
图像 2 :
Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-46457.
图像 3 :
On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.
产品信息
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A单克隆抗体(H1alpha67), 生物素
目录# :
MA1-46457
规格 :
100微升
价格 :
美国449.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Avian, Bovine, Canine, Ferret, Human, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat
应用 :
ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): 1:100-1:300, Western Blot: 1:500
物种 :
Avian, Bovine, Canine, Ferret, Human, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat
克隆 :
H1alpha67
抗体亚型 :
IgG2b
储存 :
4° C, store in dark
描述 :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
免疫原 :
融合蛋白含氨基酸432-528人的HIF-1alpha
格式 :
液体
应用w/稀释 :
ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): 1:100-1:300, Western Blot: 1:500
别名 :
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a
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公司信息
赛默飞世尔