产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
AHR单克隆抗体(RPT9)
目录 :
MA1-513
规格 :
100微升
价格 :
美国450.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
RPT9
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, EMSA, 染色质免疫沉淀 , 免疫组化-冰冻切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 28
出版应用/物种/样本/稀释参考文献
  • 免疫沉淀; 人类
Tsuji N, Fukuda K, Nagata Y, Okada H, Haga A, Hatakeyama S, et al. The activation mechanism of the aryl hydrocarbon receptor (AhR) by molecular chaperone HSP90. FEBS Open Bio. 2014;4:796-803 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100
Nakamura M, Ueda Y, Hayashi M, Kato H, Furuhashi T, Morita A. Tobacco smoke-induced skin pigmentation is mediated by the aryl hydrocarbon receptor. Exp Dermatol. 2013;22:556-8 pubmed 出版商
  • 免疫印迹; 人类
Fukushima K, Tsukimori K, Li D, Takao T, Morokuma S, Kato K, et al. Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines. Hum Exp Toxicol. 2012;31:550-6 pubmed 出版商
  • 免疫沉淀; 人类
Colombo F, Falvella F, Galvan A, Frullanti E, Kunitoh H, Ushijima T, et al. A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A. Mol Cancer. 2011;10:81 pubmed 出版商
  • 免疫组化; 小鼠; 1:100
Furumatsu K, Nishiumi S, Kawano Y, Ooi M, Yoshie T, Shiomi Y, et al. A role of the aryl hydrocarbon receptor in attenuation of colitis. Dig Dis Sci. 2011;56:2532-44 pubmed 出版商
  • EMSA; 大鼠
Wang X, Hawkins B, Miller D. Aryl hydrocarbon receptor-mediated up-regulation of ATP-driven xenobiotic efflux transporters at the blood-brain barrier. FASEB J. 2011;25:644-52 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Reyes Hernández O, Mejía García A, Sánchez Ocampo E, Cabañas Cortés M, Ramirez P, Chávez González L, et al. Ube2l3 gene expression is modulated by activation of the aryl hydrocarbon receptor: implications for p53 ubiquitination. Biochem Pharmacol. 2010;80:932-40 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Tan K, Wang B, Yang M, Boutros P, Macaulay J, Xu H, et al. Aryl hydrocarbon receptor is a transcriptional activator of the human breast cancer resistance protein (BCRP/ABCG2). Mol Pharmacol. 2010;78:175-85 pubmed 出版商
  • 免疫印迹; 人类
Iba M, Shin M, Caccavale R. Cytochromes P4501 (CYP1): catalytic activities and inducibility by diesel exhaust particle extract and benzo[a]pyrene in intact human lung ex vivo. Toxicology. 2010;273:35-44 pubmed 出版商
  • 免疫印迹; 人类
Watabe Y, Nazuka N, Tezuka M, Shimba S. Aryl hydrocarbon receptor functions as a potent coactivator of E2F1-dependent trascription activity. Biol Pharm Bull. 2010;33:389-97 pubmed
  • 免疫印迹; 小鼠
Nishiumi S, Yoshida M, Azuma T, Yoshida K, Ashida H. 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs an insulin signaling pathway through the induction of tumor necrosis factor-alpha in adipocytes. Toxicol Sci. 2010;115:482-91 pubmed 出版商
  • EMSA; 人类
Marchand A, Tomkiewicz C, Marchandeau J, Boitier E, Barouki R, Garlatti M. 2,3,7,8-Tetrachlorodibenzo-p-dioxin induces insulin-like growth factor binding protein-1 gene expression and counteracts the negative effect of insulin. Mol Pharmacol. 2005;67:444-52 pubmed
Liu L, Xu J, Xu X, Mao T, Niu W, Wu X, et al. Intestinal Stem Cells Damaged by Deoxycholic Acid via AHR Pathway Contributes to Mucosal Barrier Dysfunction in High-Fat Feeding Mice. Int J Mol Sci. 2022;23: pubmed 出版商
Sun H, Han W, Wen J, Ma X. IL4I1 and tryptophan metabolites enhance AHR signals to facilitate colorectal cancer progression and immunosuppression. Am J Transl Res. 2022;14:7758-7770 pubmed
Kim Y, Ko B, Kim D, Tak J, Han C, Cho J, et al. Induction of the hepatic aryl hydrocarbon receptor by alcohol dysregulates autophagy and phospholipid metabolism via PPP2R2D. Nat Commun. 2022;13:6080 pubmed 出版商
Xu L, Yuan Y, Yu H, Wang S, Wang P. LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis. J Transl Med. 2022;20:350 pubmed 出版商
van de Veerdonk F, Renga G, Pariano M, Bellet M, Servillo G, Fallarino F, et al. Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy. J Clin Invest. 2021;: pubmed 出版商
Desmet N, Dhusia K, Qi W, Doseff A, Bhattacharya S, Gilad A. Bioengineering of Genetically Encoded Gene Promoter Repressed by the Flavonoid Apigenin for Constructing Intracellular Sensor for Molecular Events. Biosensors (Basel). 2021;11: pubmed 出版商
Yang X, Liu H, Ye T, Duan C, Lv P, Wu X, et al. AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization. Theranostics. 2020;10:12011-12025 pubmed 出版商
Mohammadi S, Memarian A, Sedighi S, Behnampour N, Yazdani Y. Immunoregulatory effects of indole-3-carbinol on monocyte-derived macrophages in systemic lupus erythematosus: A crucial role for aryl hydrocarbon receptor. Autoimmunity. 2018;51:199-209 pubmed 出版商
Wang L, Zhai D, Ruan B, Xu C, Ye Z, Lu H, et al. Quaking Deficiency Amplifies Inflammation in Experimental Endotoxemia via the Aryl Hydrocarbon Receptor/Signal Transducer and Activator of Transcription 1-NF-κB Pathway. Front Immunol. 2017;8:1754 pubmed 出版商
Neuschäfer Rube F, Schraplau A, Schewe B, Lieske S, Krützfeldt J, Ringel S, et al. Arylhydrocarbon receptor-dependent mIndy (Slc13a5) induction as possible contributor to benzo[a]pyrene-induced lipid accumulation in hepatocytes. Toxicology. 2015;337:1-9 pubmed 出版商
Chen Y, Beischlag T, Kim J, Perdew G, Stallcup M. Role of GAC63 in transcriptional activation mediated by the aryl hydrocarbon receptor. J Biol Chem. 2006;281:12242-7 pubmed
Kim J, Stallcup M. Role of the coiled-coil coactivator (CoCoA) in aryl hydrocarbon receptor-mediated transcription. J Biol Chem. 2004;279:49842-8 pubmed
Gouédard C, Barouki R, Morel Y. Dietary polyphenols increase paraoxonase 1 gene expression by an aryl hydrocarbon receptor-dependent mechanism. Mol Cell Biol. 2004;24:5209-22 pubmed
Fukuda I, Nishiumi S, Yabushita Y, Mukai R, Kodoi R, Hashizume K, et al. A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists. J Immunol Methods. 2004;287:187-201 pubmed
Savouret J, Antenos M, Quesne M, Xu J, Milgrom E, Casper R. 7-ketocholesterol is an endogenous modulator for the arylhydrocarbon receptor. J Biol Chem. 2001;276:3054-9 pubmed
Meyer B, Perdew G. Characterization of the AhR-hsp90-XAP2 core complex and the role of the immunophilin-related protein XAP2 in AhR stabilization. Biochemistry. 1999;38:8907-17 pubmed
图像
图像 1 :
赛默飞世尔 MA1-513 图像 1
Western blot analysis of Aryl Hydrocarbon Receptor was performed by loading 40 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane) or empty vector control (left lane) and 10ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Aryl Hydrocarbon Receptor monoclonal antibody (Product # MA1-513) at a dilution of 1:1000 overnight at 4ºC on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).
图像 2 :
赛默飞世尔 MA1-513 图像 2
Immunofluorescent analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) (Product# MA1-513 ) shows staining in A2058 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor (Product# MA1-513 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 3 :
赛默飞世尔 MA1-513 图像 3
Immunofluorescent analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) (Product# MA1-513 ) shows staining in Hela Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor (Product# MA1-513 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
AHR单克隆抗体(RPT9)
目录# :
MA1-513
规格 :
100微升
价格 :
美国450.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
人类, 小鼠, 大鼠
应用 :
ChIP Assay: 1:100, Flow Cytometry: 1:50, Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000
物种 :
人类, 小鼠, 大鼠
克隆 :
RPT9
抗体亚型 :
IgG3, kappa
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.
免疫原 :
Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.
格式 :
液体
应用w/稀释 :
ChIP Assay: 1:100, Flow Cytometry: 1:50, Gel Shift: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000
别名 :
Ah; Ah receptor; Ahh; Ahr; Ahre; AH-receptor; aromatic hydrocarbon receptor; aryl hydrocarbon receptor; aryl-hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor; In
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国