产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A单克隆抗体(mgc3)
目录 :
MA1-516
规格 :
100µL
价格 :
美国381.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
mgc3
反应物种 :
人类, 小鼠, 大鼠, ,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, EMSA, 免疫组化-石蜡切片, 抑制或激活实验
更多信息或购买 :
文章摘录数: 23
出版应用/物种/样品/稀释参考文献
  • 免疫细胞化学; 小鼠; 1:200; 图 s1a
Omatsu Kanbe M, Nozuchi N, Nishino Y, Mukaisho K, Sugihara H, Matsuura H. Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death. Sci Rep. 2017;7:41318 pubmed 出版商
  • 免疫印迹; 人类; 图 1a
Higashimura Y, Kitakaze T, Harada N, Inui H, Nakano Y, Yamaji R. pVHL-mediated degradation of HIF-2? regulates estrogen receptor ? expression in normoxic breast cancer cells. FEBS Lett. 2016;590:2690-9 pubmed 出版商
  • EMSA; 大鼠
  • EMSA; 小鼠
Kulzer J, Stitzel M, Morken M, Huyghe J, Fuchsberger C, Kuusisto J, et al. A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell. Am J Hum Genet. 2014;94:186-97 pubmed 出版商
  • 免疫组化; 人类; 1:50
  • 免疫印迹; 人类; 1:2000
Mandl M, Kapeller B, Lieber R, Macfelda K. Hypoxia-inducible factor-1? (HIF-1?) is upregulated in a HIF-1?-dependent manner in 518A2 human melanoma cells under hypoxic conditions. Biochem Biophys Res Commun. 2013;434:166-72 pubmed 出版商
  • 免疫印迹; 大鼠; 1:1500
Ma Y, Lovekamp Swan T, Bekele W, Dohi A, Schreihofer D. Hypoxia-inducible factor and vascular endothelial growth factor are targets of dietary soy during acute stroke in female rats. Endocrinology. 2013;154:1589-97 pubmed 出版商
  • 抑制或激活实验; 小鼠
Jin J, Sison K, Li C, Tian R, Wnuk M, Sung H, et al. Soluble FLT1 binds lipid microdomains in podocytes to control cell morphology and glomerular barrier function. Cell. 2012;151:384-99 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500
Croci D, Salatino M, Rubinstein N, Cerliani J, Cavallin L, Leung H, et al. Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma. J Exp Med. 2012;209:1985-2000 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫组化; 人类
  • 免疫印迹; 人类
Mitani T, Harada N, Nakano Y, Inui H, Yamaji R. Coordinated action of hypoxia-inducible factor-1? and ?-catenin in androgen receptor signaling. J Biol Chem. 2012;287:33594-606 pubmed
  • 免疫印迹; 人类
Higashimura Y, Nakajima Y, Yamaji R, Harada N, Shibasaki F, Nakano Y, et al. Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells. Arch Biochem Biophys. 2011;509:1-8 pubmed 出版商
  • 免疫细胞化学; 人类; 1:500
  • 免疫印迹; 人类; 1:2000
Mitani T, Yamaji R, Higashimura Y, Harada N, Nakano Y, Inui H. Hypoxia enhances transcriptional activity of androgen receptor through hypoxia-inducible factor-1? in a low androgen environment. J Steroid Biochem Mol Biol. 2011;123:58-64 pubmed 出版商
  • 免疫印迹; 牛; 1:2,000
Xing D, Bonanno J. Hypoxia preconditioning protection of corneal stromal cells requires HIF1alpha but not VEGF. Mol Vis. 2009;15:1020-7 pubmed
  • 免疫印迹; 人类
Kokkonen N, Ulibarri I, Kauppila A, Luosujärvi H, Rivinoja A, Pospiech H, et al. Hypoxia upregulates carcinoembryonic antigen expression in cancer cells. Int J Cancer. 2007;121:2443-50 pubmed
  • 免疫沉淀; 人类
  • 免疫组化; 人类; 1:50
  • 免疫印迹; 人类; 1:250
Ietta F, Wu Y, Winter J, Xu J, Wang J, Post M, et al. Dynamic HIF1A regulation during human placental development. Biol Reprod. 2006;75:112-21 pubmed
  • 免疫印迹; 牛
Xing D, Sun X, Li J, Cui M, Tan Allen K, Bonanno J. Hypoxia preconditioning protects corneal stromal cells against induced apoptosis. Exp Eye Res. 2006;82:780-7 pubmed
  • 免疫印迹; 小鼠; 1:100
van Tuyl M, Liu J, Wang J, Kuliszewski M, Tibboel D, Post M. Role of oxygen and vascular development in epithelial branching morphogenesis of the developing mouse lung. Am J Physiol Lung Cell Mol Physiol. 2005;288:L167-78 pubmed
Rathman Josserand M, Genty G, Lecardonnel J, Chabane S, Cousson A, François Michelet J, et al. Human hair follicle stem/progenitor cells express hypoxia markers. J Invest Dermatol. 2013;133:2094-7 pubmed 出版商
Higashimura Y, Terai T, Yamaji R, Mitani T, Ogawa M, Harada N, et al. Kelch-like 20 up-regulates the expression of hypoxia-inducible factor-2? through hypoxia- and von Hippel-Lindau tumor suppressor protein-independent regulatory mechanisms. Biochem Biophys Res Commun. 2011;413:201-5 pubmed 出版商
Zamudio S, Wu Y, Ietta F, Rolfo A, Cross A, Wheeler T, et al. Human placental hypoxia-inducible factor-1alpha expression correlates with clinical outcomes in chronic hypoxia in vivo. Am J Pathol. 2007;170:2171-9 pubmed
Bayer I, Caniggia I, Adamson S, Langille B. Experimental angiogenesis of arterial vasa vasorum. Cell Tissue Res. 2002;307:303-13 pubmed
Fernandez A, Udagawa T, Schwesinger C, Beecken W, Achilles Gerte E, McDonnell T, et al. Angiogenic potential of prostate carcinoma cells overexpressing bcl-2. J Natl Cancer Inst. 2001;93:208-13 pubmed
Wanner R, Spielmann P, Stroka D, Camenisch G, Camenisch I, Scheid A, et al. Epolones induce erythropoietin expression via hypoxia-inducible factor-1 alpha activation. Blood. 2000;96:1558-65 pubmed
Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, et al. General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha. FASEB J. 1999;13:81-8 pubmed
Wenger R, Kvietikova I, Rolfs A, Camenisch G, Gassmann M. Oxygen-regulated erythropoietin gene expression is dependent on a CpG methylation-free hypoxia-inducible factor-1 DNA-binding site. Eur J Biochem. 1998;253:771-7 pubmed
图像
图像 1 :
赛默飞世尔 MA1-516 图像 1
Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in Hela Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 2 :
赛默飞世尔 MA1-516 图像 2
Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in U251 Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 3 :
赛默飞世尔 MA1-516 图像 3
Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing HIF-1 alpha (MA1-516) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A单克隆抗体(mgc3)
目录# :
MA1-516
规格 :
100µL
价格 :
美国381.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Bovine, Human, Mouse, Non-human primate, Porcine
应用 :
Gel Shift: Assay dependent, Immunocytochemistry: 1:20-1:200, Immunoelectrophoresis: Assay Dependent, Immunofluorescence: 1:100, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay dependent, Western Blot: 1:2000
物种 :
Bovine, Human, Mouse, Non-human primate, Porcine
克隆 :
mgc3
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
免疫原 :
人类HIF-1甲氨基酸530-826
格式 :
Liquid
应用w/稀释 :
Gel Shift: Assay dependent, Immunocytochemistry: 1:20-1:200, Immunoelectrophoresis: Assay Dependent, Immunofluorescence: 1:100, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay dependent, Western Blot: 1:2000
别名 :
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国