产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A单克隆抗体(mgc3)
目录 :
MA1-516
规格 :
100微升
价格 :
美国516.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
mgc3
反应物种 :
人类, 小鼠, 大鼠, 牛, pigs
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, EMSA, 免疫组化-石蜡切片, 抑制或激活实验
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文章摘录数: 37
| 出版应用/物种/样本/稀释 | 参考文献 |
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| Mitani T, Harada N, Nakano Y, Inui H, Yamaji R. Coordinated action of hypoxia-inducible factor-1? and ?-catenin in androgen receptor signaling. J Biol Chem. 2012;287:33594-606 pubmed
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| Xing D, Bonanno J. Hypoxia preconditioning protection of corneal stromal cells requires HIF1alpha but not VEGF. Mol Vis. 2009;15:1020-7 pubmed
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| Kokkonen N, Ulibarri I, Kauppila A, Luosujärvi H, Rivinoja A, Pospiech H, et al. Hypoxia upregulates carcinoembryonic antigen expression in cancer cells. Int J Cancer. 2007;121:2443-50 pubmed
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| Ietta F, Wu Y, Winter J, Xu J, Wang J, Post M, et al. Dynamic HIF1A regulation during human placental development. Biol Reprod. 2006;75:112-21 pubmed
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| Xing D, Sun X, Li J, Cui M, Tan Allen K, Bonanno J. Hypoxia preconditioning protects corneal stromal cells against induced apoptosis. Exp Eye Res. 2006;82:780-7 pubmed
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| van Tuyl M, Liu J, Wang J, Kuliszewski M, Tibboel D, Post M. Role of oxygen and vascular development in epithelial branching morphogenesis of the developing mouse lung. Am J Physiol Lung Cell Mol Physiol. 2005;288:L167-78 pubmed
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Zamudio S, Wu Y, Ietta F, Rolfo A, Cross A, Wheeler T, et al. Human placental hypoxia-inducible factor-1alpha expression correlates with clinical outcomes in chronic hypoxia in vivo. Am J Pathol. 2007;170:2171-9 pubmed
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Bayer I, Caniggia I, Adamson S, Langille B. Experimental angiogenesis of arterial vasa vasorum. Cell Tissue Res. 2002;307:303-13 pubmed
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Fernandez A, Udagawa T, Schwesinger C, Beecken W, Achilles Gerte E, McDonnell T, et al. Angiogenic potential of prostate carcinoma cells overexpressing bcl-2. J Natl Cancer Inst. 2001;93:208-13 pubmed
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Wanner R, Spielmann P, Stroka D, Camenisch G, Camenisch I, Scheid A, et al. Epolones induce erythropoietin expression via hypoxia-inducible factor-1 alpha activation. Blood. 2000;96:1558-65 pubmed
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Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, et al. General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha. FASEB J. 1999;13:81-8 pubmed
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Wenger R, Kvietikova I, Rolfs A, Camenisch G, Gassmann M. Oxygen-regulated erythropoietin gene expression is dependent on a CpG methylation-free hypoxia-inducible factor-1 DNA-binding site. Eur J Biochem. 1998;253:771-7 pubmed
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图像
图像 1 :
Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in Hela Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 2 :
Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in U251 Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 3 :
Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing HIF-1 alpha (MA1-516) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A单克隆抗体(mgc3)
目录# :
MA1-516
规格 :
100微升
价格 :
美国516.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Bovine, Human, Mouse, Non-human primate, Porcine
应用 :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:20-1:200, Immunoelectrophoresis: Assay-dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:2,000
物种 :
Bovine, Human, Mouse, Non-human primate, Porcine
克隆 :
mgc3
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
免疫原 :
人类HIF-1甲氨基酸530-826
格式 :
液体
应用w/稀释 :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:20-1:200, Immunoelectrophoresis: Assay-dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:2,000
别名 :
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a
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公司信息
赛默飞世尔