产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
SRC3单克隆抗体(AX15.3)
目录 :
MA1-845
规格 :
100微克
价格 :
美国450.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AX15.3
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫沉淀, 染色质免疫沉淀
, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 5
| 出版应用/物种/样本/稀释 | 参考文献 |
|---|---|
| |
| Naughton C, MacLeod K, Kuske B, Clarke R, Cameron D, Langdon S. Progressive loss of estrogen receptor alpha cofactor recruitment in endocrine resistance. Mol Endocrinol. 2007;21:2615-26 pubmed
|
Park K, Krishnan V, O Malley B, Yamamoto Y, Gaynor R. Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation. Mol Cell. 2005;18:71-82 pubmed
| |
Oda Y, Sihlbom C, Chalkley R, Huang L, Rachez C, Chang C, et al. Two distinct coactivators, DRIP/mediator and SRC/p160, are differentially involved in vitamin D receptor transactivation during keratinocyte differentiation. Mol Endocrinol. 2003;17:2329-39 pubmed
| |
Azorsa D, Meltzer P. Production and characterization of monoclonal antibodies to the steroid receptor coactivator AIB1. Hybridoma. 1999;18:281-7 pubmed
|
图像
图像 1 :
Western blot of AIB1 on MCF-7 cell extract using MA1-845.
图像 2 :
Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a SRC3 monoclonal antibody (Product # MA1-845) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting
产品信息
产品类型 :
抗体
产品名称 :
SRC3单克隆抗体(AX15.3)
目录# :
MA1-845
规格 :
100微克
价格 :
美国450.00
克隆性 :
单克隆
纯度 :
纯化
宿主 :
小鼠
反应物种 :
人类
应用 :
ChIP Assay: Assay-dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 2 µg/mL
物种 :
人类
克隆 :
AX15.3
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
AIB1-AC3 recognizes AIB1, a member of the SRC-1 family of nuclear receptor coactivators with an apparent molecular weight of 160kDa. The AIB1 gene has been mapped to region 20q12 and has been shown to be amplified in breast tumors and pancreatic cancer cell lines. AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation.
免疫原 :
GST fusion protein encoding amino acid residues 605-1294 of human AIB1.
格式 :
液体
应用w/稀释 :
ChIP Assay: Assay-dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 2 µg/mL
别名 :
2010305B15Rik; Actr; AIB1; AIB-1; amplified in breast cancer 1 protein; amplified in breast cancer-1 protein homolog; AW321064; BHLHE42; CAGH16; CBP-interacting protein; class E basic helix-loop-helix protein 42; CTG26; KAT13B; MGC141848; NCOA3; NCoA-3; Nuclear receptor coactivator 3; OTTHUMP00000031716; p/Cip; pCip; Rac3; RAC-3; Receptor-associated coactivator 3; RP5-1049G16.3; Src3; SRC-3; steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; Tram1; Tram-1
更多信息或购买 :
公司信息
赛默飞世尔