γ-微管单克隆抗体(4D11) | 赛默飞世尔 MA1-850 产品信息

产品简要

公司名称 :

赛默飞世尔

其他品牌 :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

产品类型 :

抗体

产品名称 :

γ-微管单克隆抗体(4D11)

MA1-850

规格 :

100微克

价格 :

345美元

克隆性 :

单克隆

宿主 :

小鼠

共轭标签 :

未共轭

克隆名称 :

4D11

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

更多信息或购买 :

赛默飞世尔产品网页

文章摘录数: 17

出版应用/物种/样本/稀释	参考文献
免疫印迹; 人类; 图 8a	Abu Taha I, Heijman J, Hippe H, Wolf N, El Armouche A, Nikolaev V, et al. Nucleoside Diphosphate Kinase-C Suppresses cAMP Formation in Human Heart Failure. Circulation. 2017;135:881-897 pubmed 出版商
免疫印迹; 人类; 图 1c	Maina P, Shao P, Liu Q, Fazli L, Tyler S, Nasir M, et al. c-MYC drives histone demethylase PHF8 during neuroendocrine differentiation and in castration-resistant prostate cancer. Oncotarget. 2016;7:75585-75602 pubmed 出版商
免疫细胞化学; 人类; 图 2	Acevedo Acevedo S, Crone W. Substrate stiffness effect and chromosome missegregation in hIPS cells. J Negat Results Biomed. 2015;14:22 pubmed 出版商
免疫印迹; 小鼠	Patrick P, Price T, Diogo A, Sheibani N, Banks W, Shah G. Topiramate Protects Pericytes from Glucotoxicity: Role for Mitochondrial CA VA in Cerebromicrovascular Disease in Diabetes. J Endocrinol Diabetes. 2015;2: pubmed
免疫印迹; 人类	Fuentes E, Forero Doria O, AlarcÃ³n M, Palomo I. Inhibitory effects of Cyperus digitatus extract on human platelet function in vitro. Platelets. 2015;26:764-70 pubmed 出版商
免疫印迹; 人类	Fuentes E, AlarcÃ³n M, Fuentes M, Carrasco G, Palomo I. A novel role of Eruca sativa Mill. (rocket) extract: antiplatelet (NF-ÎºB inhibition) and antithrombotic activities. Nutrients. 2014;6:5839-52 pubmed 出版商
免疫印迹; 人类	Yan S, Wang C, Wei W, Gaertig M, Lai L, Li S, et al. TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain. Hum Mol Genet. 2014;23:2678-93 pubmed 出版商
免疫组化; 小鼠	Smith W, Liu Z, Liang Y, Masuda N, Swing D, Jenkins N, et al. Synphilin-1 attenuates neuronal degeneration in the A53T alpha-synuclein transgenic mouse model. Hum Mol Genet. 2010;19:2087-98 pubmed 出版商
免疫细胞化学; 人类免疫印迹; 人类	Seitzer U, Gerber S, Beyer D, Dobschanski J, Kullmann B, Haller D, et al. Schizonts of Theileria annulata interact with the microtubuli network of their host cell via the membrane protein TaSP. Parasitol Res. 2010;106:1085-102 pubmed 出版商
	Liu Q, Borcherding N, Shao P, Maina P, Zhang W, Qi H. Contribution of synergism between PHF8 and HER2 signalling to breast cancer development and drug resistance. EBioMedicine. 2020;51:102612 pubmed 出版商
	Liu Q, Borcherding N, Shao P, Cao H, Zhang W, Qi H. Identification of novel TGF-β regulated genes with pro-migratory roles. Biochim Biophys Acta Mol Basis Dis. 2019;1865:165537 pubmed 出版商
	Groenendyk J, Paskevicius T, Urra H, Viricel C, Wang K, Barakat K, et al. Cyclosporine A binding to COX-2 reveals a novel signaling pathway that activates the IRE1α unfolded protein response sensor. Sci Rep. 2018;8:16678 pubmed 出版商
	Ohmine S, Salisbury J, Ingle J, Pettinato G, Haddox C, Haddad T, et al. Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells. Oncol Rep. 2018;39:1725-1730 pubmed 出版商
	Wang W, Liu W, Durnaoglu S, Lee S, Lian J, Lehner R, et al. Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis. Sci Rep. 2017;7:5941 pubmed 出版商
	Siriwardana N, Meyer R, Panchenko M. The novel function of JADE1S in cytokinesis of epithelial cells. Cell Cycle. 2015;14:2821-34 pubmed 出版商
	Zhou Z, Patel M, Ng N, Hsieh M, Orth A, Walker J, et al. Identification of synthetic lethality of PRKDC in MYC-dependent human cancers by pooled shRNA screening. BMC Cancer. 2014;14:944 pubmed 出版商
	Messaed C, Akoury E, Djuric U, Zeng J, Saleh M, Gilbert L, et al. NLRP7, a nucleotide oligomerization domain-like receptor protein, is required for normal cytokine secretion and co-localizes with Golgi and the microtubule-organizing center. J Biol Chem. 2011;286:43313-23 pubmed 出版商

图像

图像 1 :

Immunofluorescent analysis of g-Tubulin using anti-g-Tubulin monoclonal antibody (Pierce Product# MA1-850, Clone 4D11) (shown in green) in HeLa (A) and U2-OS cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Pierce Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing g-Tubulin (Pierce Product# MA1-850), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Pierce Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-547 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye (Pierce Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

图像 2 :

Western blot analysis of ?-Tubulin was performed by loading 50ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing g-Tubulin (Pierce Product # MA1-850, Clone 4D11) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Pierce Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Pierce Product #34075).

产品信息

产品类型 :

抗体

产品名称 :

γ-微管单克隆抗体(4D11)

目录# :

MA1-850

规格 :

100微克

价格 :

345美元

克隆性 :

单克隆

纯度 :

蛋白质G

宿主 :

小鼠

反应物种 :

Human, Mouse, Non-human primate, Rat

应用 :

Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry: 1:200, Immunoprecipitation: Assay dependent, Western Blot: 1:1000

物种 :

Human, Mouse, Non-human primate, Rat

克隆 :

4D11

抗体亚型 :

IgG2b

储存 :

-20° C, 避免反复冷冻/解冻

描述 :

This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.

免疫原 :

Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.

格式 :

液体

应用w/稀释 :

Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry: 1:200, Immunoprecipitation: Assay dependent, Western Blot: 1:1000

别名 :

1500010O08Rik; AI451582; AI503389; AI504772; CDCBM4; centrosome marker; gamma tubulin; gamma-1-tubulin; Gamma-2-tubulin; gamma-tubulin; gamma-tubulin complex component 1; GCP-1; similar to tubulin, gamma 1; TUBG; Tubg1; tubg1.L; TUBG2; TUBGCP1; Tubgl; tubulin gamma 1; tubulin gamma 1 L homeolog; tubulin gamma 2; Tubulin gamma-1 chain; Tubulin gamma-2 chain; tubulin, gamma 1; tubulin, gamma 2; tubulin, gamma polypeptide; Unknown (protein for MGC:133882); XELAEV_18002398mg; xgam; zgc:55894

更多信息或购买 :

赛默飞世尔产品网页

公司信息

赛默飞世尔

上海浦东新金桥路27号7号楼

analyze.cn@thermofisher.com

http://www.thermo.com.cn

1084193588

公司总部: 美国

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