产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
PMCA4 ATPase单克隆抗体(JA9)
目录 :
MA1-914
规格 :
100微克
价格 :
美国399.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
JA9
反应物种 :
人类, 小鼠, 大鼠, 牛, pigs , domestic rabbit
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 20
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类
Ho P, Pang S, Li M, Tse Z, Kung M, Sham P, et al. PMCA4 (ATP2B4) mutation in familial spastic paraplegia causes delay in intracellular calcium extrusion. Brain Behav. 2015;5:e00321 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:200
  • 免疫印迹; 小鼠; 1:500
Yang Y, Lee J, Jo H, Park S, Chang I, Muallem S, et al. Homer2 protein regulates plasma membrane Ca²⁺-ATPase-mediated Ca²⁺ signaling in mouse parotid gland acinar cells. J Biol Chem. 2014;289:24971-9 pubmed 出版商
  • 免疫组化; 牛; 1:50
  • 免疫印迹; 牛; 1:1000
Schwarz A, Wennemuth G, Post H, Brandenburger T, Aumuller G, Wilhelm B. Vesicular transfer of membrane components to bovine epididymal spermatozoa. Cell Tissue Res. 2013;353:549-61 pubmed 出版商
  • 免疫印迹; 人类; 1:1000
Curry M, Luk N, Kenny P, Roberts Thomson S, Monteith G. Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells. J Biol Chem. 2012;287:28598-608 pubmed 出版商
  • 免疫组化; domestic rabbit; 1:1000
  • 免疫印迹; domestic rabbit; 1:2500
Talarico E. Plasma membrane calcium-ATPase isoform four distribution changes during corneal epithelial wound healing. Mol Vis. 2010;16:2259-72 pubmed
  • 免疫组化; 人类; 1:10
Dally S, Monceau V, Corvazier E, Bredoux R, Raies A, Bobe R, et al. Compartmentalized expression of three novel sarco/endoplasmic reticulum Ca2+ATPase 3 isoforms including the switch to ER stress, SERCA3f, in non-failing and failing human heart. Cell Calcium. 2009;45:144-54 pubmed 出版商
  • 免疫沉淀; 小鼠; 1 ug/mg
  • 免疫组化; 小鼠; 1:100
  • 免疫印迹; 小鼠
Baggaley E, McLarnon S, Demeter I, Varga G, Bruce J. Differential regulation of the apical plasma membrane Ca(2+) -ATPase by protein kinase A in parotid acinar cells. J Biol Chem. 2007;282:37678-93 pubmed
  • 免疫细胞化学; 大鼠; 1:300
Xiao J, Gong S, LeDoux M. Caytaxin deficiency disrupts signaling pathways in cerebellar cortex. Neuroscience. 2007;144:439-61 pubmed
  • 免疫细胞化学; 人类
Weeraratne S, Valentine M, Cusick M, Delay R, Van Houten J. Plasma membrane calcium pumps in mouse olfactory sensory neurons. Chem Senses. 2006;31:725-30 pubmed
Sritangos P, Pena Alarcon E, James A, Sultan A, Richardson D, Bruce J. Plasma Membrane Ca2+ ATPase Isoform 4 (PMCA4) Has an Important Role in Numerous Hallmarks of Pancreatic Cancer. Cancers (Basel). 2020;12: pubmed 出版商
Lisek M, Ferenc B, Studzian M, Pulaski L, Guo F, Zylinska L, et al. Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction. Front Cell Neurosci. 2017;11:181 pubmed 出版商
Tauber P, Aichinger B, Christ C, Stindl J, Rhayem Y, Beuschlein F, et al. Cellular Pathophysiology of an Adrenal Adenoma-Associated Mutant of the Plasma Membrane Ca(2+)-ATPase ATP2B3. Endocrinology. 2016;157:2489-99 pubmed 出版商
Kruger W, Yun C, Monteith G, Poronnik P. Muscarinic-induced recruitment of plasma membrane Ca2+-ATPase involves PSD-95/Dlg/Zo-1-mediated interactions. J Biol Chem. 2009;284:1820-30 pubmed 出版商
Sepulveda M, Berrocal Carrillo M, Gasset M, Mata A. The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes. J Biol Chem. 2006;281:447-53 pubmed
Talarico E, Kennedy B, Marfurt C, Loeffler K, Mangini N. Expression and immunolocalization of plasma membrane calcium ATPase isoforms in human corneal epithelium. Mol Vis. 2005;11:169-78 pubmed
Krizaj D, Liu X, Copenhagen D. Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum). J Comp Neurol. 2004;475:463-80 pubmed
Tamiya S, Dean W, Paterson C, Delamere N. Regional distribution of Na,K-ATPase activity in porcine lens epithelium. Invest Ophthalmol Vis Sci. 2003;44:4395-9 pubmed
Filoteo A, Elwess N, Enyedi A, Caride A, Aung H, Penniston J. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem. 1997;272:23741-7 pubmed
Caride A, Filoteo A, Enyedi A, Verma A, Penniston J. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J. 1996;316 ( Pt 1):353-9 pubmed
Adamo H, Caride A, Penniston J. Use of expression mutants and monoclonal antibodies to map the erythrocyte Ca2+ pump. J Biol Chem. 1992;267:14244-9 pubmed
图像
图像 1 :
赛默飞世尔 MA1-914 图像 1
Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing PMCA4 ATPase (MA1-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
图像 2 :
赛默飞世尔 MA1-914 图像 2
图像 3 :
赛默飞世尔 MA1-914 图像 3
产品信息
产品类型 :
抗体
产品名称 :
PMCA4 ATPase单克隆抗体(JA9)
目录# :
MA1-914
规格 :
100微克
价格 :
美国399.00
克隆性 :
单克隆
纯度 :
纯化
宿主 :
小鼠
反应物种 :
牛, 人类, 猪, 大鼠
应用 :
ELISA: 1:15,000, Flow Cytometry: 2 µg / 10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 1:100-1:500, Immunohistochemistry (Paraffin): 1:100-1:500, Western Blot: 1:1,000
物种 :
牛, 人类, 猪, 大鼠
克隆 :
JA9
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin (Cat. # MA3-917 and MA3-918).
免疫原 :
纯化人类红细胞 PMCA
格式 :
液体
应用w/稀释 :
ELISA: 1:15,000, Flow Cytometry: 2 µg / 10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 1:100-1:500, Immunohistochemistry (Paraffin): 1:100-1:500, Western Blot: 1:1,000
别名 :
ATP2B2; ATP2B4; ATPase plasma membrane Ca2+ transporting 4; ATPase, Ca++ transporting, plasma membrane 4; ATPase, Ca++ transporting, plasma membrane 4 ATP synthase, H+ transporting, mitochondrial F1; ATPase, Ca++ transporting, plasma membrane 4 tv2; matrix-remodeling-associated protein 1; MXRA1; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; plasma membrane calcium ATPase 4; plasma membrane calcium ATPase 4 variant b; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium pump; Plasma membrane calcium pump isoform 4; plasma membrane calcium-pumping ATP-ase; plasma membrane calcium-transporting ATPase 4; PMCA4; PMCA4b; PMCA4x; sarcolemmal calcium pump
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国