产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
c Myc单克隆抗体(9E10)
目录 :
MA1-980
规格 :
100微克
价格 :
美国325.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
9E10
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 染色质免疫沉淀 , 免疫组化-石蜡切片, 免疫组化-冰冻切片, 核糖核酸免疫沉淀
更多信息或购买 :
文章摘录数: 171
参考文献
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图像
图像 1 :
赛默飞世尔 MA1-980 图像 1
Western blot analysis of c-Myc was performed by loading 40ug of HeLa or NCCIT nuclear fractions (NF) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with c-Myc Monoclonal Antibody, 9E10 (Product # MA1-980) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgG HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075). Nuclear fractions were generated using the NE-PER kit (Product # 78833).
产品信息
产品类型 :
抗体
产品名称 :
c Myc单克隆抗体(9E10)
目录# :
MA1-980
规格 :
100微克
价格 :
美国325.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
人类
应用 :
ChIP assay: Assay-dependent, ELISA: Assay-dependent, Flow Cytometry: 1:50-1:200, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Frozen): 1:50-1:1,000, Immunohistochemistry (Paraffin): 1:50-1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000
物种 :
人类
克隆 :
9E10
抗体亚型 :
IgG1
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.
免疫原 :
合成肽A(408)E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438)人的c Myc
格式 :
液体
应用w/稀释 :
ChIP assay: Assay-dependent, ELISA: Assay-dependent, Flow Cytometry: 1:50-1:200, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Frozen): 1:50-1:1,000, Immunohistochemistry (Paraffin): 1:50-1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000
别名 :
AU016757; Avian myelocytomatosis viral (v-myc) oncogene homolog; avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; class E basic helix-loop-helix protein 39; cmyc; c-Myc; c-myc epitope tag; c-myc proto-oncogene; mMyc; MRTL; Myc; Myc protein; Myc proto-oncogene protein; MYC proto-oncogene, bHLH transcription factor; Myc2; MYCC; myc-related translation/localization regulatory factor; myelocytomatosis oncogene; myelocytomatosis viral oncogene homolog; Niard; Nird; Proto-oncogene c-Myc; RNCMYC; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene-like protein
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国