产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
VCP单克隆抗体(5)
目录 :
MA3-004
规格 :
100微克
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5
反应物种 :
酵母菌目, 人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 26
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 图 4a
Matějů D, Franzmann T, Patel A, Kopach A, Boczek E, Maharana S, et al. An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function. EMBO J. 2017;36:1669-1687 pubmed 出版商
  • 免疫印迹; 人类; 图 2
Koizumi S, Irie T, Hirayama S, Sakurai Y, Yashiroda H, Naguro I, et al. The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction. elife. 2016;5: pubmed 出版商
  • 免疫印迹; 人类; 图 1b
Wang T, Xu W, Qin M, Yang Y, Bao P, Shen F, et al. Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response. J Biol Chem. 2016;291:14373-84 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:2000; 图 s2
  • 免疫印迹; 小鼠; 1:5000; 图 1
Rodriguez Ortiz C, Flores J, Valenzuela J, Rodriguez G, Zumkehr J, Tran D, et al. The Myoblast C2C12 Transfected with Mutant Valosin-Containing Protein Exhibits Delayed Stress Granule Resolution on Oxidative Stress. Am J Pathol. 2016;186:1623-34 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:5000; 表 1
Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology. 2016;36:441-447 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:5,000
Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
  • 免疫组化; 小鼠; 图 6
Kuijpers M, van Dis V, Haasdijk E, Harterink M, Vocking K, Post J, et al. Amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions represent an ER quality control compartment. Acta Neuropathol Commun. 2013;1:24 pubmed 出版商
  • 免疫印迹; 酵母菌目; 1:3000
Buchan J, Kolaitis R, Taylor J, Parker R. Eukaryotic stress granules are cleared by autophagy and Cdc48/VCP function. Cell. 2013;153:1461-74 pubmed 出版商
  • 免疫组化; 人类
Mori F, Tanji K, Toyoshima Y, Sasaki H, Yoshida M, Kakita A, et al. Valosin-containing protein immunoreactivity in tauopathies, synucleinopathies, polyglutamine diseases and intranuclear inclusion body disease. Neuropathology. 2013;33:637-44 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫组化; 人类; 1:2000
Rodriguez Ortiz C, Hoshino H, Cheng D, Liu Yescevitz L, Blurton Jones M, Wolozin B, et al. Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice. Am J Pathol. 2013;183:504-15 pubmed 出版商
  • 免疫印迹; 人类; 1:3000
Kim N, Tresse E, Kolaitis R, Molliex A, Thomas R, Alami N, et al. VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. Neuron. 2013;78:65-80 pubmed 出版商
  • 免疫组化; 人类
Hackman P, Sarparanta J, Lehtinen S, Vihola A, Evilä A, Jonson P, et al. Welander distal myopathy is caused by a mutation in the RNA-binding protein TIA1. Ann Neurol. 2013;73:500-9 pubmed 出版商
  • 免疫组化; 大鼠; 1:500
  • 免疫印迹; 大鼠; 1:10000
Cayli S, Ocakli S, Erdemir F, Tas U, Aslan H, Yener T, et al. Developmental expression of p97/VCP (Valosin-containing protein) and Jab1/CSN5 in the rat testis and epididymis. Reprod Biol Endocrinol. 2011;9:117 pubmed 出版商
  • 免疫印迹; 人类
Iida Y, Fujimori T, Okawa K, Nagata K, Wada I, Hosokawa N. SEL1L protein critically determines the stability of the HRD1-SEL1L endoplasmic reticulum-associated degradation (ERAD) complex to optimize the degradation kinetics of ERAD substrates. J Biol Chem. 2011;286:16929-39 pubmed 出版商
  • 免疫印迹; 人类
Ballar P, Pabuccuoglu A, Kose F. Different p97/VCP complexes function in retrotranslocation step of mammalian ER-associated degradation (ERAD). Int J Biochem Cell Biol. 2011;43:613-21 pubmed 出版商
  • 免疫印迹; 小鼠
  • 免疫细胞化学; 人类
Tresse E, Salomons F, Vesa J, Bott L, Kimonis V, Yao T, et al. VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD. Autophagy. 2010;6:217-27 pubmed
  • 免疫印迹; 人类
Ballar P, Ors A, Yang H, Fang S. Differential regulation of CFTRDeltaF508 degradation by ubiquitin ligases gp78 and Hrd1. Int J Biochem Cell Biol. 2010;42:167-73 pubmed 出版商
  • 免疫组化; 人类; 1:50000
Greenberg S, Watts G, Kimonis V, Amato A, Pinkus J. Nuclear localization of valosin-containing protein in normal muscle and muscle affected by inclusion-body myositis. Muscle Nerve. 2007;36:447-54 pubmed
  • 酶联免疫吸附测定; 人类
Saffar A, Alphonse M, Shan L, Hayglass K, Simons F, Gounni A. IgE modulates neutrophil survival in asthma: role of mitochondrial pathway. J Immunol. 2007;178:2535-41 pubmed
Wu X, Spence J, Das T, Yuan X, Chen C, Zhang Y, et al. Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. Cell Chem Biol. 2020;27:571-585.e6 pubmed 出版商
Wang B, Maxwell B, Joo J, Gwon Y, Messing J, Mishra A, et al. ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Mol Cell. 2019;74:742-757.e8 pubmed 出版商
Palomo G, Granatiero V, Kawamata H, Konràd C, Kim M, Arreguin A, et al. Parkin is a disease modifier in the mutant SOD1 mouse model of ALS. EMBO Mol Med. 2018;10: pubmed 出版商
Bento A, Bippes C, Kohler C, Hemion C, Frank S, Neutzner A. UBXD1 is a mitochondrial recruitment factor for p97/VCP and promotes mitophagy. Sci Rep. 2018;8:12415 pubmed 出版商
Ash P, Stanford E, Al Abdulatif A, Ramirez Cardenas A, Ballance H, Boudeau S, et al. Dioxins and related environmental contaminants increase TDP-43 levels. Mol Neurodegener. 2017;12:35 pubmed 出版商
Auranen M, Palmio J, Ylikallio E, Huovinen S, Paetau A, Sandell S, et al. PFKM gene defect and glycogen storage disease GSDVII with misleading enzyme histochemistry. Neurol Genet. 2015;1:e7 pubmed 出版商
Vij N, Fang S, Zeitlin P. Selective inhibition of endoplasmic reticulum-associated degradation rescues DeltaF508-cystic fibrosis transmembrane regulator and suppresses interleukin-8 levels: therapeutic implications. J Biol Chem. 2006;281:17369-78 pubmed
图像
图像 1 :
赛默飞世尔 MA3-004 图像 1
Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) (Product# MA3-004) shows staining in HeLa cells. VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP (Product# MA3-004) at a dilution of 1:20-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
VCP单克隆抗体(5)
目录# :
MA3-004
规格 :
100微克
价格 :
美国425.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
人类, 小鼠, 大鼠
应用 :
Flow Cytometry: 1/400, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
物种 :
人类, 小鼠, 大鼠
克隆 :
5
抗体亚型 :
IgG2a
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
免疫原 :
Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP.
格式 :
液体
应用w/稀释 :
Flow Cytometry: 1/400, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
别名 :
15S Mg(2+)-ATPase p97 subunit; 3110001E05; AAA ATPase p97; ALS14; CDC48; cell division cycle gene CDC48; CMT2Y; epididymis luminal protein 220; epididymis secretory protein Li 70; HEL-220; HEL-S-70; homolog of yeast cdc48; IBMPFD; IBMPFD1; Inv protein; or HEL-S-70; p97; p97 atpase; p97/VCP; Protein CDC48; si:ch211-113n10.2; TER ATPase; TERA; transitional endoplasmic reticulum ATPase; valosin containing protein; valosin-containing protein; Vcp; wu:fd16d05; wu:fj63d11; yeast Cdc48p homolog
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国