产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
HSP70单克隆抗体(3A3)
目录 :
MA3-006
规格 :
50微克
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3A3
反应物种 :
雪跳蚤, lobsters, 日本大米鱼, Artemia franciscana, Eyprepocnemis plorans, Pacific oyster, 酵母菌目, Marenzelleria viridis, red rock crab, sea monkeys, 衣藻, eastern oyster, common limpet, Asian green mussel, giant tiger prawn, crucian carp, Meganyctiphanes norvegica, holothurians, roach minnow, 人类, 小鼠, 大鼠, 犬, pigs , fruit fly , roundworm , 猕猴
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 抑制或激活实验
更多信息或购买 :
文章摘录数: 84
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| Oikari A, Lahti M, Meriläinen P, Afanasyev S, Krasnov A. Do historical sediments of pulp and paper industry contribute to the exposure of fish caged in receiving waters?. J Environ Monit. 2010;12:1045-54 pubmed
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| Li Y, Qin J, Abbott C, Li X, Benkendorff K. Synergistic impacts of heat shock and spawning on the physiology and immune health of Crassostrea gigas: an explanation for summer mortality in Pacific oysters. Am J Physiol Regul Integr Comp Physiol. 2007;293:R2353-62 pubmed
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| Encomio V, Chu F. Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus. Dis Aquat Organ. 2007;76:251-60 pubmed
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| Vianna C, Silva C, Neves M, Rosa C. Saccharomyces cerevisiae strains from traditional fermentations of Brazilian cachaça: trehalose metabolism, heat and ethanol resistance. Antonie Van Leeuwenhoek. 2008;93:205-17 pubmed
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| Silvestre F, Trausch G, Devos P. Is HSP70 involved in acclimation to cadmium in the Chinese crab, Eriocheir sinensis ?. Bull Environ Contam Toxicol. 2007;78:432-5 pubmed
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| Yik Sung Y, Van Damme E, Sorgeloos P, Bossier P. Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios. Fish Shellfish Immunol. 2007;22:318-26 pubmed
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| Blank M, Bastrop R, Jürss K. Stress protein response in two sibling species of Marenzelleria (Polychaeta: Spionidae): is there an influence of acclimation salinity?. Comp Biochem Physiol B Biochem Mol Biol. 2006;144:451-62 pubmed
|
| Mayon N, Bertrand A, Leroy D, Malbrouck C, Mandiki S, Silvestre F, et al. Multiscale approach of fish responses to different types of environmental contaminations: a case study. Sci Total Environ. 2006;367:715-31 pubmed
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| Neal S, Karunanithi S, Best A, So A, Tanguay R, Atwood H, et al. Thermoprotection of synaptic transmission in a Drosophila heat shock factor mutant is accompanied by increased expression of Hsp83 and DnaJ-1. Physiol Genomics. 2006;25:493-501 pubmed
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| Rissanen E, Tranberg H, Sollid J, Nilsson G, Nikinmaa M. Temperature regulates hypoxia-inducible factor-1 (HIF-1) in a poikilothermic vertebrate, crucian carp (Carassius carassius). J Exp Biol. 2006;209:994-1003 pubmed
|
| Krauss M, Haucke V. Functional assay of effectors of ADP ribosylation factor 6 during clathrin/AP-2 coat recruitment to membranes. Methods Enzymol. 2005;404:388-98 pubmed
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| Yang C, Compton M, Yang P. Dimeric novel HSP40 is incorporated into the radial spoke complex during the assembly process in flagella. Mol Biol Cell. 2005;16:637-48 pubmed
|
Schmitz M, Douxfils J, Mandiki S, Morana C, Baekelandt S, Kestemont P. Chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (Pangasianodon hypophthalmus, S.) and leads to excessive inflammatory response during bacterial infection. Fish Shellfish Immunol. 2016;55:550-8 pubmed 出版商
| |
Shapiro J, Ingram J, Johnson K. Characterization of a molecular chaperone present in the eukaryotic flagellum. Eukaryot Cell. 2005;4:1591-4 pubmed
| |
Mitchell B, Pedersen L, Feely M, Rosenbaum J, Mitchell D. ATP production in Chlamydomonas reinhardtii flagella by glycolytic enzymes. Mol Biol Cell. 2005;16:4509-18 pubmed
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Häkkinen J, Oikari A. A field methodology to study effects of UV radiation on fish larvae. Water Res. 2004;38:2891-7 pubmed
| |
Häkkinen J, Vehniäinen E, Oikari A. High sensitivity of northern pike larvae to UV-B but no UV-photoinduced toxicity of retene. Aquat Toxicol. 2004;66:393-404 pubmed
| |
Stavreva D, Müller W, Hager G, Smith C, McNally J. Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes. Mol Cell Biol. 2004;24:2682-97 pubmed
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Sashchenko L, Dukhanina E, Yashin D, Shatalov Y, Romanova E, Korobko E, et al. Peptidoglycan recognition protein tag7 forms a cytotoxic complex with heat shock protein 70 in solution and in lymphocytes. J Biol Chem. 2004;279:2117-24 pubmed
| |
Wang G, Zhang J, Moskophidis D, Mivechi N. Targeted disruption of the heat shock transcription factor (hsf)-2 gene results in increased embryonic lethality, neuronal defects, and reduced spermatogenesis. Genesis. 2003;36:48-61 pubmed
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Cruz Rodríguez L, Chu F. Heat-shock protein (HSP70) response in the eastern oyster, Crassostrea virginica, exposed to PAHs sorbed to suspended artificial clay particles and to suspended field contaminated sediments. Aquat Toxicol. 2002;60:157-68 pubmed
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Lafarga M, Berciano M, Pena E, Mayo I, Castaño J, Bohmann D, et al. Clastosome: a subtype of nuclear body enriched in 19S and 20S proteasomes, ubiquitin, and protein substrates of proteasome. Mol Biol Cell. 2002;13:2771-82 pubmed
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Cimino E, Owens L, Bromage E, Anderson T. A newly developed ELISA showing the effect of environmental stress on levels of hsp86 in Cherax quadricarinatus and Penaeus monodon. Comp Biochem Physiol A Mol Integr Physiol. 2002;132:591-8 pubmed
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Huang L, Mivechi N, Moskophidis D. Insights into regulation and function of the major stress-induced hsp70 molecular chaperone in vivo: analysis of mice with targeted gene disruption of the hsp70.1 or hsp70.3 gene. Mol Cell Biol. 2001;21:8575-91 pubmed
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King F, Wawrzynow A, Hohfeld J, Zylicz M. Co-chaperones Bag-1, Hop and Hsp40 regulate Hsc70 and Hsp90 interactions with wild-type or mutant p53. EMBO J. 2001;20:6297-305 pubmed
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Fishelson Z, Hochman I, Greene L, Eisenberg E. Contribution of heat shock proteins to cell protection from complement-mediated lysis. Int Immunol. 2001;13:983-91 pubmed
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Hynes G, Willison K. Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin. J Biol Chem. 2000;275:18985-94 pubmed
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Bharadwaj S, Ali A, Ovsenek N. Multiple components of the HSP90 chaperone complex function in regulation of heat shock factor 1 In vivo. Mol Cell Biol. 1999;19:8033-41 pubmed
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Ballinger C, Connell P, Wu Y, Hu Z, Thompson L, Yin L, et al. Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions. Mol Cell Biol. 1999;19:4535-45 pubmed
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Bierkens J, Van de Perre W, Maes J. Effect of different environmental variables on the synthesis of Hsp70 in Raphidocelis subcapitata. Comp Biochem Physiol A Mol Integr Physiol. 1998;120:29-34 pubmed
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Mathew A, Mathur S, Morimoto R. Heat shock response and protein degradation: regulation of HSF2 by the ubiquitin-proteasome pathway. Mol Cell Biol. 1998;18:5091-8 pubmed
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Anton L, Snyder H, Bennink J, Vinitsky A, Orlowski M, Porgador A, et al. Dissociation of proteasomal degradation of biosynthesized viral proteins from generation of MHC class I-associated antigenic peptides. J Immunol. 1998;160:4859-68 pubmed
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Botzler C, Li G, Issels R, Multhoff G. Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response. Cell Stress Chaperones. 1998;3:6-11 pubmed
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Stephens R. Synthesis and turnover of embryonic sea urchin ciliary proteins during selective inhibition of tubulin synthesis and assembly. Mol Biol Cell. 1997;8:2187-98 pubmed
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Fourie A, Hupp T, Lane D, Sang B, Barbosa M, Sambrook J, et al. HSP70 binding sites in the tumor suppressor protein p53. J Biol Chem. 1997;272:19471-9 pubmed
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Piperno G, Mead K. Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella. Proc Natl Acad Sci U S A. 1997;94:4457-62 pubmed
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Bloch M, Johnson K. Identification of a molecular chaperone in the eukaryotic flagellum and its localization to the site of microtubule assembly. J Cell Sci. 1995;108 ( Pt 11):3541-5 pubmed
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Nihei T, Takahashi S, Sagae S, Sato N, Kikuchi K. Protein interaction of retinoblastoma gene product pRb110 with M(r) 73,000 heat shock cognate protein. Cancer Res. 1993;53:1702-5 pubmed
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Sarge K, Murphy S, Morimoto R. Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress. Mol Cell Biol. 1993;13:1392-407 pubmed
| |
Agoff S, Hou J, Linzer D, Wu B. Regulation of the human hsp70 promoter by p53. Science. 1993;259:84-7 pubmed
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Lithgow T, Ryan M, Anderson R, Høj P, Hoogenraad N. A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver. FEBS Lett. 1993;332:277-81 pubmed
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van Laack R, Faustman C, Sebranek J. Pork quality and the expression of stress protein Hsp 70 in swine. J Anim Sci. 1993;71:2958-64 pubmed
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Srinivasan G, Patel N, Thompson E. Heat shock protein is tightly associated with the recombinant human glucocorticoid receptor:glucocorticoid response element complex. Mol Endocrinol. 1994;8:189-96 pubmed
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Huang Q, Alvares K, Chu R, Bradfield C, Reddy J. Association of peroxisome proliferator-activated receptor and Hsp72. J Biol Chem. 1994;269:8493-7 pubmed
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Arnold C, Wittrup K. The stress response to loss of signal recognition particle function in Saccharomyces cerevisiae. J Biol Chem. 1994;269:30412-8 pubmed
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Rabindran S, Wisniewski J, Li L, Li G, Wu C. Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo. Mol Cell Biol. 1994;14:6552-60 pubmed
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Bhattacharyya T, Karnezis A, Murphy S, Hoang T, Freeman B, Phillips B, et al. Cloning and subcellular localization of human mitochondrial hsp70. J Biol Chem. 1995;270:1705-10 pubmed
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Carr V, Murphy S, Morimoto R, Farbman A. Small subclass of rat olfactory neurons with specific bulbar projections is reactive with monoclonal antibodies to the HSP70 heat shock protein. J Comp Neurol. 1994;348:150-60 pubmed
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Saitoh H, Dasso M. The RCC1 protein interacts with Ran, RanBP1, hsc70, and a 340-kDa protein in Xenopus extracts. J Biol Chem. 1995;270:10658-63 pubmed
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Inoue A, Torigoe T, Sogahata K, Kamiguchi K, Takahashi S, Sawada Y, et al. 70-kDa heat shock cognate protein interacts directly with the N-terminal region of the retinoblastoma gene product pRb. Identification of a novel region of pRb-mediating protein interaction. J Biol Chem. 1995;270:22571-6 pubmed
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Lewis V, Hynes G, Zheng D, Saibil H, Willison K. T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol. Nature. 1992;358:249-52 pubmed
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Nadler S, Tepper M, Schacter B, Mazzucco C. Interaction of the immunosuppressant deoxyspergualin with a member of the Hsp70 family of heat shock proteins. Science. 1992;258:484-6 pubmed
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图像
图像 1 :
Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) (Product# MA3-006) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-006) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 2 :
Western blot analysis of Heat Shock Protein 70 (Hsp70) was performed by loading 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp70 monoclonal antibody (Product # MA3-006) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
产品信息
产品类型 :
抗体
产品名称 :
HSP70单克隆抗体(3A3)
目录# :
MA3-006
规格 :
50微克
价格 :
美国425.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
Amphibian, Arthropod, Avian, Canine, Drosophila, Fish, Human, Mollusca, Mouse, Non-human primate, Plant, Porcine, Rat, Yeast
应用 :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:5,000
物种 :
Amphibian, Arthropod, Avian, Canine, Drosophila, Fish, Human, Mollusca, Mouse, Non-human primate, Plant, Porcine, Rat, Yeast
克隆 :
3A3
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
免疫原 :
Human recombinant HSP70 overexpressed in E. coli.
格式 :
液体
应用w/稀释 :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:5,000
别名 :
68 kDa heat shock protein; 70 kda heat shock protein-2; 70 kilodalton heat shock protein; 87A; 87A locus; 87C locus; Bb; CG18743; CG31359; CG31359-PA; CG31366; CG31366-PA; CG31449; CG6489; chaperone; dHSP70; dhsp70Aa; Dmel CG31359; Dmel CG31366; Dmel_CG31359; Dmel_CG31366; Dm-hsp70; DmHSP70AA; DMHSP7D1; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Hcp70.1; heat shock 10kDa protein 1-like; heat shock 70; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1L; heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70 kDa-like protein 1; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 1B (mapped); heat shock 70kD protein-like 1; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 1-like; heat shock cognate 70-kd protein, tandem duplicate 2; Heat shock protein; heat shock protein 1A; heat shock protein 1B; heat shock protein 1-like; heat shock protein 70; heat shock protein 70.1; heat shock protein 70.2; heat shock protein 70-1; Heat shock protein 70-1l; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein cognate 70, testis; heat shock protein family A (Hsp70) member 1 like; heat shock protein family A (Hsp70) member 1A; heat shock protein family A (Hsp70) member 1B; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; heat shock-related protein hsc70t; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; heat-shock protein 70; Heat-shock-protein-70Aa; Heat-shock-protein-70Bb; HEL S 103; HEL-S-103; hs70; Hsc70t; HSP; Hsp 70; hsp68; Hsp70; Hsp-70; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; hsp70 87C; hsp70 Aa; HSP70 AceyltK77; hsp70(87A); Hsp70(87C); HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; Hsp70.3; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; Hsp70-3; HSP70-87A7; HSP70-87C1; hsp70a; hsp70A1; Hsp70A7d; Hsp70Aa; Hsp-70Aa; Hsp70Aa-PA; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bb-PA; Hsp70Bc; HSP70-HOM; HSP70I; HSP70T; HSP72; Hspa1; Hspa1a; HSPA1B; Hspa1l; Hspa2; Hspa70; HSX70; hum70t; inducible heat shock protein 70; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; Msh5; protein 70; si:ch73-46h18.6; si:dkeyp-69e1.1; Spermatid-specific heat shock protein 70; zgc:174006
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公司信息
赛默飞世尔