产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
HSP70单克隆抗体(5A5)
目录 :
MA3-007
规格 :
250微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5A5
反应物种 :
African green monkey, scallops, California mussel, Pacific oyster, 仓鼠, Lycaena tityrus, forest day mosquito, 人类, 小鼠, 大鼠, 犬, fruit fly
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-冰冻切片, 抑制或激活实验
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 49
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; scallops; 图 7
Ivanina A, Nesmelova I, Leamy L, Sokolov E, Sokolova I. Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs. J Exp Biol. 2016;219:1659-74 pubmed 出版商
  • 免疫印迹; 人类; 图 1
Mutschelknaus L, Peters C, Winkler K, Yentrapalli R, Heider T, Atkinson M, et al. Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation. PLoS ONE. 2016;11:e0152213 pubmed 出版商
  • 免疫印迹; 小鼠
Azimzadeh O, Sievert W, Sarioglu H, Merl Pham J, Yentrapalli R, Bakshi M, et al. Integrative proteomics and targeted transcriptomics analyses in cardiac endothelial cells unravel mechanisms of long-term radiation-induced vascular dysfunction. J Proteome Res. 2015;14:1203-19 pubmed 出版商
  • 酶联免疫吸附测定; fruit fly ; 1:1000; 图 3
Calabria G, Dolgova O, Rego C, Castañeda L, Rezende E, Balanya J, et al. Hsp70 protein levels and thermotolerance in Drosophila subobscura: a reassessment of the thermal co-adaptation hypothesis. J Evol Biol. 2012;25:691-700 pubmed 出版商
  • 免疫印迹; 小鼠; 1:2000
Marshall D, Dong Y, McQuaid C, Williams G. Thermal adaptation in the intertidal snail Echinolittorina malaccana contradicts current theory by revealing the crucial roles of resting metabolism. J Exp Biol. 2011;214:3649-57 pubmed 出版商
  • 免疫印迹; 小鼠
Noack R, Frede S, Albrecht P, Henke N, Pfeiffer A, Knoll K, et al. Charcot-Marie-Tooth disease CMT4A: GDAP1 increases cellular glutathione and the mitochondrial membrane potential. Hum Mol Genet. 2012;21:150-62 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000
Bivi N, Picotti P, Müller L, Romanello M, Moro L, Quadrifoglio F, et al. Shotgun proteomics analysis reveals new unsuspected molecular effectors of nitrogen-containing bisphosphonates in osteocytes. J Proteomics. 2011;74:1113-22 pubmed 出版商
  • 酶联免疫吸附测定; Lycaena tityrus; 1:700; 图 2c
Fischer K, Kölzow N, Höltje H, Karl I. Assay conditions in laboratory experiments: is the use of constant rather than fluctuating temperatures justified when investigating temperature-induced plasticity?. Oecologia. 2011;166:23-33 pubmed 出版商
  • 免疫组化; fruit fly ; 1:200
Chen H, Anagnostou G, Chai A, Withers J, Morris A, Adhikaree J, et al. Characterization of the properties of a novel mutation in VAPB in familial amyotrophic lateral sclerosis. J Biol Chem. 2010;285:40266-81 pubmed 出版商
  • 免疫印迹; fruit fly
Juge F, Fernando C, Fic W, Tazi J. The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown. PLoS Genet. 2010;6:e1001124 pubmed 出版商
  • 免疫印迹; California mussel; 1:1000
Urian A, Hatle J, Gilg M. Thermal constraints for range expansion of the invasive green mussel, Perna viridis, in the southeastern United States. J Exp Zool A Ecol Genet Physiol. 2011;315:12-21 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:3000
Cho K, Yi H, Tserentsoodol N, Searle K, Ferreira P. Neuroprotection resulting from insufficiency of RANBP2 is associated with the modulation of protein and lipid homeostasis of functionally diverse but linked pathways in response to oxidative stress. Dis Model Mech. 2010;3:595-604 pubmed 出版商
  • 免疫印迹; 小鼠
Rozich R, Mills D, Brilliant K, Callanan H, Yang D, Tantravahi U, et al. Accumulation of neoplastic traits prior to spontaneous in vitro transformation of rat cholangiocytes determines susceptibility to activated ErbB-2/Neu. Exp Mol Pathol. 2010;89:248-59 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:50
Paoletti F, Ainger K, Donati I, Scardigli R, Vetere A, Cattaneo A, et al. Novel fluorescent cycloheximide derivatives for the imaging of protein synthesis. Biochem Biophys Res Commun. 2010;396:258-64 pubmed 出版商
  • 免疫组化; fruit fly ; 1:500
Nisoli I, Chauvin J, Napoletano F, Calamita P, Zanin V, Fanto M, et al. Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy. Cell Death Differ. 2010;17:1577-87 pubmed 出版商
  • 免疫印迹; 人类
Viteri G, Chung Y, Stadtman E. Effect of progerin on the accumulation of oxidized proteins in fibroblasts from Hutchinson Gilford progeria patients. Mech Ageing Dev. 2010;131:2-8 pubmed 出版商
  • 免疫印迹; 小鼠
Wong S, Leung P, Djurisić A, Leung K. Toxicities of nano zinc oxide to five marine organisms: influences of aggregate size and ion solubility. Anal Bioanal Chem. 2010;396:609-18 pubmed 出版商
  • 免疫印迹; 小鼠
Yuan X, Chen L, Li M, Dong P, Xue J, Wang J, et al. Elevated expression of Foxp3 in tumor-infiltrating Treg cells suppresses T-cell proliferation and contributes to gastric cancer progression in a COX-2-dependent manner. Clin Immunol. 2010;134:277-88 pubmed 出版商
  • 免疫组化; 人类; 1:150
  • 免疫印迹; 人类
Codarin E, Renzone G, Poz A, Avellini C, Baccarani U, Lupo F, et al. Differential proteomic analysis of subfractioned human hepatocellular carcinoma tissues. J Proteome Res. 2009;8:2273-84 pubmed 出版商
  • 免疫印迹; 小鼠
Tani F, Ohno M, Furukawa Y, Sakamoto M, Masuda S, Kitabatake N. Surface expression of a C-terminal alpha-helix region in heat shock protein 72 on murine LL/2 lung carcinoma can be recognized by innate immune sentinels. Mol Immunol. 2009;46:1326-39 pubmed 出版商
  • 免疫印迹; Pacific oyster
Ivanina A, Taylor C, Sokolova I. Effects of elevated temperature and cadmium exposure on stress protein response in eastern oysters Crassostrea virginica (Gmelin). Aquat Toxicol. 2009;91:245-54 pubmed 出版商
  • 流式细胞仪; 人类
  • 免疫印迹; 人类
Chavez Salinas S, Ceballos Olvera I, Reyes del Valle J, Medina F, Del Angel R. Heat shock effect upon dengue virus replication into U937 cells. Virus Res. 2008;138:111-8 pubmed 出版商
  • 免疫印迹; Pacific oyster
Ivanina A, Sokolova I, Sukhotin A. Oxidative stress and expression of chaperones in aging mollusks. Comp Biochem Physiol B Biochem Mol Biol. 2008;150:53-61 pubmed 出版商
  • 免疫印迹; Pacific oyster
Ivanina A, Cherkasov A, Sokolova I. Effects of cadmium on cellular protein and glutathione synthesis and expression of stress proteins in eastern oysters, Crassostrea virginica Gmelin. J Exp Biol. 2008;211:577-86 pubmed 出版商
  • 免疫印迹; 仓鼠; 1:5,000
  • 免疫印迹; forest day mosquito; 1:5,000
Salas Benito J, Reyes del Valle J, Salas Benito M, Ceballos Olvera I, Mosso C, Del Angel R. Evidence that the 45-kD glycoprotein, part of a putative dengue virus receptor complex in the mosquito cell line C6/36, is a heat-shock related protein. Am J Trop Med Hyg. 2007;77:283-90 pubmed
  • 抑制或激活实验; African green monkey; 1:100
  • 抑制或激活实验; 仓鼠; 1:100
López T, Lopez S, Arias C. Heat shock enhances the susceptibility of BHK cells to rotavirus infection through the facilitation of entry and post-entry virus replication steps. Virus Res. 2006;121:74-83 pubmed
  • 免疫组化; fruit fly ; 1:100
Chartier A, Benoit B, Simonelig M. A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1. EMBO J. 2006;25:2253-62 pubmed
  • 免疫印迹; 小鼠
Klose M, Chu D, Xiao C, Seroude L, Robertson R. Heat shock-mediated thermoprotection of larval locomotion compromised by ubiquitous overexpression of Hsp70 in Drosophila melanogaster. J Neurophysiol. 2005;94:3563-72 pubmed
  • 免疫印迹; fruit fly ; 1:1000
Newman A, Xiao C, Robertson R. Synaptic thermoprotection in a desert-dwelling Drosophila species. J Neurobiol. 2005;64:170-80 pubmed
Xiao C, Hull D, Qiu S, Yeung J, Zheng J, Barwell T, et al. Expression of Heat Shock Protein 70 Is Insufficient To Extend Drosophila melanogaster Longevity. G3 (Bethesda). 2019;9:4197-4207 pubmed 出版商
Attwood K, Fleyshman D, Prendergast L, Paszkiewicz G, Omilian A, Bshara W, et al. Prognostic value of histone chaperone FACT subunits expression in breast cancer. Breast Cancer (Dove Med Press). 2017;9:301-311 pubmed 出版商
Horikawa I, Fujita K, Jenkins L, Hiyoshi Y, Mondal A, Vojtesek B, et al. Autophagic degradation of the inhibitory p53 isoform ?133p53? as a regulatory mechanism for p53-mediated senescence. Nat Commun. 2014;5:4706 pubmed 出版商
Wong S, Leung K. Temperature-dependent toxicities of nano zinc oxide to marine diatom, amphipod and fish in relation to its aggregation size and ion dissolution. Nanotoxicology. 2014;8 Suppl 1:24-35 pubmed 出版商
Wang L, Baruah K, Fan T, Yu M, Bossier P. Influence of heat shock proteins induction in different yeast cell wall mutants on the protection against Vibrio campbellii infection in gnotobiotically grown Artemia franciscana (Kellogg). J Fish Dis. 2010;33:919-23 pubmed
Calvert M, Digilio L, Herr J, Coonrod S. Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane. Reprod Biol Endocrinol. 2003;1:27 pubmed
Fishelson Z, Hochman I, Greene L, Eisenberg E. Contribution of heat shock proteins to cell protection from complement-mediated lysis. Int Immunol. 2001;13:983-91 pubmed
Giraldo R, Diaz Orejas R. Similarities between the DNA replication initiators of Gram-negative bacteria plasmids (RepA) and eukaryotes (Orc4p)/archaea (Cdc6p). Proc Natl Acad Sci U S A. 2001;98:4938-43 pubmed
Thress K, Song J, Morimoto R, Kornbluth S. Reversible inhibition of Hsp70 chaperone function by Scythe and Reaper. EMBO J. 2001;20:1033-41 pubmed
de Haan P, Vanicky I, Jacobs M, Bakker O, Lips J, Meylaerts S, et al. Effect of ischemic pretreatment on heat shock protein 72, neurologic outcome, and histopathologic outcome in a rabbit model of spinal cord ischemia. J Thorac Cardiovasc Surg. 2000;120:513-9 pubmed
Bonner J, Carlson T, Fackenthal D, Paddock D, Storey K, Lea K. Complex regulation of the yeast heat shock transcription factor. Mol Biol Cell. 2000;11:1739-51 pubmed
Kakeya H, Udono H, Maesaki S, Sasaki E, Kawamura S, Hossain M, et al. Heat shock protein 70 (hsp70) as a major target of the antibody response in patients with pulmonary cryptococcosis. Clin Exp Immunol. 1999;115:485-90 pubmed
Botzler C, Li G, Issels R, Multhoff G. Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response. Cell Stress Chaperones. 1998;3:6-11 pubmed
Herbertsson H, Kühme T, Evertsson U, Wigren J, Hammarstrom S. Identification of subunits of the 650 kDa 12(S)-HETE binding complex in carcinoma cells. J Lipid Res. 1998;39:237-44 pubmed
Gabai V, Meriin A, Mosser D, Caron A, Rits S, Shifrin V, et al. Hsp70 prevents activation of stress kinases. A novel pathway of cellular thermotolerance. J Biol Chem. 1997;272:18033-7 pubmed
van den Berg J, Smets L, Van Rooij H. Agonist-free transformation of the glucocorticoid receptor in human B-lymphoma cells. J Steroid Biochem Mol Biol. 1996;57:239-49 pubmed
Richter D, Harn D. Candidate vaccine antigens identified by antibodies from mice vaccinated with 15- or 50-kilorad-irradiated cercariae of Schistosoma mansoni. Infect Immun. 1993;61:146-54 pubmed
Lithgow T, Ryan M, Anderson R, Høj P, Hoogenraad N. A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver. FEBS Lett. 1993;332:277-81 pubmed
Mifflin L, Cohen R. hsc70 moderates the heat shock (stress) response in Xenopus laevis oocytes and binds to denatured protein inducers. J Biol Chem. 1994;269:15718-23 pubmed
Wheeler J, Bieschke E, Tower J. Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress. Proc Natl Acad Sci U S A. 1995;92:10408-12 pubmed
图像
图像 1 :
赛默飞世尔 MA3-007 图像 1
Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50µg of the indicated whole cell lysates and 15?l of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-007) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
图像 2 :
赛默飞世尔 MA3-007 图像 2
Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (5A5) (Product# MA3-007) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-007) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 3 :
赛默飞世尔 MA3-007 图像 3
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human prostate carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Heat Shock Protein 70 (MA3-007) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
HSP70单克隆抗体(5A5)
目录# :
MA3-007
规格 :
250微升
价格 :
美国425.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Amphibian, Avian, Canine, Drosophila, Fish, Flatworm, Human, Mouse, Non-human primate, Rat, Yeast
应用 :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:200, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:250-1:1,000
物种 :
Amphibian, Avian, Canine, Drosophila, Fish, Flatworm, Human, Mouse, Non-human primate, Rat, Yeast
克隆 :
5A5
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
免疫原 :
Human recombinant HSP70 over expressed in E. coli.
格式 :
液体
应用w/稀释 :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:200, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:250-1:1,000
别名 :
68 kDa heat shock protein; 70 kda heat shock protein-2; 70 kilodalton heat shock protein; 87A; 87A locus; 87C locus; Bb; CG18743; CG31359; CG31359-PA; CG31366; CG31366-PA; CG31449; CG6489; chaperone; dHSP70; dhsp70Aa; Dmel CG31359; Dmel CG31366; Dmel_CG31359; Dmel_CG31366; Dm-hsp70; DmHSP70AA; DMHSP7D1; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Hcp70.1; heat shock 10kDa protein 1-like; heat shock 70; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1L; heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70 kDa-like protein 1; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 1B (mapped); heat shock 70kD protein-like 1; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 1-like; heat shock cognate 70-kd protein, tandem duplicate 2; Heat shock protein; heat shock protein 1A; heat shock protein 1B; heat shock protein 1-like; heat shock protein 70; heat shock protein 70.1; heat shock protein 70.2; heat shock protein 70-1; Heat shock protein 70-1l; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein cognate 70, testis; heat shock protein family A (Hsp70) member 1 like; heat shock protein family A (Hsp70) member 1A; heat shock protein family A (Hsp70) member 1B; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; heat shock-related protein hsc70t; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; heat-shock protein 70; Heat-shock-protein-70Aa; Heat-shock-protein-70Bb; HEL S 103; HEL-S-103; hs70; Hsc70t; HSP; Hsp 70; hsp68; Hsp70; Hsp-70; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; hsp70 87C; hsp70 Aa; HSP70 AceyltK77; hsp70(87A); Hsp70(87C); HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; Hsp70.3; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; Hsp70-3; HSP70-87A7; HSP70-87C1; hsp70a; hsp70A1; Hsp70A7d; Hsp70Aa; Hsp-70Aa; Hsp70Aa-PA; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bb-PA; Hsp70Bc; HSP70-HOM; HSP70I; HSP70T; HSP72; Hspa1; Hspa1a; HSPA1B; Hspa1l; Hspa2; Hspa70; HSX70; hum70t; inducible heat shock protein 70; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; Msh5; protein 70; si:ch73-46h18.6; si:dkeyp-69e1.1; Spermatid-specific heat shock protein 70; zgc:174006
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国