产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
HSP70单克隆抗体(2A4)
目录 :
MA3-008
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
2A4
反应物种 :
人类, 小鼠, 大鼠, fruit fly
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 12
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 1:5000; 图 1a
Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
  • 免疫印迹; 大鼠
Neve E, Nordling A, Andersson T, Hellman U, Diczfalusy U, Johansson I, et al. Amidoxime reductase system containing cytochrome b5 type B (CYB5B) and MOSC2 is of importance for lipid synthesis in adipocyte mitochondria. J Biol Chem. 2012;287:6307-17 pubmed 出版商
  • 免疫组化; 人类; 1:500
  • 免疫印迹; 人类; 1:4000
Chehna Patel N, Warty N, Sachdeva G, Khole V. Proteolytic tailoring of the heat shock protein 70 and its implications in the pathogenesis of endometriosis. Fertil Steril. 2011;95:1560-7.e1-3 pubmed 出版商
  • 免疫细胞化学; 人类; 1:3000
  • 免疫印迹; 人类; 1:3000
Brodie K, Henderson B. Differential modulation of BRCA1 and BARD1 nuclear localisation and foci assembly by DNA damage. Cell Signal. 2010;22:291-302 pubmed 出版商
  • 免疫印迹; 人类; 1:5000
Dong Y, Miller L, Sanders J, Somero G. Heat-shock protein 70 (Hsp70) expression in four limpets of the genus Lottia: interspecific variation in constitutive and inducible synthesis correlates with in situ exposure to heat stress. Biol Bull. 2008;215:173-81 pubmed
Fan Y, Guo T, Han X, Liu J, Cai Y, Xue H, et al. Paricalcitol accelerates BACE1 lysosomal degradation and inhibits calpain-1 dependent neuronal loss in APP/PS1 transgenic mice. EBioMedicine. 2019;45:393-407 pubmed 出版商
Wang J, Lei P, Gamil A, Lagos L, Yue Y, Schirmer K, et al. Rainbow Trout (Oncorhynchus Mykiss) Intestinal Epithelial Cells as a Model for Studying Gut Immune Function and Effects of Functional Feed Ingredients. Front Immunol. 2019;10:152 pubmed 出版商
Guerrero C, Bouyssounade D, Zárate S, Isa P, López T, Espinosa R, et al. Heat shock cognate protein 70 is involved in rotavirus cell entry. J Virol. 2002;76:4096-102 pubmed
Liu Y, Mayr S, Opitz Gress A, Zeller C, Lormes W, Baur S, et al. Human skeletal muscle HSP70 response to training in highly trained rowers. J Appl Physiol (1985). 1999;86:101-4 pubmed
Botzler C, Li G, Issels R, Multhoff G. Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response. Cell Stress Chaperones. 1998;3:6-11 pubmed
Carr V, Murphy S, Morimoto R, Farbman A. Small subclass of rat olfactory neurons with specific bulbar projections is reactive with monoclonal antibodies to the HSP70 heat shock protein. J Comp Neurol. 1994;348:150-60 pubmed
Li L, Shen G, Li G. Effects of expressing human Hsp70 and its deletion derivatives on heat killing and on RNA and protein synthesis. Exp Cell Res. 1995;217:460-8 pubmed
图像
图像 1 :
赛默飞世尔 MA3-008 图像 1
Immunofluorescent analysis of Heat Shock Protein 70 (Hsp70) in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 70 (Hsp70) monoclonal antibody (Product # MA3-008) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Heat Shock Protein 70 (Hsp70) staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
图像 2 :
赛默飞世尔 MA3-008 图像 2
Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50ug of the indicated whole cell lysates and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-008) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgM-HRP secondary antibody (Product # 31440) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
图像 3 :
赛默飞世尔 MA3-008 图像 3
Immunohistochemistry was performed on normal biopsies of deparaffinized Human breast tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Heat Shock Protein 70 (MA3-008) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
HSP70单克隆抗体(2A4)
目录# :
MA3-008
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Amphibian, Avian, Drosophila, Fish, Human, Mouse, Rat, Yeast
应用 :
Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 2 µg, Western Blot: 1 µg/mL
物种 :
Amphibian, Avian, Drosophila, Fish, Human, Mouse, Rat, Yeast
克隆 :
2A4
抗体亚型 :
IgM
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
免疫原 :
Human recombinant HSP70 over expressed in E. coli.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 2 µg, Western Blot: 1 µg/mL
别名 :
68 kDa heat shock protein; 70 kda heat shock protein-2; 70 kilodalton heat shock protein; 87A; 87A locus; 87C locus; Bb; CG18743; CG31359; CG31359-PA; CG31366; CG31366-PA; CG31449; CG6489; chaperone; dHSP70; dhsp70Aa; Dmel CG31359; Dmel CG31366; Dmel_CG31359; Dmel_CG31366; Dm-hsp70; DmHSP70AA; DMHSP7D1; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Hcp70.1; heat shock 10kDa protein 1-like; heat shock 70; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1L; heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70 kDa-like protein 1; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 1B (mapped); heat shock 70kD protein-like 1; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 1-like; heat shock cognate 70-kd protein, tandem duplicate 2; Heat shock protein; heat shock protein 1A; heat shock protein 1B; heat shock protein 1-like; heat shock protein 70; heat shock protein 70.1; heat shock protein 70.2; heat shock protein 70-1; Heat shock protein 70-1l; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein cognate 70, testis; heat shock protein family A (Hsp70) member 1 like; heat shock protein family A (Hsp70) member 1A; heat shock protein family A (Hsp70) member 1B; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; heat shock-related protein hsc70t; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; heat-shock protein 70; Heat-shock-protein-70Aa; Heat-shock-protein-70Bb; HEL S 103; HEL-S-103; hs70; Hsc70t; HSP; Hsp 70; hsp68; Hsp70; Hsp-70; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; hsp70 87C; hsp70 Aa; HSP70 AceyltK77; hsp70(87A); Hsp70(87C); HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; Hsp70.3; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; Hsp70-3; HSP70-87A7; HSP70-87C1; hsp70a; hsp70A1; Hsp70A7d; Hsp70Aa; Hsp-70Aa; Hsp70Aa-PA; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bb-PA; Hsp70Bc; HSP70-HOM; HSP70I; HSP70T; HSP72; Hspa1; Hspa1a; HSPA1B; Hspa1l; Hspa2; Hspa70; HSX70; hum70t; inducible heat shock protein 70; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; Msh5; protein 70; si:ch73-46h18.6; si:dkeyp-69e1.1; Spermatid-specific heat shock protein 70; zgc:174006
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国