产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
HSP90甲单克隆抗体(3B6)
目录 :
MA3-010
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3B6
反应物种 :
人类, 小鼠, 大鼠,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 9
参考文献
Yoshikawa N, Yamamoto K, Shimizu N, Yamada S, Morimoto C, Tanaka H. The distinct agonistic properties of the phenylpyrazolosteroid cortivazol reveal interdomain communication within the glucocorticoid receptor. Mol Endocrinol. 2005;19:1110-24 pubmed
Calvert M, Digilio L, Herr J, Coonrod S. Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane. Reprod Biol Endocrinol. 2003;1:27 pubmed
Yoshikawa N, Makino Y, Okamoto K, Morimoto C, Makino I, Tanaka H. Distinct interaction of cortivazol with the ligand binding domain confers glucocorticoid receptor specificity: cortivazol is a specific ligand for the glucocorticoid receptor. J Biol Chem. 2002;277:5529-40 pubmed
Miura T, Ouchida R, Yoshikawa N, Okamoto K, Makino Y, Nakamura T, et al. Functional modulation of the glucocorticoid receptor and suppression of NF-kappaB-dependent transcription by ursodeoxycholic acid. J Biol Chem. 2001;276:47371-8 pubmed
Watanabe A, Miyamoto T, Katoh N, Takahashi Y. Effect of stages of lactation on the concentration of a 90-kilodalton heat shock protein in bovine mammary tissue. J Dairy Sci. 1997;80:2372-9 pubmed
Meng X, Devin J, Sullivan W, Toft D, Baulieu E, Catelli M. Mutational analysis of Hsp90 alpha dimerization and subcellular localization: dimer disruption does not impede "in vivo' interaction with estrogen receptor. J Cell Sci. 1996;109 ( Pt 7):1677-87 pubmed
van den Berg J, Smets L, Van Rooij H. Agonist-free transformation of the glucocorticoid receptor in human B-lymphoma cells. J Steroid Biochem Mol Biol. 1996;57:239-49 pubmed
Nimmesgern E, Hartl F. ATP-dependent protein refolding activity in reticulocyte lysate. Evidence for the participation of different chaperone components. FEBS Lett. 1993;331:25-30 pubmed
van den Berg J, Smets L, Hutchison K, Van Rooij H, van den Elshout M. High levels of non-activated receptors in glucocorticoid-sensitive S49wt mouse lymphoma cells incubated with dexamethasone. J Steroid Biochem Mol Biol. 1994;51:33-40 pubmed
图像
图像 1 :
赛默飞世尔 MA3-010 图像 1
Western blot analysis of Heat Shock Protein 90 (HSP90) was performed by loading 50ug of various whole cell lysates and 15ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HSP90 monoclonal antibody (Product # MA3-010) at a dilution of 1:500 overnight at 4ºC on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
图像 2 :
赛默飞世尔 MA3-010 图像 2
Immunofluorescent analysis of Heat Shock Protein 90 using Heat Shock Protein 90 Monoclonal antibody (3B6) (Product# MA3-010) shows staining in MCF-7 cells. Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 (Product# MA3-010) at a dilution of 1:100-1:200 over night at 4ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
HSP90甲单克隆抗体(3B6)
目录# :
MA3-010
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
牛, 人类, 小鼠, 大鼠
应用 :
Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1:500
物种 :
牛, 人类, 小鼠, 大鼠
克隆 :
3B6
抗体亚型 :
IgG2b
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
免疫原 :
纯化小鼠HSP90
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1:500
别名 :
86kDa; 89kDa; 90-kDa heat shock protein; 90-kDa heat shock protein alpha; akineto; AL024080; AL024147; EL52; epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; fb17b01; heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock 90kDa protein 1, beta; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90; heat shock protein 90 alpha family class A member 1; heat shock protein 90, alpha (cytosolic), class A member 1; heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1; heat shock protein 90-alpha 1; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein alpha; Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-alpha 1; heat shock protein HSP90 alpha; heat shock protein hsp90alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HEL-S-65p; HSP; HSP 86; HSP 90 alpha; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; HSP90; hsp90a; hsp90a protein; hsp90a.1; hsp90aa1; hsp90aa1.1; hsp90alpha; HSP90N; HSPC1; Hspca; HSPCA protein; HSPCAL1; HSPCAL4; HSPN; hypothetical protein; LAP2; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; renal carcinoma antigen NY-REN-38; slo; sloth; TSTA; Tumor-specific transplantation 86 kDa antigen; wu:fb17b01; zgc:86652
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国