产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
HSC70单克隆抗体(13D3)
目录 :
MA3-014
规格 :
50微升
价格 :
美国399.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
13D3
反应物种 :
African green monkey, 仓鼠, 人类, 小鼠, 大鼠, 猫
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-冰冻切片, 抑制或激活实验
更多信息或购买 :
文章摘录数: 24
| 出版应用/物种/样本/稀释 | 参考文献 |
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| David D, Ittner L, Gehrig P, Nergenau D, Shepherd C, Halliday G, et al. Beta-amyloid treatment of two complementary P301L tau-expressing Alzheimer's disease models reveals similar deregulated cellular processes. Proteomics. 2006;6:6566-77 pubmed
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| López T, Lopez S, Arias C. Heat shock enhances the susceptibility of BHK cells to rotavirus infection through the facilitation of entry and post-entry virus replication steps. Virus Res. 2006;121:74-83 pubmed
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Hegmans J, Bard M, Hemmes A, Luider T, Kleijmeer M, Prins J, et al. Proteomic analysis of exosomes secreted by human mesothelioma cells. Am J Pathol. 2004;164:1807-15 pubmed
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Guerrero C, Bouyssounade D, Zárate S, Isa P, López T, Espinosa R, et al. Heat shock cognate protein 70 is involved in rotavirus cell entry. J Virol. 2002;76:4096-102 pubmed
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Thompson H, Clarkson P, Scordilis S. The repeated bout effect and heat shock proteins: intramuscular HSP27 and HSP70 expression following two bouts of eccentric exercise in humans. Acta Physiol Scand. 2002;174:47-56 pubmed
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Fourie A, Peterson P, Yang Y. Characterization and regulation of the major histocompatibility complex-encoded proteins Hsp70-Hom and Hsp70-1/2. Cell Stress Chaperones. 2001;6:282-95 pubmed
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Bernstein S, Liu A, Hansen B, Somiari R. Heat shock cognate-70 gene expression declines during normal aging of the primate retina. Invest Ophthalmol Vis Sci. 2000;41:2857-62 pubmed
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Polanowska Grabowska R, Simon C, Falchetto R, Shabanowitz J, Hunt D, Gear A. Platelet adhesion to collagen under flow causes dissociation of a phosphoprotein complex of heat-shock proteins and protein phosphatase 1. Blood. 1997;90:1516-26 pubmed
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Nakano M, Mann D, Knowlton A. Blocking the endogenous increase in HSP 72 increases susceptibility to hypoxia and reoxygenation in isolated adult feline cardiocytes. Circulation. 1997;95:1523-31 pubmed
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Leung S, Hightower L. A 16-kDa protein functions as a new regulatory protein for Hsc70 molecular chaperone and is identified as a member of the Nm23/nucleoside diphosphate kinase family. J Biol Chem. 1997;272:2607-14 pubmed
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Nunes S, Calderwood S. Heat shock factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cells. Biochem Biophys Res Commun. 1995;213:1-6 pubmed
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Allen R, O Brien D, Eddy E. A novel hsp70-like protein (P70) is present in mouse spermatogenic cells. Mol Cell Biol. 1988;8:828-32 pubmed
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Maekawa M, O Brien D, Allen R, Eddy E. Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells. Biol Reprod. 1989;40:843-52 pubmed
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Terlecky S, Chiang H, Olson T, Dice J. Protein and peptide binding and stimulation of in vitro lysosomal proteolysis by the 73-kDa heat shock cognate protein. J Biol Chem. 1992;267:9202-9 pubmed
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图像
图像 1 :
Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50µg of the indicated whole cell lysates and 15µl of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-014) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM-HRP secondary antibody (Product # 31440) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
图像 2 :
Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (13D3) (Product# MA3-014) shows staining in NIH-3T3 cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-014) at a dilution of 1:20-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
HSC70单克隆抗体(13D3)
目录# :
MA3-014
规格 :
50微升
价格 :
美国399.00
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
Feline, Human, Mouse, Non-human primate, Rat
应用 :
Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:20-1:200, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:4,000
物种 :
Feline, Human, Mouse, Non-human primate, Rat
克隆 :
13D3
抗体亚型 :
IgM
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.
免疫原 :
Mouse spermatogenic cell protein.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:20-1:200, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:4,000
别名 :
2410008N15Rik; AAG2; constitutive heat shock protein 70; constitutively expressed heat shock protein; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate; heat shock cognate 70; heat shock cognate 71 kD protein; heat shock cognate 71 kDa protein; heat shock cognate 71 kDa protein-like protein; heat shock cognate 71-kd protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 70 cognate; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; heat shock protein family A (Hsp70) member 8; HEL-33; HEL-S-72p; HIP; HSC54; Hsc70; Hsc70 ATPase; HSC70 protein; Hsc71; Hsc73; HSP70; HSP71; HSP73; Hspa10; Hspa8; hspa8 protein; I79_022974; LAP; LAP1; LAP-1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; similar to 7O KDa heat shock cognate protein; SNC6; unnamed protein product; wu:fb01g06; wu:fi48b06
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公司信息
赛默飞世尔