产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
HSP27单克隆抗体(G3.1)
目录 :
MA3-015
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
G3.1
反应物种 :
人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
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文章摘录数: 32
出版应用/物种/样本/稀释 | 参考文献 |
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| Carlson R, Vavricka S, Eloranta J, Musch M, Arvans D, Kles K, et al. fMLP induces Hsp27 expression, attenuates NF-kappaB activation, and confers intestinal epithelial cell protection. Am J Physiol Gastrointest Liver Physiol. 2007;292:G1070-8 pubmed
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| Liu Y, Liu H, Han B, Zhang J. Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis. Cancer Res. 2006;66:3248-55 pubmed
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| Sommer S, Cui Y, Brewer G, Fuqua S. The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells. J Steroid Biochem Mol Biol. 2005;97:219-29 pubmed
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| Joo M, Chi J, Lee H. Expressions of HSP70 and HSP27 in hepatocellular carcinoma. J Korean Med Sci. 2005;20:829-34 pubmed
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| Caraglia M, Marra M, Mancinelli F, d Ambrosio G, Massa R, Giordano A, et al. Electromagnetic fields at mobile phone frequency induce apoptosis and inactivation of the multi-chaperone complex in human epidermoid cancer cells. J Cell Physiol. 2005;204:539-48 pubmed
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Nguyen Ho Bouldoires T, Clapéron A, Mergey M, Wendum D, Desbois Mouthon C, Tahraoui S, et al. Mitogen-activated protein kinase-activated protein kinase 2 mediates resistance to hydrogen peroxide-induced oxidative stress in human hepatobiliary cancer cells. Free Radic Biol Med. 2015;89:34-46 pubmed 出版商
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Wen F, Li Y, Tsai H, Lin C, Li C, Liu C, et al. Down-regulation of heat shock protein 27 in neuronal cells and non-neuronal cells expressing mutant ataxin-3. FEBS Lett. 2003;546:307-14 pubmed
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Elpek G, Karaveli S, Simsek T, Keles N, Aksoy N. Expression of heat-shock proteins hsp27, hsp70 and hsp90 in malignant epithelial tumour of the ovaries. APMIS. 2003;111:523-30 pubmed
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Pignatelli D, Ferreira J, Soares P, Costa M, Magalhães M. Immunohistochemical study of heat shock proteins 27, 60 and 70 in the normal human adrenal and in adrenal tumors with suppressed ACTH production. Microsc Res Tech. 2003;61:315-23 pubmed
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Benvenuti S, Cramer R, Quinn C, Bruce J, Zvelebil M, Corless S, et al. Differential proteome analysis of replicative senescence in rat embryo fibroblasts. Mol Cell Proteomics. 2002;1:280-92 pubmed
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Wyttenbach A, Sauvageot O, Carmichael J, Diaz Latoud C, Arrigo A, Rubinsztein D. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum Mol Genet. 2002;11:1137-51 pubmed
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Wyttenbach A, Swartz J, Kita H, Thykjaer T, Carmichael J, Bradley J, et al. Polyglutamine expansions cause decreased CRE-mediated transcription and early gene expression changes prior to cell death in an inducible cell model of Huntington's disease. Hum Mol Genet. 2001;10:1829-45 pubmed
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Huang P, Liu J, Di A, Robinson N, Musch M, Kaetzel M, et al. Regulation of human CLC-3 channels by multifunctional Ca2+/calmodulin-dependent protein kinase. J Biol Chem. 2001;276:20093-100 pubmed
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Gerner C, Frohwein U, Gotzmann J, Bayer E, Gelbmann D, Bursch W, et al. The Fas-induced apoptosis analyzed by high throughput proteome analysis. J Biol Chem. 2000;275:39018-26 pubmed
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Soldes O, Kuick R, Thompson I, Hughes S, Orringer M, Iannettoni M, et al. Differential expression of Hsp27 in normal oesophagus, Barrett's metaplasia and oesophageal adenocarcinomas. Br J Cancer. 1999;79:595-603 pubmed
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Ciocca D, Green S, Elledge R, Clark G, Pugh R, Ravdin P, et al. Heat shock proteins hsp27 and hsp70: lack of correlation with response to tamoxifen and clinical course of disease in estrogen receptor-positive metastatic breast cancer (a Southwest Oncology Group Study). Clin Cancer Res. 1998;4:1263-6 pubmed
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Ito T, Kawabe R, Kurasono Y, Hara M, Kitamura H, Fujita K, et al. Expression of heat shock proteins in squamous cell carcinoma of the tongue: an immunohistochemical study. J Oral Pathol Med. 1998;27:18-22 pubmed
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Adams D, Hajj H, Edwards D, Bjercke R, McGuire W. Detection of a Mr 24,000 estrogen-regulated protein in human breast cancer by monoclonal antibodies. Cancer Res. 1983;43:4297-301 pubmed
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Ciocca D, Asch R, Adams D, McGuire W. Evidence for modulation of a 24K protein in human endometrium during the menstrual cycle. J Clin Endocrinol Metab. 1983;57:496-9 pubmed
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Adams D, McGuire W. Quantitative enzyme-linked immunosorbent assay for the estrogen-regulated Mr 24,000 protein in human breast tumors: correlation with estrogen and progesterone receptors. Cancer Res. 1985;45:2445-9 pubmed
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Dressler L, Ramzy I, Sledge G, McGuire W. A new marker of maturation in the cervix: the estrogen-regulated 24K protein. Obstet Gynecol. 1986;68:825-31 pubmed
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Moretti Rojas I, Fuqua S, Montgomery R, McGuire W. A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Breast Cancer Res Treat. 1988;11:155-63 pubmed
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McGuire S, Fuqua S, Naylor S, Helin Davis D, McGuire W. Chromosomal assignments of human 27-kDa heat shock protein gene family. Somat Cell Mol Genet. 1989;15:167-71 pubmed
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图像
图像 1 :
Western blot analysis of Heat Shock Protein 27 (HSP27) was performed by loading 50µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP27 monoclonal antibody (Product # MA3-015) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
产品信息
产品类型 :
抗体
产品名称 :
HSP27单克隆抗体(G3.1)
目录# :
MA3-015
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
Canine, Human, Non-human primate, Rat
应用 :
ELISA: Assay-dependent, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:500, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µL, Western Blot: 1:500-1:1,000
物种 :
Canine, Human, Non-human primate, Rat
克隆 :
G3.1
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
免疫原 :
Partially purified human HSP27.
格式 :
液体
应用w/稀释 :
ELISA: Assay-dependent, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:500, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µL, Western Blot: 1:500-1:1,000
别名 :
27kDa; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 1; heat shock protein 27 kDa beta-1; heat shock protein B1; heat shock protein beta-1; heat shock protein family B (small) member 1; heat shock protein, 25 kDa; heat-shock protein; HEL-S-102; HMN2B; HS; HS.76067; HSP; HSP 25; HSP 27; Hsp25; HSP27; HSP28; HSPB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25
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公司信息
赛默飞世尔