产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
PDI单克隆抗体(RL90)
目录 :
MA3-019
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
RL90
反应物种 :
中国人仓鼠, African green monkey, 人类, 小鼠, 大鼠, pigs , 猕猴
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片, 抑制或激活实验
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 80
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 图 7c
Haag A, Walser M, Henggeler A, Hajnal A. The CHORD protein CHP-1 regulates EGF receptor trafficking and signaling in C. elegans and in human cells. elife. 2020;9: pubmed 出版商
  • 免疫细胞化学; 小鼠; 图 6a
Fukushima M, Dasgupta D, Mauer A, Kakazu E, Nakao K, Malhi H. StAR-related lipid transfer domain 11 (STARD11)-mediated ceramide transport mediates extracellular vesicle biogenesis. J Biol Chem. 2018;293:15277-15289 pubmed 出版商
  • 免疫印迹; 小鼠; 图 6a
Moretti A, Pavanelli J, Nolasco P, Leisegang M, Tanaka L, Fernandes C, et al. Conserved Gene Microsynteny Unveils Functional Interaction Between Protein Disulfide Isomerase and Rho Guanine-Dissociation Inhibitor Families. Sci Rep. 2017;7:17262 pubmed 出版商
  • 免疫细胞化学; 人类; 1:300; 图 6b
Waters A, Ozkan Dagliyan I, Vaseva A, Fer N, Strathern L, Hobbs G, et al. Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. Sci Signal. 2017;10: pubmed 出版商
  • 免疫细胞化学; 人类; 1:500; 图 4a
Yamashita Y, Anczurowski M, Nakatsugawa M, Tanaka M, Kagoya Y, Sinha A, et al. HLA-DP84Gly constitutively presents endogenous peptides generated by the class I antigen processing pathway. Nat Commun. 2017;8:15244 pubmed 出版商
  • 免疫印迹; 大鼠; 图 2c
Wang H, Tri Anggraini F, Chen X, DeGracia D. Embryonic lethal abnormal vision proteins and adenine and uridine-rich element mRNAs after global cerebral ischemia and reperfusion in the rat. J Cereb Blood Flow Metab. 2017;37:1494-1507 pubmed 出版商
  • 免疫细胞化学; 人类; 50 ug/ml; 图 4
Pacello F, D Orazio M, Battistoni A. An ERp57-mediated disulphide exchange promotes the interaction between Burkholderia cenocepacia and epithelial respiratory cells. Sci Rep. 2016;6:21140 pubmed 出版商
  • 免疫细胞化学; 人类; 图 s9
Rost B, Schneider F, Grauel M, Wozny C, Bentz C, Blessing A, et al. Optogenetic acidification of synaptic vesicles and lysosomes. Nat Neurosci. 2015;18:1845-1852 pubmed 出版商
  • 免疫细胞化学; 大鼠
Cheng D, Weckerle A, Yu Y, Ma L, Zhu X, Murea M, et al. Biogenesis and cytotoxicity of APOL1 renal risk variant proteins in hepatocytes and hepatoma cells. J Lipid Res. 2015;56:1583-93 pubmed 出版商
  • 免疫印迹; 人类; 1:2000; 图 1b
Janes K. An analysis of critical factors for quantitative immunoblotting. Sci Signal. 2015;8:rs2 pubmed 出版商
  • 免疫细胞化学; 猕猴; 图 2
van Bergeijk P, Adrian M, Hoogenraad C, Kapitein L. Optogenetic control of organelle transport and positioning. Nature. 2015;518:111-114 pubmed 出版商
  • 免疫印迹; 人类; 图 1,2,3,4,5,6
Cherepanova N, Shrimal S, Gilmore R. Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins. J Cell Biol. 2014;206:525-39 pubmed 出版商
  • 免疫印迹; 人类
Saito R, Kaneko M, Kitamura Y, Takata K, Kawada K, Okuma Y, et al. Effects of oxidative stress on the solubility of HRD1, a ubiquitin ligase implicated in Alzheimer's disease. PLoS ONE. 2014;9:e94576 pubmed 出版商
  • 免疫印迹; 大鼠; 1:20000, 1:100000
Wlcek K, Hofstetter L, Stieger B. Transport of estradiol-17?-glucuronide, estrone-3-sulfate and taurocholate across the endoplasmic reticulum membrane: evidence for different transport systems. Biochem Pharmacol. 2014;88:106-18 pubmed 出版商
  • 免疫细胞化学; 人类
Matsumoto M, Katsuyama M, Iwata K, Ibi M, Zhang J, Zhu K, et al. Characterization of N-glycosylation sites on the extracellular domain of NOX1/NADPH oxidase. Free Radic Biol Med. 2014;68:196-204 pubmed 出版商
  • 免疫组化-冰冻切片; 大鼠; 1:200
  • 免疫组化; 大鼠; 1:200
Lewis M, Jamison J, Dunbar J, DeGracia D. mRNA redistribution during permanent focal cerebral ischemia. Transl Stroke Res. 2013;4:604-17 pubmed 出版商
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Numata Y, Morimura T, Nakamura S, Hirano E, Kure S, Goto Y, et al. Depletion of molecular chaperones from the endoplasmic reticulum and fragmentation of the Golgi apparatus associated with pathogenesis in Pelizaeus-Merzbacher disease. J Biol Chem. 2013;288:7451-66 pubmed 出版商
  • 流式细胞仪; 猕猴
Calderon M, Guerrero C, Acosta O, Lopez S, Arias C. Inhibiting rotavirus infection by membrane-impermeant thiol/disulfide exchange blockers and antibodies against protein disulfide isomerase. Intervirology. 2012;55:451-64 pubmed 出版商
  • 免疫印迹; 人类
Buschow S, Lasonder E, Szklarczyk R, Oud M, de Vries I, Figdor C. Unraveling the human dendritic cell phagosome proteome by organellar enrichment ranking. J Proteomics. 2012;75:1547-62 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100
Messaed C, Akoury E, Djuric U, Zeng J, Saleh M, Gilbert L, et al. NLRP7, a nucleotide oligomerization domain-like receptor protein, is required for normal cytokine secretion and co-localizes with Golgi and the microtubule-organizing center. J Biol Chem. 2011;286:43313-23 pubmed 出版商
  • 免疫组化-冰冻切片; 大鼠; 1:200
  • 免疫组化; 大鼠; 1:200
  • 免疫印迹; 大鼠; 1:1000
Jamison J, Szymanski J, DeGracia D. Organelles do not colocalize with mRNA granules in post-ischemic neurons. Neuroscience. 2011;199:394-400 pubmed 出版商
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Matsushita E, Asai N, Enomoto A, Kawamoto Y, Kato T, Mii S, et al. Protective role of Gipie, a Girdin family protein, in endoplasmic reticulum stress responses in endothelial cells. Mol Biol Cell. 2011;22:736-47 pubmed 出版商
  • 免疫细胞化学; 人类; 1:1000
Wen X, Abe T, Kukihara H, Taguwa S, Mori Y, Tani H, et al. Elimination of hepatitis C virus from hepatocytes by a selective activation of therapeutic molecules. PLoS ONE. 2011;6:e15967 pubmed 出版商
  • 免疫细胞化学; 大鼠
Nevalainen M, Kaisto T, Metsikkö K. Mobile ER-to-Golgi but not post-Golgi membrane transport carriers disappear during the terminal myogenic differentiation. Cell Tissue Res. 2010;342:107-16 pubmed 出版商
  • 免疫细胞化学; African green monkey; 1:100
Yuasa N, Zhang W, Goto T, Sakaue H, Matsumoto Takasaki A, Kimura M, et al. Production of anti-carbohydrate antibodies by phage display technologies: potential impairment of cell growth as a result of endogenous expression. J Biol Chem. 2010;285:30587-97 pubmed 出版商
  • 免疫细胞化学; 小鼠
Carnegie J, Robert Cooperman C, Wu J, Young R, Wolf B, Burkhardt B. Characterization of the expression, localization, and secretion of PANDER in alpha-cells. Mol Cell Endocrinol. 2010;325:36-45 pubmed 出版商
  • 免疫沉淀; 人类; 10 ug/ml
  • 免疫印迹; 人类; 1 ug/ml
Swiatkowska M, Padula G, Michalec L, Stasiak M, Skurzynski S, Cierniewski C. Ero1alpha is expressed on blood platelets in association with protein-disulfide isomerase and contributes to redox-controlled remodeling of alphaIIbbeta3. J Biol Chem. 2010;285:29874-83 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100
Eleveld Trancikova D, Sanecka A, van Hout Kuijer M, Looman M, Hendriks I, Jansen B, et al. DC-STAMP interacts with ER-resident transcription factor LUMAN which becomes activated during DC maturation. Mol Immunol. 2010;47:1963-73 pubmed 出版商
  • 免疫细胞化学; 人类; 1:1000
Schmidt J, Yvone G, Brown W. Membrane topology of human AGPAT3 (LPAAT3). Biochem Biophys Res Commun. 2010;397:661-7 pubmed 出版商
  • 免疫印迹; 人类
Unsworth H, Raguz S, Edwards H, Higgins C, Yague E. mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum. FASEB J. 2010;24:3370-80 pubmed 出版商
  • 免疫印迹; 人类
Kaneko M, Koike H, Saito R, Kitamura Y, Okuma Y, Nomura Y. Loss of HRD1-mediated protein degradation causes amyloid precursor protein accumulation and amyloid-beta generation. J Neurosci. 2010;30:3924-32 pubmed 出版商
  • 免疫组化; 人类
Hino S, Kondo S, Yoshinaga K, Saito A, Murakami T, Kanemoto S, et al. Regulation of ER molecular chaperone prevents bone loss in a murine model for osteoporosis. J Bone Miner Metab. 2010;28:131-8 pubmed 出版商
  • 免疫沉淀; 小鼠
Santos C, Stolf B, Takemoto P, Amanso A, Lopes L, Souza E, et al. Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages. J Leukoc Biol. 2009;86:989-98 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100
Pyle A, Li B, Maupin A, Guzman R, Crimmins D, Olson S, et al. Biomechanical stress induces novel arterial intima-enriched genes: implications for vascular adaptation to stress. Cardiovasc Pathol. 2010;19:e13-20 pubmed 出版商
  • 免疫细胞化学; 人类
Ludwig A, Blume J, Diep T, Yuan J, Mateos J, Leuthäuser K, et al. Calsyntenins mediate TGN exit of APP in a kinesin-1-dependent manner. Traffic. 2009;10:572-89 pubmed 出版商
  • 流式细胞仪; 人类
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Swiatkowska M, Szymanski J, Padula G, Cierniewski C. Interaction and functional association of protein disulfide isomerase with alphaVbeta3 integrin on endothelial cells. FEBS J. 2008;275:1813-23 pubmed 出版商
  • 免疫印迹; 小鼠
Gramolini A, Kislinger T, Alikhani Koopaei R, Fong V, Thompson N, Isserlin R, et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008;7:519-33 pubmed
  • 抑制或激活实验; 人类; 1:500
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Manickam N, Sun X, Li M, Gazitt Y, Essex D. Protein disulphide isomerase in platelet function. Br J Haematol. 2008;140:223-9 pubmed
  • 免疫沉淀; 小鼠
  • 免疫沉淀; 人类
Mohler P, Zhu M, Blade A, Ham A, Shelness G, Swift L. Identification of a novel isoform of microsomal triglyceride transfer protein. J Biol Chem. 2007;282:26981-8 pubmed
  • 免疫组化; 人类; 1:300
Csaba Z, Lelouvier B, Viollet C, El Ghouzzi V, Toyama K, Videau C, et al. Activated somatostatin type 2 receptors traffic in vivo in central neurons from dendrites to the trans Golgi before recycling. Traffic. 2007;8:820-34 pubmed
  • 免疫组化; 大鼠
Nissinen M, Kaisto T, Salmela P, Peltonen J, Metsikkö K. Restricted distribution of mRNAs encoding a sarcoplasmic reticulum or transverse tubule protein in skeletal myofibers. J Histochem Cytochem. 2005;53:217-27 pubmed
  • 免疫细胞化学; 人类
Eleveld Trancikova D, Triantis V, Moulin V, Looman M, Wijers M, Fransen J, et al. The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum. J Leukoc Biol. 2005;77:337-43 pubmed
Chen M, Amado N, Tan J, Reis A, Ge M, Abreu J, et al. TMEM79/MATTRIN defines a pathway for Frizzled regulation and is required for Xenopus embryogenesis. elife. 2020;9: pubmed 出版商
Lin H, Lo Y, Wang W, Huang K, I K, Chang G. Overexpression of FAM46A, a Non-canonical Poly(A) Polymerase, Promotes Hemin-Induced Hemoglobinization in K562 Cells. Front Cell Dev Biol. 2020;8:414 pubmed 出版商
Ma Lauer Y, Zheng Y, Malesevic M, von Brunn B, Fischer G, von Brunn A. Influences of cyclosporin A and non-immunosuppressive derivatives on cellular cyclophilins and viral nucleocapsid protein during human coronavirus 229E replication. Antiviral Res. 2020;173:104620 pubmed 出版商
Alenquer M, Vale Costa S, Etibor T, Ferreira F, Sousa A, Amorim M. Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites. Nat Commun. 2019;10:1629 pubmed 出版商
Komura H, Kakio S, Sasahara T, Arai Y, Takino N, Sato M, et al. Alzheimer Aβ Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKα3 Neurons by Secretion. iScience. 2019;: pubmed 出版商
De Bessa T, Pagano A, Moretti A, Oliveira P, Mendonça S, Kovacic H, et al. Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas. Cell Death Dis. 2019;10:143 pubmed 出版商
Gao Q, Yang Z, Xu S, Li X, Yang X, Jin P, et al. Heterotypic CAF-tumor spheroids promote early peritoneal metastatis of ovarian cancer. J Exp Med. 2019;216:688-703 pubmed 出版商
Araujo T, Venturini G, Moretti A, Tanaka L, Pereira A, Laurindo F. Cell-surface HSP70 associates with thrombomodulin in endothelial cells. Cell Stress Chaperones. 2019;24:273-282 pubmed 出版商
Bravo Sagua R, Parra V, Ortiz Sandoval C, Navarro Marquez M, Rodriguez A, Diaz Valdivia N, et al. Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER-mitochondria communication during the early phase of ER stress. Cell Death Differ. 2019;26:1195-1212 pubmed 出版商
Lambert L, Dubayle D, Fafouri A, Herzog E, Csaba Z, Dournaud P, et al. Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway. Front Cell Neurosci. 2018;12:450 pubmed 出版商
Lobet E, Willemart K, Ninane N, Demazy C, Sedzicki J, Lelubre C, et al. Mitochondrial fragmentation affects neither the sensitivity to TNFα-induced apoptosis of Brucella-infected cells nor the intracellular replication of the bacteria. Sci Rep. 2018;8:5173 pubmed 出版商
Weidner J, Jarenbäck L, Åberg I, Westergren Thorsson G, Ankerst J, Bjermer L, et al. Endoplasmic reticulum, Golgi, and lysosomes are disorganized in lung fibroblasts from chronic obstructive pulmonary disease patients. Physiol Rep. 2018;6: pubmed 出版商
Yi M, Melkonian A, Ousey J, Khosla C. Endoplasmic reticulum-resident protein 57 (ERp57) oxidatively inactivates human transglutaminase 2. J Biol Chem. 2018;293:2640-2649 pubmed 出版商
Yen J, Wei L, Chen L, Chen L, Hung C, Wang S, et al. Subcellular Localization and Functional Characterization of GII.4 Norovirus-Encoded NTPase. J Virol. 2018;92: pubmed 出版商
Janickova H, Prado V, Prado M, El Mestikawy S, Bernard V. Vesicular acetylcholine transporter (VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function. J Neurochem. 2017;142:857-875 pubmed 出版商
Manuel A, Walla M, Faccenda A, Martin S, Tanis R, Piroli G, et al. Succination of Protein Disulfide Isomerase Links Mitochondrial Stress and Endoplasmic Reticulum Stress in the Adipocyte During Diabetes. Antioxid Redox Signal. 2017;27:1281-1296 pubmed 出版商
Tanaka L, Araújo H, Hironaka G, Araujo T, Takimura C, Rodriguez A, et al. Peri/Epicellular Protein Disulfide Isomerase Sustains Vascular Lumen Caliber Through an Anticonstrictive Remodeling Effect. Hypertension. 2016;67:613-22 pubmed 出版商
Yamamoto T, Shimojima K, Sangu N, Komoike Y, Ishii A, Abe S, et al. Single nucleotide variations in CLCN6 identified in patients with benign partial epilepsies in infancy and/or febrile seizures. PLoS ONE. 2015;10:e0118946 pubmed 出版商
Suzuki M, Otsuka T, Ohsaki Y, Cheng J, Taniguchi T, Hashimoto H, et al. Derlin-1 and UBXD8 are engaged in dislocation and degradation of lipidated ApoB-100 at lipid droplets. Mol Biol Cell. 2012;23:800-10 pubmed 出版商
Wu Y, Ahmad S, Zhou J, Wang L, Cully M, Essex D. The disulfide isomerase ERp57 mediates platelet aggregation, hemostasis, and thrombosis. Blood. 2012;119:1737-46 pubmed 出版商
Lelouvier B, Tamagno G, Kaindl A, Roland A, Lelievre V, Le Verche V, et al. Dynamics of somatostatin type 2A receptor cargoes in living hippocampal neurons. J Neurosci. 2008;28:4336-49 pubmed 出版商
Yamaguchi Y, Larkin D, Lara Lemus R, Ramos Castaneda J, Liu M, Arvan P. Endoplasmic reticulum (ER) chaperone regulation and survival of cells compensating for deficiency in the ER stress response kinase, PERK. J Biol Chem. 2008;283:17020-9 pubmed 出版商
Blagoveshchenskaya A, Cheong F, Rohde H, Glover G, Knödler A, Nicolson T, et al. Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1. J Cell Biol. 2008;180:803-12 pubmed 出版商
Janiszewski M, Lopes L, Carmo A, Pedro M, Brandes R, Santos C, et al. Regulation of NAD(P)H oxidase by associated protein disulfide isomerase in vascular smooth muscle cells. J Biol Chem. 2005;280:40813-9 pubmed
Jordan P, Stevens J, Hubbard G, Barrett N, Sage T, Authi K, et al. A role for the thiol isomerase protein ERP5 in platelet function. Blood. 2005;105:1500-7 pubmed
Mizrachi D, Segaloff D. Intracellularly located misfolded glycoprotein hormone receptors associate with different chaperone proteins than their cognate wild-type receptors. Mol Endocrinol. 2004;18:1768-77 pubmed
Flores Díaz M, Higuita J, Florin I, Okada T, Pollesello P, Bergman T, et al. A cellular UDP-glucose deficiency causes overexpression of glucose/oxygen-regulated proteins independent of the endoplasmic reticulum stress elements. J Biol Chem. 2004;279:21724-31 pubmed
Markovic I, Stantchev T, Fields K, Tiffany L, Tomic M, Weiss C, et al. Thiol/disulfide exchange is a prerequisite for CXCR4-tropic HIV-1 envelope-mediated T-cell fusion during viral entry. Blood. 2004;103:1586-94 pubmed
Lahav J, Wijnen E, Hess O, Hamaia S, Griffiths D, Makris M, et al. Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin alpha2beta1. Blood. 2003;102:2085-92 pubmed
Davis C, Raulston J, Wyrick P. Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells. Infect Immun. 2002;70:3413-8 pubmed
Nies A, Konig J, Cui Y, Brom M, Spring H, Keppler D. Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2). Eur J Biochem. 2002;269:1866-76 pubmed
Vranka J, Mokashi A, Keene D, Tufa S, Corson G, Sussman M, et al. Selective intracellular retention of extracellular matrix proteins and chaperones associated with pseudoachondroplasia. Matrix Biol. 2001;20:439-50 pubmed
Barkalow F, Barkalow K, Mayadas T. Dimerization of P-selectin in platelets and endothelial cells. Blood. 2000;96:3070-7 pubmed
Bennett T, Edwards B, Sklar L, Rogelj S. Sulfhydryl regulation of L-selectin shedding: phenylarsine oxide promotes activation-independent L-selectin shedding from leukocytes. J Immunol. 2000;164:4120-9 pubmed
Tanaka S, Uehara T, Nomura Y. Up-regulation of protein-disulfide isomerase in response to hypoxia/brain ischemia and its protective effect against apoptotic cell death. J Biol Chem. 2000;275:10388-93 pubmed
Mezghrani A, Courageot J, Mani J, Pugniere M, Bastiani P, Miquelis R. Protein-disulfide isomerase (PDI) in FRTL5 cells. pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes. J Biol Chem. 2000;275:1920-9 pubmed
Ryser H, Levy E, Mandel R, DiSciullo G. Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction. Proc Natl Acad Sci U S A. 1994;91:4559-63 pubmed
Kaetzel C, Rao C, Lamm M. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J. 1987;241:39-47 pubmed
图像
图像 1 :
赛默飞世尔 MA3-019 图像 1
Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
产品信息
产品类型 :
抗体
产品名称 :
PDI单克隆抗体(RL90)
目录# :
MA3-019
规格 :
100微升
价格 :
美国425.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
仓鼠, 人类, 小鼠, 猪, 大鼠
应用 :
Flow Cytometry: 0.5-1 µg/test, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1:100, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:200-1:2,000
物种 :
仓鼠, 人类, 小鼠, 猪, 大鼠
克隆 :
RL90
抗体亚型 :
IgG2a
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
免疫原 :
纯化大鼠PDI蛋白
格式 :
液体
应用w/稀释 :
Flow Cytometry: 0.5-1 µg/test, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1:100, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:200-1:2,000
别名 :
1810041F13Rik; AI661267; CaO19.12595; cellular thyroid hormone binding protein; cellular thyroid hormone-binding protein; CLCRP1; collagen prolyl 4-hydroxylase beta; DSI; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; ERBA2L; ERp59; GIT; glutathione-insulin transhydrogenase; I79_002399; LOW QUALITY PROTEIN: protein disulfide-isomerase A2; MFP1; P4HB; p4hb.L; P4Hbeta; p55; p58; Pancreas-specific protein disulfide isomerase; pancreatic protein disulfide isomerase; PDA2; PDI; PDI (E.C.5.3.4.1); PDI1; PDI1P; Pdia1; pdia2; pdia2.L; PDIP; Pdipl; PDIR; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; PROHB; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase subunit beta L homeolog; prolyl 4-hydroxylase subunit beta L homeolog; prolyl 4-hydroxylase, beta polypeptide L homeolog; prolyl 4-hydroxylase, beta polypeptide; prolyl 4-hydroxylase, beta subunit; protein disulfide isomerase; protein disulfide isomerase (pancreas) like; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase A2; protein disulfide isomerase associated 2; protein disulfide isomerase family A member 2; protein disulfide isomerase family A member 2 L homeolog; protein disulfide isomerase family A, member 1; protein disulfide isomerase family A, member 2; protein disulfide isomerase PDI1; protein disulfide isomerase, pancreatic; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide isomerase-associated 2; protein disulfide-isomerase; Protein disulfide-isomerase A2; protocollagen hydroxylase; Rho GDP dissociation inhibitor gamma; testicular secretory protein Li 32; Thbp; Thioredoxin-related glycoprotein 1; thyroid hormone-binding protein p55; TRG1; XELAEV_18043753mg; XELAEV_18045582mg; XPDIp; YCL043C; YCL313; YCL43C
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