产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
钙联接蛋白单克隆抗体(AF18)
目录 :
MA3-027
规格 :
100微升
价格 :
美国399.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AF18
反应物种 :
African green monkey, 人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-冰冻切片, 核糖核酸免疫沉淀
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文章摘录数: 48
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| Medigeshi G, Lancaster A, Hirsch A, Briese T, Lipkin W, DeFilippis V, et al. West Nile virus infection activates the unfolded protein response, leading to CHOP induction and apoptosis. J Virol. 2007;81:10849-60 pubmed
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| Marrocco D, Tilley W, Bianco Miotto T, Evdokiou A, Scher H, Rifkind R, et al. Suberoylanilide hydroxamic acid (vorinostat) represses androgen receptor expression and acts synergistically with an androgen receptor antagonist to inhibit prostate cancer cell proliferation. Mol Cancer Ther. 2007;6:51-60 pubmed
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| Zhang H, Schmidt B, Sun F, Condliffe S, Butterworth M, Youker R, et al. Cysteine string protein monitors late steps in cystic fibrosis transmembrane conductance regulator biogenesis. J Biol Chem. 2006;281:11312-21 pubmed
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| Kerov V, Natochin M, Artemyev N. Interaction of transducin-alpha with LGN, a G-protein modulator expressed in photoreceptor cells. Mol Cell Neurosci. 2005;28:485-95 pubmed
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| Barre L, Magdalou J, Netter P, Fournel Gigleux S, Ouzzine M. The stop transfer sequence of the human UDP-glucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms. FEBS J. 2005;272:1063-71 pubmed
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Chin K, Zhou H, Wong C, Lee J, Chan C, Qiang B, et al. The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma. Nucleic Acids Res. 2005;33:1859-73 pubmed
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Khanna R, Lee E, Papazian D. Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER. J Cell Sci. 2004;117:2897-908 pubmed
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Krumerman A, Gao X, Bian J, Melman Y, Kagan A, McDonald T. An LQT mutant minK alters KvLQT1 trafficking. Am J Physiol Cell Physiol. 2004;286:C1453-63 pubmed
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Enninga J, Levay A, Fontoura B. Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex. Mol Cell Biol. 2003;23:7271-84 pubmed
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Calvert M, Digilio L, Herr J, Coonrod S. Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane. Reprod Biol Endocrinol. 2003;1:27 pubmed
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Offterdinger M, Schöfer C, Weipoltshammer K, Grunt T. c-erbB-3: a nuclear protein in mammary epithelial cells. J Cell Biol. 2002;157:929-39 pubmed
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Zhang H, Peters K, Sun F, Marino C, Lang J, Burgoyne R, et al. Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator. J Biol Chem. 2002;277:28948-58 pubmed
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Desai B, Myers B, Schreiber S. FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction. Proc Natl Acad Sci U S A. 2002;99:4319-24 pubmed
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Li P, Nakanishi A, Clark S, Kasamatsu H. Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm. Proc Natl Acad Sci U S A. 2002;99:1353-8 pubmed
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Ridsdale R, Tseu I, Wang J, Post M. CTP:phosphocholine cytidylyltransferase alpha is a cytosolic protein in pulmonary epithelial cells and tissues. J Biol Chem. 2001;276:49148-55 pubmed
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Khanna R, Myers M, Laine M, Papazian D. Glycosylation increases potassium channel stability and surface expression in mammalian cells. J Biol Chem. 2001;276:34028-34 pubmed
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Walenta J, Didier A, Liu X, Kramer H. The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. J Cell Biol. 2001;152:923-34 pubmed
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Khoo K, Han M, Park J, Chae S, Kim U, Lee H, et al. Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus. J Biol Chem. 2000;275:24807-17 pubmed
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Meacham G, Lu Z, King S, Sorscher E, Tousson A, Cyr D. The Hdj-2/Hsc70 chaperone pair facilitates early steps in CFTR biogenesis. EMBO J. 1999;18:1492-505 pubmed
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Lindquist J, Jensen O, Mann M, Hammerling G. ER-60, a chaperone with thiol-dependent reductase activity involved in MHC class I assembly. EMBO J. 1998;17:2186-95 pubmed
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Rajagopalan S, Xu Y, Brenner M. Retention of unassembled components of integral membrane proteins by calnexin. Science. 1994;263:387-90 pubmed
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Galvin K, Krishna S, Ponchel F, Frohlich M, Cummings D, Carlson R, et al. The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene. Proc Natl Acad Sci U S A. 1992;89:8452-6 pubmed
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图像
图像 1 :
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human hepatocarcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Anti-Calnexin (MA3-027) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
钙联接蛋白单克隆抗体(AF18)
目录# :
MA3-027
规格 :
100微升
价格 :
美国399.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
人类, 小鼠
应用 :
Immunocytochemistry: 1:50-1:500, Immunohistochemistry: 1:20, Immunoprecipitation: 5 µg, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000
物种 :
人类, 小鼠
克隆 :
AF18
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).
免疫原 :
Focus human hepatoma cell lysate.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:50-1:500, Immunohistochemistry: 1:20, Immunoprecipitation: 5 µg, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000
别名 :
1110069N15Rik; AI988026; calmegin; calnexin; canx; canx-a; canx-b; clgn; clnx; cnx; D11Ertd153e; FLJ26570; hypothetical protein; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; P90; PP90; pp90 precursor
更多信息或购买 :
公司信息
赛默飞世尔