产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
TGN46单克隆抗体(2F7.1)
目录 :
MA3-063
规格 :
100微克
价格 :
美国399.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
2F7.1
反应物种 :
African green monkey, 人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 49
出版应用/物种/样本/稀释参考文献
  • 免疫组化-冰冻切片; 大鼠; 1:200
  • 免疫组化; 大鼠; 1:200
Lewis M, Jamison J, Dunbar J, DeGracia D. mRNA redistribution during permanent focal cerebral ischemia. Transl Stroke Res. 2013;4:604-17 pubmed 出版商
  • 免疫组化-冰冻切片; 大鼠; 1:200
  • 免疫组化; 大鼠; 1:200
  • 免疫印迹; 大鼠; 1:250
Jamison J, Szymanski J, DeGracia D. Organelles do not colocalize with mRNA granules in post-ischemic neurons. Neuroscience. 2011;199:394-400 pubmed 出版商
  • 免疫细胞化学; 人类
Cammisotto P, Bendayan M, Sane A, Dominguez M, Garofalo C, Levy E. Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro. Int J Cell Biol. 2010;2010:928169 pubmed 出版商
  • 免疫细胞化学; African green monkey; 图 1
Fukura N, Ohgaki R, Matsushita M, Nakamura N, Mitsui K, Kanazawa H. A membrane-proximal region in the C-terminal tail of NHE7 is required for its distribution in the trans-Golgi network, distinct from NHE6 localization at endosomes. J Membr Biol. 2010;234:149-58 pubmed 出版商
  • 免疫组化; 人类; 1:500
Csaba Z, Lelouvier B, Viollet C, El Ghouzzi V, Toyama K, Videau C, et al. Activated somatostatin type 2 receptors traffic in vivo in central neurons from dendrites to the trans Golgi before recycling. Traffic. 2007;8:820-34 pubmed
  • 免疫细胞化学; 小鼠
Follit J, Tuft R, Fogarty K, Pazour G. The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly. Mol Biol Cell. 2006;17:3781-92 pubmed
  • 免疫细胞化学; 大鼠
Young J, Stauber T, del Nery E, Vernos I, Pepperkok R, Nilsson T. Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'. Mol Biol Cell. 2005;16:162-77 pubmed
Gorinski N, Bijata M, Prasad S, Wirth A, Abdel Galil D, Zeug A, et al. Attenuated palmitoylation of serotonin receptor 5-HT1A affects receptor function and contributes to depression-like behaviors. Nat Commun. 2019;10:3924 pubmed 出版商
Vest K, Paskavitz A, Lee J, Padilla Benavides T. Dynamic changes in copper homeostasis and post-transcriptional regulation of Atp7a during myogenic differentiation. Metallomics. 2018;10:309-322 pubmed 出版商
Jean Louis T, Rockwell P, Figueiredo Pereira M. Prostaglandin J2 promotes O-GlcNAcylation raising APP processing by α- and β-secretases: relevance to Alzheimer's disease. Neurobiol Aging. 2018;62:130-145 pubmed 出版商
Rhett J, Calder B, Fann S, Bainbridge H, Gourdie R, Yost M. Mechanism of action of the anti-inflammatory connexin43 mimetic peptide JM2. Am J Physiol Cell Physiol. 2017;313:C314-C326 pubmed 出版商
Janickova H, Prado V, Prado M, El Mestikawy S, Bernard V. Vesicular acetylcholine transporter (VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function. J Neurochem. 2017;142:857-875 pubmed 出版商
Blanco E, Lagos C, Andrés M, Gysling K. An amphipathic alpha-helix in the prodomain of cocaine and amphetamine regulated transcript peptide precursor serves as its sorting signal to the regulated secretory pathway. PLoS ONE. 2013;8:e59695 pubmed 出版商
Lelouvier B, Tamagno G, Kaindl A, Roland A, Lelievre V, Le Verche V, et al. Dynamics of somatostatin type 2A receptor cargoes in living hippocampal neurons. J Neurosci. 2008;28:4336-49 pubmed 出版商
Mitra S, Annamalai L, Chakraborty S, Johnson K, Song X, Batra S, et al. Androgen-regulated formation and degradation of gap junctions in androgen-responsive human prostate cancer cells. Mol Biol Cell. 2006;17:5400-16 pubmed
Sánchez Ruiloba L, Cabrera Poch N, Rodríguez Martínez M, López Menéndez C, Jean Mairet R, Higuero A, et al. Protein kinase D intracellular localization and activity control kinase D-interacting substrate of 220-kDa traffic through a postsynaptic density-95/discs large/zonula occludens-1-binding motif. J Biol Chem. 2006;281:18888-900 pubmed
Yu L, Bharadwaj S, Brown J, Ma Y, Du W, Davis M, et al. Cholesterol-regulated translocation of NPC1L1 to the cell surface facilitates free cholesterol uptake. J Biol Chem. 2006;281:6616-24 pubmed
Rilla K, Siiskonen H, Spicer A, Hyttinen J, Tammi M, Tammi R. Plasma membrane residence of hyaluronan synthase is coupled to its enzymatic activity. J Biol Chem. 2005;280:31890-7 pubmed
Rajagopal R, Chen Z, Lee F, Chao M. Transactivation of Trk neurotrophin receptors by G-protein-coupled receptor ligands occurs on intracellular membranes. J Neurosci. 2004;24:6650-8 pubmed
Ortegren U, Karlsson M, Blazic N, Blomqvist M, Nystrom F, Gustavsson J, et al. Lipids and glycosphingolipids in caveolae and surrounding plasma membrane of primary rat adipocytes. Eur J Biochem. 2004;271:2028-36 pubmed
Thomas D, Weng N, Groblewski G. Secretagogue-induced translocation of CRHSP-28 within an early apical endosomal compartment in acinar cells. Am J Physiol Gastrointest Liver Physiol. 2004;287:G253-63 pubmed
Kuliawat R, Kalinina E, Bock J, Fricker L, McGraw T, Kim S, et al. Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells. Mol Biol Cell. 2004;15:1690-701 pubmed
Malosio M, Giordano T, Laslop A, Meldolesi J. Dense-core granules: a specific hallmark of the neuronal/neurosecretory cell phenotype. J Cell Sci. 2004;117:743-9 pubmed
Puri S, Telfer H, Velliste M, Murphy R, Linstedt A. Dispersal of Golgi matrix proteins during mitotic Golgi disassembly. J Cell Sci. 2004;117:451-6 pubmed
Guo Y, Martinez Williams C, Rannels D. Gap junction-microtubule associations in rat alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2003;285:L1213-21 pubmed
Ivanov A, Nusrat A, Parkos C. Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment. Mol Biol Cell. 2004;15:176-88 pubmed
Gusarova V, Brodsky J, Fisher E. Apolipoprotein B100 exit from the endoplasmic reticulum (ER) is COPII-dependent, and its lipidation to very low density lipoprotein occurs post-ER. J Biol Chem. 2003;278:48051-8 pubmed
Drecktrah D, Chambers K, Racoosin E, Cluett E, Gucwa A, Jackson B, et al. Inhibition of a Golgi complex lysophospholipid acyltransferase induces membrane tubule formation and retrograde trafficking. Mol Biol Cell. 2003;14:3459-69 pubmed
Gillingham A, Pfeifer A, Munro S. CASP, the alternatively spliced product of the gene encoding the CCAAT-displacement protein transcription factor, is a Golgi membrane protein related to giantin. Mol Biol Cell. 2002;13:3761-74 pubmed
Tran K, Thorne Tjomsland G, DeLong C, Cui Z, Shan J, Burton L, et al. Intracellular assembly of very low density lipoproteins containing apolipoprotein B100 in rat hepatoma McA-RH7777 cells. J Biol Chem. 2002;277:31187-200 pubmed
Moreno R, Schatten G, Ramalho Santos J. Golgi apparatus dynamics during mouse oocyte in vitro maturation: effect of the membrane trafficking inhibitor brefeldin A. Biol Reprod. 2002;66:1259-66 pubmed
Eystathioy T, Chan E, Tenenbaum S, Keene J, Griffith K, Fritzler M. A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles. Mol Biol Cell. 2002;13:1338-51 pubmed
Leem J, Vijayan S, Han P, Cai D, Machura M, Lopes K, et al. Presenilin 1 is required for maturation and cell surface accumulation of nicastrin. J Biol Chem. 2002;277:19236-40 pubmed
Stawowy P, Blaschke F, Kilimnik A, Goetze S, Kallisch H, Chretien M, et al. Proprotein convertase PC5 regulation by PDGF-BB involves PI3-kinase/p70(s6)-kinase activation in vascular smooth muscle cells. Hypertension. 2002;39:399-404 pubmed
Sweeney D, Siddhanta A, Shields D. Fragmentation and re-assembly of the Golgi apparatus in vitro. A requirement for phosphatidic acid and phosphatidylinositol 4,5-bisphosphate synthesis. J Biol Chem. 2002;277:3030-9 pubmed
Venkatesan S, Petrovic A, Locati M, Kim Y, Weissman D, Murphy P. A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression. J Biol Chem. 2001;276:40133-45 pubmed
Baertschi A, Monnier D, Schmidt U, Levitan E, Fakan S, Roatti A. Acid prohormone sequence determines size, shape, and docking of secretory vesicles in atrial myocytes. Circ Res. 2001;89:E23-9 pubmed
Clark A, Steely H, Dickerson J, English Wright S, Stropki K, McCartney M, et al. Glucocorticoid induction of the glaucoma gene MYOC in human and monkey trabecular meshwork cells and tissues. Invest Ophthalmol Vis Sci. 2001;42:1769-80 pubmed
Gkantiragas I, Brugger B, Stüven E, Kaloyanova D, Li X, Lohr K, et al. Sphingomyelin-enriched microdomains at the Golgi complex. Mol Biol Cell. 2001;12:1819-33 pubmed
Pixley F, Lee P, Condeelis J, Stanley E. Protein tyrosine phosphatase phi regulates paxillin tyrosine phosphorylation and mediates colony-stimulating factor 1-induced morphological changes in macrophages. Mol Cell Biol. 2001;21:1795-809 pubmed
Too C, Vickaryous N, Boudreau R, Sangster S. Identification and nuclear localization of a novel prolactin and cytokine-responsive carboxypeptidase D. Endocrinology. 2001;142:1357-67 pubmed
Stephens D, Banting G. Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin. J Biol Chem. 1999;274:30080-6 pubmed
Hu T, Kao C, Hudson R, Chen A, Draper R. Inhibition of secretion by 1,3-Cyclohexanebis(methylamine), a dibasic compound that interferes with coatomer function. Mol Biol Cell. 1999;10:921-33 pubmed
Schaefer M, Hopkins R, Failla M, Gitlin J. Hepatocyte-specific localization and copper-dependent trafficking of the Wilson's disease protein in the liver. Am J Physiol. 1999;276:G639-46 pubmed 出版商
Greenfield J, Tsai J, Gouras G, Hai B, Thinakaran G, Checler F, et al. Endoplasmic reticulum and trans-Golgi network generate distinct populations of Alzheimer beta-amyloid peptides. Proc Natl Acad Sci U S A. 1999;96:742-7 pubmed
Roquemore E, Banting G. Efficient trafficking of TGN38 from the endosome to the trans-Golgi network requires a free hydroxyl group at position 331 in the cytosolic domain. Mol Biol Cell. 1998;9:2125-44 pubmed
Reaves B, Banting G. Vacuolar ATPase inactivation blocks recycling to the trans-Golgi network from the plasma membrane. FEBS Lett. 1994;345:61-6 pubmed
Horn M, Banting G. Okadaic acid treatment leads to a fragmentation of the trans-Golgi network and an increase in expression of TGN38 at the cell surface. Biochem J. 1994;301 ( Pt 1):69-73 pubmed
Luzio J, Brake B, Banting G, Howell K, Braghetta P, Stanley K. Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38). Biochem J. 1990;270:97-102 pubmed
图像
图像 1 :
赛默飞世尔 MA3-063 图像 1
Immunofluorescent analysis of Trans Golgi Network 38 using anti-Trans Golgi Network 38 monoclonal antibody (Product# MA3-063) shows staining in NS-1 Cells.
图像 2 :
赛默飞世尔 MA3-063 图像 2
Immunofluorescent analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody (Product # MA3-063) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody (Product # 35518) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
图像 3 :
赛默飞世尔 MA3-063 图像 3
HeLa and COS7 cells were rinsed with PBS and fixed with -20°C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody (MA3-063) diluted 1:100 in PBS containing 0.2%BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. Data courtesy of the Innovators Program.
产品信息
产品类型 :
抗体
产品名称 :
TGN46单克隆抗体(2F7.1)
目录# :
MA3-063
规格 :
100微克
价格 :
美国399.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
Human, Non-human primate, Rat
应用 :
Flow Cytometry: 1/20 - 1/50, Immunocytochemistry: 1:1,000, Western Blot: 1:500
物种 :
Human, Non-human primate, Rat
克隆 :
2F7.1
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1. and ASPP2. are activators of p53.
免疫原 :
Synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
格式 :
液体
应用w/稀释 :
Flow Cytometry: 1/20 - 1/50, Immunocytochemistry: 1:1,000, Western Blot: 1:500
别名 :
AU015504; D6Ertd384e; hTGN48; hTGN51; Tgn38; TGN38 homolog; TGN38A; TGN38B; TGN41; TGN46; TGN48; TGN51; Tgoln1; Tgoln2; Trans-Golgi network glycoprotein 46; Trans-Golgi network glycoprotein 48; Trans-Golgi network glycoprotein 51; Trans-Golgi network integral membrane protein 1; trans-Golgi network integral membrane protein 2; Trans-Golgi network integral membrane protein TGN38; trans-golgi network protein; trans-Golgi network protein (46, 48, 51kD isoforms); trans-golgi network protein 1; trans-golgi network protein 2; trans-Golgi network protein TGN51; Ttgn1; TTGN2
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国