产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
雌激素受体α单克隆抗体(EVG F9)
目录 :
MA3-310
规格 :
100µL
价格 :
美国299.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
EVG F9
反应物种 :
人类,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 5
出版应用/物种/样品/稀释参考文献
  • 免疫组化-石蜡切片; 兔; 1:150; 表 1
Hernández Aragón L, García Villamar V, Carrasco Ruiz M, Nicolás Toledo L, Ortega A, Cuevas Romero E, et al. Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits. Biomed Res Int. 2017;2017:2089645 pubmed 出版商
  • 免疫组化; 兔
de los Ángeles Carrasco Ruiz M, García Villamar V, López García K, Sánchez García O, Pacheco P, Cuevas E, et al. Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits. Cell Biochem Funct. 2015;33:188-95 pubmed 出版商
  • 免疫印迹; 兔; 1:1000
Yang X, Chen G, Papp R, DeFranco D, Zeng F, Salama G. Oestrogen upregulates L-type Ca²? channels via oestrogen-receptor- by a regional genomic mechanism in female rabbit hearts. J Physiol. 2012;590:493-508 pubmed 出版商
Pavao M, Huang Y, Hafer L, Moreland R, Traish A. Immunodetection of nmt55/p54nrb isoforms in human breast cancer. BMC Cancer. 2001;1:15 pubmed
Joel P, Traish A, Lannigan D. Estradiol-induced phosphorylation of serine 118 in the estrogen receptor is independent of p42/p44 mitogen-activated protein kinase. J Biol Chem. 1998;273:13317-23 pubmed
图像
图像 1 :
赛默飞世尔 MA3-310 图像 1
Immunofluorescent analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) (Product# MA3-310) shows staining in Hela Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha (Product# MA3-310) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
雌激素受体α单克隆抗体(EVG F9)
目录# :
MA3-310
规格 :
100µL
价格 :
美国299.00
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
人类, 兔
应用 :
Immunocytochemistry: 1:100-1:200, Immunofluorescence: 1:100 -1:200, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay dependent, Western Blot: 1:500 - 1:2000
物种 :
人类, 兔
克隆 :
EVG F9
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
免疫原 :
Mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) & E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha.
格式 :
Liquid
应用w/稀释 :
Immunocytochemistry: 1:100-1:200, Immunofluorescence: 1:100 -1:200, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay dependent, Western Blot: 1:500 - 1:2000
别名 :
ER甲, ER, 雌激素受体, ESR1
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国