产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
CRMP5单克隆抗体(CR-3)
目录 :
MA3-700
规格 :
100微升
价格 :
美国375.00
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
CR-3
反应物种 :
人类, 小鼠, 大鼠, 牛
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 1
| 参考文献 |
|---|
Cross S, Salomao D, Parisi J, Kryzer T, Bradley E, Mines J, et al. Paraneoplastic autoimmune optic neuritis with retinitis defined by CRMP-5-IgG. Ann Neurol. 2003;54:38-50 pubmed
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图像
图像 1 :
Immunohistochemistry was performed on biopsies of deparaffinized Human glioma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a rat monoclonal antibody recognizing CRMP5 (MA3-700) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
图像 2 :
Immunohistochemistry was performed on biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a rat monoclonal antibody recognizing CRMP5 (MA3-700) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
CRMP5单克隆抗体(CR-3)
目录# :
MA3-700
规格 :
100微升
价格 :
美国375.00
克隆性 :
单克隆
宿主 :
大鼠
反应物种 :
牛, 人类, 小鼠, 大鼠
应用 :
Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:50 - 1:400, Western Blot: 1:50-1:1,000
物种 :
牛, 人类, 小鼠, 大鼠
克隆 :
CR-3
抗体亚型 :
IgG2a
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
The collapsin response mediator protein (CRMP) family of proteins (also referred to as ULIP6) are a group of neurologic proteins that have been found to play key roles in growth cone guidance during neural development. CRMP5 has relatively low sequence homology with the other four members of the CRMP family. CRMP5 is expressed in the developing nervous system in an expression pattern that resembles CRMP2 and has a peak expression during the first postnatal week. Elevated levels of autoantibody to CRMP5 have been shown to be linked to paraneoplastic autoimmune optic neuritis and other paraneoplastic neoplasms. Auto-immunity against CRMP5 expression (predominantly the N-terminal epitopes) is also being investigated as an oncological marker in the respiratory system and in the thymus.
免疫原 :
CRMP5蛋白纯化来自人类大脑
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:50 - 1:400, Western Blot: 1:50-1:1,000
别名 :
collapsin response mediator protein 5; collapsin response mediator protein-5; CRAM; CRMP3-associated molecule; Crmp5; CRMP-5; dihydropyrimidinase like 5; dihydropyrimidinase-like 5; dihydropyrimidinase-related protein 5; Dpysl5; DRP-5; Ulip6; ULIP-6; ULIP6 protein; UNC33-like phosphoprotein 6
更多信息或购买 :
公司信息
赛默飞世尔