产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
弹性蛋白单克隆抗体(J144)
目录 :
MA3-745
规格 :
200微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
J144
反应物种 :
人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, 其他
更多信息或购买 :
文章摘录数: 13
| 出版应用/物种/样本/稀释 | 参考文献 |
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| Sander T, Ou J, Densmore J, Kaul S, Matus I, Twigger S, et al. Protein composition of plasminogen activator inhibitor type 1-derived endothelial microparticles. Shock. 2008;29:504-11 pubmed
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| Gramolini A, Kislinger T, Alikhani Koopaei R, Fong V, Thompson N, Isserlin R, et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008;7:519-33 pubmed
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He X, Qian Y, Cai H, Yang S, Cai J, Wang Z. RhoC is essential in TGF-β1 induced epithelial-mesenchymal transition in cervical cancer cells. Oncol Lett. 2015;10:985-989 pubmed
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Takenouchi H, Kiyokawa N, Taguchi T, Matsui J, Katagiri Y, Okita H, et al. Shiga toxin binding to globotriaosyl ceramide induces intracellular signals that mediate cytoskeleton remodeling in human renal carcinoma-derived cells. J Cell Sci. 2004;117:3911-22 pubmed
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图像
图像 1 :
Immunofluorescent analysis of Vimentin using Vimentin Monoclonal antibody (J144) (Product# MA3-745) shows staining in HEK293 cells. Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin (Product# MA3-745) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
图像 2 :
Immunohistochemistry was performed on biopsies of deparaffinized Human melanoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Vimentin (MA3-745) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
图像 3 :
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Vimentin (MA3-745) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
产品信息
产品类型 :
抗体
产品名称 :
弹性蛋白单克隆抗体(J144)
目录# :
MA3-745
规格 :
200微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
狗, 人类, 小鼠, 大鼠
应用 :
Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1:1,000
物种 :
狗, 人类, 小鼠, 大鼠
克隆 :
J144
抗体亚型 :
IgM
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
免疫原 :
Human rhabdosarcoma cell line JR1.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1:1,000
别名 :
cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
更多信息或购买 :
公司信息
赛默飞世尔