产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
多聚(ADP-核糖)聚合酶单克隆抗体(C-2-10)
目录 :
MA3-950
规格 :
50µL
价格 :
美国390
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
C-2-10
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 9
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类
Jelinic P, Levine D. New insights into PARP inhibitors' effect on cell cycle and homology-directed DNA damage repair. Mol Cancer Ther. 2014;13:1645-54 pubmed 出版商
  • 免疫印迹; 人类
Lin Y, Chen Y, Cohen S, Cheng T. Identification of TSG101 functional domains and p21 loci required for TSG101-mediated p21 gene regulation. PLoS ONE. 2013;8:e79674 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:1500
  • 免疫印迹; 人类; 1:1500
Lonskaya I, Hebron M, Algarzae N, Desforges N, Moussa C. Decreased parkin solubility is associated with impairment of autophagy in the nigrostriatum of sporadic Parkinson's disease. Neuroscience. 2013;232:90-105 pubmed 出版商
  • 免疫印迹; 人类
Wu X, Ellmann S, Rubin E, Gil M, Jin K, Han L, et al. ADP ribosylation by PARP-1 suppresses HOXB7 transcriptional activity. PLoS ONE. 2012;7:e40644 pubmed 出版商
  • 免疫印迹; 人类
Yung T, Narita T, Komori T, Yamaguchi Y, Handa H. Cellular dynamics of the negative transcription elongation factor NELF. Exp Cell Res. 2009;315:1693-705 pubmed 出版商
  • 免疫印迹; 人类
Komori T, Inukai N, Yamada T, Yamaguchi Y, Handa H. Role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis. Genes Cells. 2009;14:343-54 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 1A
Zhu C, Wang X, Huang Z, Qiu L, Xu F, Vahsen N, et al. Apoptosis-inducing factor is a major contributor to neuronal loss induced by neonatal cerebral hypoxia-ischemia. Cell Death Differ. 2007;14:775-84 pubmed
  • 免疫印迹; 人类; 图 2
Duensing A, Medeiros F, McConarty B, Joseph N, Panigrahy D, Singer S, et al. Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs). Oncogene. 2004;23:3999-4006 pubmed
Mao H, Lockyer P, Townley Tilson W, Xie L, Pi X. LRP1 Regulates Retinal Angiogenesis by Inhibiting PARP-1 Activity and Endothelial Cell Proliferation. Arterioscler Thromb Vasc Biol. 2016;36:350-60 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA3-950 图像 1
Immunofluorescent analysis of PARP in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PARP monoclonal antibody (Product # MA3-950) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). PARP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
图像 2 :
赛默飞世尔 MA3-950 图像 2
Immunofluorescent analysis of PARP in HT-29 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PARP monoclonal antibody (Product # MA3-950) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). PARP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
产品信息
产品类型 :
抗体
产品名称 :
多聚(ADP-核糖)聚合酶单克隆抗体(C-2-10)
目录# :
MA3-950
规格 :
50µL
价格 :
美国390
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
Bovine, Hamster, Human, Mouse, Non-human primate, Rat
应用 :
ELISA: Assay dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:500
物种 :
Bovine, Hamster, Human, Mouse, Non-human primate, Rat
克隆 :
C-2-10
抗体亚型 :
IgG1
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
免疫原 :
Purified calf thymus poly (ADP-ribose) polymerase.
格式 :
液体
应用w/稀释 :
ELISA: Assay dependent, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:500
别名 :
5830444G22Rik; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; BOS_15645; C80510; DNA ADP-ribosyltransferase PARP1; I79_014850; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; pADPRT-1; Parp; PARP1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly ADP-ribose polymerase; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1; sPARP-1
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国