产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
乙酰组蛋白H3(Lys9)单克隆抗体(J.924.2)
目录 :
MA5-11195
规格 :
100µL
价格 :
美国345
克隆性 :
单克隆
宿主 :
共轭标签 :
未共轭
克隆名称 :
J.924.2
反应物种 :
common tobacco, 人类, 小鼠, 大鼠, 斑马鱼, 秀丽隐杆线虫
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 1
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; common tobacco; 1:200; 图 2
Mursalimov S, Permyakova N, Deineko E, Houben A, Demidov D. Cytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes. Front Plant Sci. 2015;6:846 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA5-11195 图像 1
Immunoprecipitation of acetylated Histone H3 (Lys9) was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of the indicated lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-11195) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).
产品信息
产品类型 :
抗体
产品名称 :
乙酰组蛋白H3(Lys9)单克隆抗体(J.924.2)
目录# :
MA5-11195
规格 :
100µL
价格 :
美国345
克隆性 :
单克隆
纯度 :
亲和色谱
宿主 :
反应物种 :
C. elegans, Human, Mouse, Non-human primate, Rat, Yeast, Zebrafish
应用 :
Flow Cytometry: 1:200, Immunocytochemistry: 1:400, Immunofluorescence: 1:400, Immunohistochemistry (Paraffin): 1:800, Immunoprecipitation: 1:25, Western Blot: 1:1000
物种 :
C. elegans, Human, Mouse, Non-human primate, Rat, Yeast, Zebrafish
克隆 :
J.924.2
抗体亚型 :
IgG
储存 :
-20°C
描述 :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
免疫原 :
Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated
格式 :
液体
应用w/稀释 :
Flow Cytometry: 1:200, Immunocytochemistry: 1:400, Immunofluorescence: 1:400, Immunohistochemistry (Paraffin): 1:800, Immunoprecipitation: 1:25, Western Blot: 1:1000
别名 :
H3K9ac, 组蛋白H3, 组蛋白1, H3a, 组蛋白H3.1, 组蛋白H3/a, HIST1H3A
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国