产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
雌激素受体α单克隆抗体(AER314)
目录 :
MA5-12328
规格 :
500µL
价格 :
美国309.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
AER314
反应物种 :
人类, 小鼠, 大鼠, ,
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, EMSA, 染色质免疫沉淀 , 抑制或激活实验, 免疫印迹基因敲除验证
更多信息或购买 :
文章摘录数: 14
出版应用/物种/样品/稀释参考文献
  • 免疫印迹; 人类; 图 s8
Yoshimaru T, Komatsu M, Matsuo T, Chen Y, Murakami Y, Mizuguchi K, et al. Targeting BIG3-PHB2 interaction to overcome tamoxifen resistance in breast cancer cells. Nat Commun. 2013;4:2443 pubmed 出版商
  • EMSA; 人类
Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Mason C, Shu F, Wang C, Session R, Kallen R, Sidell N, et al. Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res. 2010;38:2355-68 pubmed 出版商
  • 免疫印迹; 小鼠
Milanesi L, Vasconsuelo A, de Boland A, Boland R. Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue. Steroids. 2009;74:489-97 pubmed 出版商
  • 抑制或激活实验; 小鼠
  • 免疫细胞化学; 小鼠; 1:50
  • 免疫印迹; 小鼠; 1:400
Milanesi L, Russo de Boland A, Boland R. Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line. J Cell Biochem. 2008;104:1254-73 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Milne T, Martin M, Brock H, Slany R, Hess J. Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications. Cancer Res. 2005;65:11367-74 pubmed
  • 免疫印迹基因敲除验证; 人类; 图 4
Park W, Choi J, Hwang E, Lee J. Identification of a variant estrogen receptor lacking exon 4 and its coexpression with wild-type estrogen receptor in ovarian carcinomas. Clin Cancer Res. 1996;2:2029-35 pubmed
Laganiere J, Deblois G, Giguere V. Nuclear receptor target gene discovery using high-throughput chromatin immunoprecipitation. Methods Enzymol. 2003;364:339-50 pubmed
Thomas P, Risinger K, Klinge C. Identification of estrogen receptor beta expression in Chinese hamster ovary (CHO) cells and comparison of estrogen-responsive gene transcription in cells adapted to serum-free media. J Steroid Biochem Mol Biol. 2003;86:41-55 pubmed
Kimmins S, Russell G, Lim H, Hall B, Maclaren L. The effects of estrogen, its antagonist ICI 182, 780, and interferon-tau on the expression of estrogen receptors and integrin alphaV beta 3 on cycle day 16 in bovine endometrium. Reprod Biol Endocrinol. 2003;1:38 pubmed
Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, et al. Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition. Biol Reprod. 2002;66:976-82 pubmed
Monje P, Zanello S, Holick M, Boland R. Differential cellular localization of estrogen receptor alpha in uterine and mammary cells. Mol Cell Endocrinol. 2001;181:117-29 pubmed
Feng W, Graumann K, Hahn R, Jungbauer A. Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography. J Chromatogr A. 1999;852:161-73 pubmed
Biswas D, Averboukh L, Sheng S, Martin K, Ewaniuk D, Jawde T, et al. Classification of breast cancer cells on the basis of a functional assay for estrogen receptor. Mol Med. 1998;4:454-67 pubmed
图像
图像 1 :
赛默飞世尔 MA5-12328 图像 1
图像 2 :
赛默飞世尔 MA5-12328 图像 2
产品信息
产品类型 :
抗体
产品名称 :
雌激素受体α单克隆抗体(AER314)
目录# :
MA5-12328
规格 :
500µL
价格 :
美国309.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
牛, 人类, 小鼠, 兔, 大鼠
应用 :
Gel Shift: 1mg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1:100-1:400
物种 :
牛, 人类, 小鼠, 兔, 大鼠
克隆 :
AER314
抗体亚型 :
IgG1, lambda
储存 :
4° C
描述 :
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
免疫原 :
Purified, SDS-denatured calf uterus estrogen receptor (ER)
格式 :
Liquid
应用w/稀释 :
Gel Shift: 1mg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1:100-1:400
别名 :
ER-alpha, estradiol receptor, estrogen receptor, estrogen receptor alpha delta 3*,4,5,6,7*/819-2 isoform, estrogen receptor alpha delta 4 +49 isoform, estrogen receptor alpha delta 4*,5,6,7*/654 isoform, nuclear receptor subfamily 3 group A member 1
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国