产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
p53单克隆抗体(DO-1)
目录 :
MA5-12571
规格 :
500微升
价格 :
美国375.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
DO-1
反应物种 :
African green monkey, 人类, 小鼠, 猕猴
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 染色质免疫沉淀 , 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 78
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 4a
Leslie P, Franklin D, Liu Y, Zhang Y. p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop. Cell Rep. 2018;24:1484-1495 pubmed 出版商
  • 免疫印迹; 人类; 图 1c
Bernal A, Moltó Abad M, Dominguez D, Tusell L. Acute telomere deprotection prevents ongoing BFB cycles and rampant instability in p16INK4a-deficient epithelial cells. Oncotarget. 2018;9:27151-27170 pubmed 出版商
  • 免疫组化-冰冻切片; 人类; 1:100; 图 1a
Pommier A, Anaparthy N, Memos N, Kelley Z, Gouronnec A, Yan R, et al. Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases. Science. 2018;360: pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 图 2
Walline H, Carey T, Goudsmit C, Bellile E, D Souza G, Peterson L, et al. High-Risk HPV, Biomarkers, and Outcome in Matched Cohorts of Head and Neck Cancer Patients Positive and Negative for HIV. Mol Cancer Res. 2017;15:179-188 pubmed 出版商
  • 免疫印迹; 人类; 图 2a
Liu X, Wang S, Guo X, Wei F, Yin J, Zang Y, et al. Exogenous p53 and ASPP2 expression enhances rAdV-TK/ GCV-induced death in hepatocellular carcinoma cells lacking functional p53. Oncotarget. 2016;7:18896-905 pubmed 出版商
  • 免疫印迹; 人类; 图 s2a
Gruosso T, Garnier C, Abélanet S, Kieffer Y, Lemesre V, Bellanger D, et al. MAP3K8/TPL-2/COT is a potential predictive marker for MEK inhibitor treatment in high-grade serous ovarian carcinomas. Nat Commun. 2015;6:8583 pubmed 出版商
  • 免疫组化; 人类; 1:200; 图 3
Catenacci D, Chapman C, Xu P, Koons A, Konda V, Siddiqui U, et al. Acquisition of Portal Venous Circulating Tumor Cells From Patients With Pancreaticobiliary Cancers by Endoscopic Ultrasound. Gastroenterology. 2015;149:1794-1803.e4 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Leslie P, Ke H, Zhang Y. The MDM2 RING domain and central acidic domain play distinct roles in MDM2 protein homodimerization and MDM2-MDMX protein heterodimerization. J Biol Chem. 2015;290:12941-50 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100
Beck A, Brooks A, Zeiss C. Invasive ductular carcinoma in 2 rhesus macaques (Macaca mulatta). Comp Med. 2014;64:314-22 pubmed
  • 免疫印迹; 人类; 1:1000
Senkiv Y, Riabtseva A, Heffeter P, Boiko N, Kowol C, Jungwith U, et al. Enhanced anticancer activity and circumvention of resistance mechanisms by novel polymeric/ phospholipidic nanocarriers of doxorubicin. J Biomed Nanotechnol. 2014;10:1369-81 pubmed
  • 免疫组化; 人类; 1:100
Saglam O, Dai F, Husain S, Zhan Y, Toruner G, Haines G. Matricellular protein CCN1 (CYR61) expression is associated with high-grade ductal carcinoma in situ. Hum Pathol. 2014;45:1269-75 pubmed 出版商
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Yan S, Qiu L, Ma K, Zhang X, Zhao Y, Zhang J, et al. FATS is an E2-independent ubiquitin ligase that stabilizes p53 and promotes its activation in response to DNA damage. Oncogene. 2014;33:5424-33 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100
  • 免疫组化; 人类; 1:100
Bradford C, Kumar B, Bellile E, Lee J, Taylor J, D SILVA N, et al. Biomarkers in advanced larynx cancer. Laryngoscope. 2014;124:179-87 pubmed 出版商
  • 免疫印迹; 人类; 1:1000
Dornetshuber Fleiss R, Heffeter P, Mohr T, Hazemi P, Kryeziu K, Seger C, et al. Destruxins: fungal-derived cyclohexadepsipeptides with multifaceted anticancer and antiangiogenic activities. Biochem Pharmacol. 2013;86:361-77 pubmed 出版商
  • 免疫印迹; 人类
Cheng J, Rozenblatt Rosen O, Paulson K, Nghiem P, DeCaprio J. Merkel cell polyomavirus large T antigen has growth-promoting and inhibitory activities. J Virol. 2013;87:6118-26 pubmed 出版商
  • 免疫印迹; 人类; 1:1000
Valiahdi S, Egger A, Miklos W, Jungwirth U, Meelich K, Nock P, et al. Influence of extracellular pH on the cytotoxicity, cellular accumulation, and DNA interaction of novel pH-sensitive 2-aminoalcoholatoplatinum(II) complexes. J Biol Inorg Chem. 2013;18:249-60 pubmed 出版商
  • 免疫印迹; 人类
Durando M, Tateishi S, Vaziri C. A non-catalytic role of DNA polymerase η in recruiting Rad18 and promoting PCNA monoubiquitination at stalled replication forks. Nucleic Acids Res. 2013;41:3079-93 pubmed 出版商
  • 免疫印迹; 人类
Barrasa J, Santiago Gómez A, Olmo N, Lizarbe M, Turnay J. Resistance to butyrate impairs bile acid-induced apoptosis in human colon adenocarcinoma cells via up-regulation of Bcl-2 and inactivation of Bax. Biochim Biophys Acta. 2012;1823:2201-9 pubmed 出版商
  • 免疫印迹; 小鼠
Clegg H, Itahana Y, Itahana K, Ramalingam S, Zhang Y. Mdm2 RING mutation enhances p53 transcriptional activity and p53-p300 interaction. PLoS ONE. 2012;7:e38212 pubmed 出版商
  • 免疫组化; 人类; 1:100
Eliassen A, Hauff S, Tang A, Thomas D, McHugh J, Walline H, et al. Head and neck squamous cell carcinoma in pregnant women. Head Neck. 2013;35:335-42 pubmed 出版商
  • 免疫印迹; 人类
Preusser M, Spiegl Kreinecker S, Lötsch D, Wöhrer A, Schmook M, Dieckmann K, et al. Trabectedin has promising antineoplastic activity in high-grade meningioma. Cancer. 2012;118:5038-49 pubmed 出版商
  • 免疫印迹; 人类; 1:1000
Muenyi C, Pinhas A, Fan T, Brock G, Helm C, States J. Sodium arsenite ± hyperthermia sensitizes p53-expressing human ovarian cancer cells to cisplatin by modulating platinum-DNA damage responses. Toxicol Sci. 2012;127:139-49 pubmed 出版商
  • 免疫印迹; 人类
Ignacio Barrasa J, Olmo N, Pérez Ramos P, Santiago Gómez A, Lecona E, Turnay J, et al. Deoxycholic and chenodeoxycholic bile acids induce apoptosis via oxidative stress in human colon adenocarcinoma cells. Apoptosis. 2011;16:1054-67 pubmed 出版商
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Deisenroth C, Itahana Y, Tollini L, Jin A, Zhang Y. p53-Inducible DHRS3 is an endoplasmic reticulum protein associated with lipid droplet accumulation. J Biol Chem. 2011;286:28343-56 pubmed 出版商
  • 免疫印迹; 人类
Pirker C, Lötsch D, Spiegl Kreinecker S, Jantscher F, Sutterlüty H, Micksche M, et al. Response of experimental malignant melanoma models to the pan-Aurora kinase inhibitor VE-465. Exp Dermatol. 2010;19:1040-7 pubmed 出版商
  • 免疫印迹; 小鼠
Macias E, Jin A, Deisenroth C, Bhat K, Mao H, Lindström M, et al. An ARF-independent c-MYC-activated tumor suppression pathway mediated by ribosomal protein-Mdm2 Interaction. Cancer Cell. 2010;18:231-43 pubmed 出版商
  • 免疫细胞化学; 小鼠
Ito T, Kwon H, Zimdahl B, Congdon K, Blum J, Lento W, et al. Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature. 2010;466:765-8 pubmed 出版商
  • 免疫印迹; 人类
Deisenroth C, Thorner A, Enomoto T, Perou C, Zhang Y. Mitochondrial Hep27 is a c-Myb target gene that inhibits Mdm2 and stabilizes p53. Mol Cell Biol. 2010;30:3981-93 pubmed 出版商
  • 免疫印迹; 人类
Kumar Y, Radha V, Swarup G. Interaction with Sug1 enables Ipaf ubiquitination leading to caspase 8 activation and cell death. Biochem J. 2010;427:91-104 pubmed 出版商
  • 免疫印迹; 人类
Räsänen K, Virtanen I, Salmenperä P, Grenman R, Vaheri A. Differences in the nemosis response of normal and cancer-associated fibroblasts from patients with oral squamous cell carcinoma. PLoS ONE. 2009;4:e6879 pubmed 出版商
  • 染色质免疫沉淀 ; 人类; 3 ug
  • 免疫细胞化学; 人类
Yan H, Xue G, Mei Q, Wang Y, Ding F, Liu M, et al. Repression of the miR-17-92 cluster by p53 has an important function in hypoxia-induced apoptosis. EMBO J. 2009;28:2719-32 pubmed 出版商
  • 免疫印迹; 人类; 1:500
Herberger B, Berger W, Puhalla H, Schmid K, Novak S, Brandstetter A, et al. Simultaneous blockade of the epidermal growth factor receptor/mammalian target of rapamycin pathway by epidermal growth factor receptor inhibitors and rapamycin results in reduced cell growth and survival in biliary tract cancer cells. Mol Cancer Ther. 2009;8:1547-56 pubmed 出版商
  • 免疫组化; 人类; 1:100
Bakhshi S, Gupta A, Sharma M, Khan S, Rastogi S. Her-2/neu, p-53, and their coexpression in osteosarcoma. J Pediatr Hematol Oncol. 2009;31:245-51 pubmed 出版商
  • 免疫印迹; 人类
Ulasov I, Sonabend A, Nandi S, Khramtsov A, Han Y, Lesniak M. Combination of adenoviral virotherapy and temozolomide chemotherapy eradicates malignant glioma through autophagic and apoptotic cell death in vivo. Br J Cancer. 2009;100:1154-64 pubmed 出版商
  • 免疫印迹; 人类
Ulasov I, Tyler M, Zhu Z, Han Y, He T, Lesniak M. Oncolytic adenoviral vectors which employ the survivin promoter induce glioma oncolysis via a process of beclin-dependent autophagy. Int J Oncol. 2009;34:729-42 pubmed
  • 免疫印迹; 人类
Itahana Y, Ke H, Zhang Y. p53 Oligomerization is essential for its C-terminal lysine acetylation. J Biol Chem. 2009;284:5158-64 pubmed 出版商
  • 免疫组化; 人类; 1:20
Russo G, Anzivino E, Fioriti D, Mischitelli M, Bellizzi A, Giordano A, et al. p53 gene mutational rate, Gleason score, and BK virus infection in prostate adenocarcinoma: Is there a correlation?. J Med Virol. 2008;80:2100-7 pubmed 出版商
  • 免疫印迹; 人类
Hori M, Suzuki K, Udono M, Yamauchi M, Mine M, Watanabe M, et al. Establishment of ponasterone A-inducible the wild-type p53 protein-expressing clones from HSC-1 cells, cell growth suppression by p53 expression and the suppression mechanism. Arch Dermatol Res. 2009;301:631-46 pubmed 出版商
  • 免疫印迹; 人类; 图 7
  • 免疫印迹; 小鼠; 图 7
Itahana K, Zhang Y. Mitochondrial p32 is a critical mediator of ARF-induced apoptosis. Cancer Cell. 2008;13:542-53 pubmed 出版商
  • 免疫组化; 人类
Kumar B, Cordell K, D SILVA N, Prince M, Adams M, Fisher S, et al. Expression of p53 and Bcl-xL as predictive markers for larynx preservation in advanced laryngeal cancer. Arch Otolaryngol Head Neck Surg. 2008;134:363-9 pubmed 出版商
  • 免疫细胞化学; 人类
Nishizuka S, Spurrier B. Experimental validation for quantitative protein network models. Curr Opin Biotechnol. 2008;19:41-9 pubmed 出版商
  • 免疫印迹; 人类
Krejci O, Wunderlich M, Geiger H, Chou F, Schleimer D, Jansen M, et al. p53 signaling in response to increased DNA damage sensitizes AML1-ETO cells to stress-induced death. Blood. 2008;111:2190-9 pubmed
  • 免疫组化; 人类; 1:100
Das P, Vaiphei K, Jain D, Wig J. p53 and mdm2 expression in colorectal carcinoma: a correlative analysis with clinical staging and histological parameters. Int J Surg Pathol. 2007;15:335-45 pubmed
  • 免疫组化; 人类
Bauer J, Kumar B, Cordell K, Prince M, Tran H, Wolf G, et al. Targeting apoptosis to overcome cisplatin resistance: a translational study in head and neck cancer. Int J Radiat Oncol Biol Phys. 2007;69:S106-8 pubmed
  • 免疫印迹; 人类
Skvortsov S, Skvortsova I, Stasyk T, Schiefermeier N, Neher A, Gunkel A, et al. Antitumor activity of CTFB, a novel anticancer agent, is associated with the down-regulation of nuclear factor-kappaB expression and proteasome activation in head and neck squamous carcinoma cell lines. Mol Cancer Ther. 2007;6:1898-908 pubmed
  • 流式细胞仪; 人类
Liu B, Zhang H, Zhou G, Xie Y, Hao J, Qiu R, et al. Adenovirus-mediated p53 gene transfer sensitizes hepatocellular carcinoma cells to heavy-ion radiation. J Gastroenterol. 2007;42:140-5 pubmed
  • 免疫沉淀; 小鼠
Skaar J, Florens L, Tsutsumi T, Arai T, Tron A, Swanson S, et al. PARC and CUL7 form atypical cullin RING ligase complexes. Cancer Res. 2007;67:2006-14 pubmed
  • 染色质免疫沉淀 ; 人类
  • 免疫沉淀; 人类; 1 ug/time
  • 免疫印迹; 人类
Zhu Q, Wani G, Yao J, Patnaik S, Wang Q, El Mahdy M, et al. The ubiquitin-proteasome system regulates p53-mediated transcription at p21waf1 promoter. Oncogene. 2007;26:4199-208 pubmed
  • 免疫细胞化学; 人类; 1:100
Jiang L, Ma Y, Wang J, Tao X, Wei D. The transduction of His-TAT-p53 fusion protein into the human osteogenic sarcoma cell line (Saos-2) and its influence on cell cycle arrest and apoptosis. Mol Biol Rep. 2008;35:1-8 pubmed
  • 免疫印迹; 人类
Cao F, Zhou T, Simpson D, Zhou Y, Boyer J, Chen B, et al. p53-Dependent but ATM-independent inhibition of DNA synthesis and G2 arrest in cadmium-treated human fibroblasts. Toxicol Appl Pharmacol. 2007;218:174-85 pubmed
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Luo M, Rosenke K, Czornak K, Fortunato E. Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection. J Virol. 2007;81:1934-50 pubmed
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Kasper J, Arai T, DeCaprio J. A novel p53-binding domain in CUL7. Biochem Biophys Res Commun. 2006;348:132-8 pubmed
  • 免疫印迹; 人类
Taylor B, McNeely S, Miller H, Lehmann G, McCabe M, States J. p53 suppression of arsenite-induced mitotic catastrophe is mediated by p21CIP1/WAF1. J Pharmacol Exp Ther. 2006;318:142-51 pubmed
  • 免疫沉淀; 人类
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Borger D, DeCaprio J. Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53. J Virol. 2006;80:4292-303 pubmed
  • 免疫印迹; African green monkey
Chen W, Wang D, Yen R, Luo J, Gu W, Baylin S. Tumor suppressor HIC1 directly regulates SIRT1 to modulate p53-dependent DNA-damage responses. Cell. 2005;123:437-48 pubmed
  • 免疫印迹; 人类
Kasper J, Kuwabara H, Arai T, Ali S, DeCaprio J. Simian virus 40 large T antigen's association with the CUL7 SCF complex contributes to cellular transformation. J Virol. 2005;79:11685-92 pubmed
  • 免疫印迹; 人类
Porter P, Clark D, McDaniel L, McGregor W, States J. Telomerase-immortalized human fibroblasts retain UV-induced mutagenesis and p53-mediated DNA damage responses. DNA Repair (Amst). 2006;5:61-70 pubmed
Burger K, Schlackow M, Gullerova M. Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks. Nucleic Acids Res. 2019;47:3467-3484 pubmed 出版商
Liao P, Zeng S, Zhou X, Chen T, Zhou F, Cao B, et al. Mutant p53 Gains Its Function via c-Myc Activation upon CDK4 Phosphorylation at Serine 249 and Consequent PIN1 Binding. Mol Cell. 2017;68:1134-1146.e6 pubmed 出版商
Senturk S, Shirole N, Nowak D, Corbo V, Pal D, Vaughan A, et al. Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization. Nat Commun. 2017;8:14370 pubmed 出版商
Huang Y, Chen N, Miao D. Radioprotective effects of pyrroloquinoline quinone on parotid glands in C57BL/6J mice. Exp Ther Med. 2016;12:3685-3693 pubmed 出版商
Castellini L, Moon E, Razorenova O, Krieg A, von Eyben R, Giaccia A. KDM4B/JMJD2B is a p53 target gene that modulates the amplitude of p53 response after DNA damage. Nucleic Acids Res. 2017;45:3674-3692 pubmed 出版商
Wachter F, Morgan A, Godes M, Mourtada R, Bird G, Walensky L. Mechanistic validation of a clinical lead stapled peptide that reactivates p53 by dual HDM2 and HDMX targeting. Oncogene. 2017;36:2184-2190 pubmed 出版商
Murray H, Maltby V, Smith D, Bowden N. Nucleotide excision repair deficiency in melanoma in response to UVA. Exp Hematol Oncol. 2015;5:6 pubmed 出版商
Pages M, Lacroix L, Tauziède Espariat A, Castel D, Daudigeos Dubus E, Ridola V, et al. Papillary glioneuronal tumors: histological and molecular characteristics and diagnostic value of SLC44A1-PRKCA fusion. Acta Neuropathol Commun. 2015;3:85 pubmed 出版商
Verreault M, Schmitt C, Goldwirt L, Pelton K, Haidar S, Levasseur C, et al. Preclinical Efficacy of the MDM2 Inhibitor RG7112 in MDM2-Amplified and TP53 Wild-type Glioblastomas. Clin Cancer Res. 2016;22:1185-96 pubmed 出版商
Salas E, Roy S, Marsh T, Rubin B, Debnath J. Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell death. Oncogene. 2016;35:2913-22 pubmed 出版商
O Loghlen A, Brookes S, Martin N, Rapisarda V, Peters G, Gil J. CBX7 and miR-9 are part of an autoregulatory loop controlling p16(INK) (4a). Aging Cell. 2015;14:1113-21 pubmed 出版商
Mondesert O, Frongia C, Clayton O, Boizeau M, Lobjois V, Ducommun B. Monitoring the Activation of the DNA Damage Response Pathway in a 3D Spheroid Model. PLoS ONE. 2015;10:e0134411 pubmed 出版商
Sappino A, Buser R, Seguin Q, Fernet M, Lesne L, Gumy Pause F, et al. The CEACAM1 tumor suppressor is an ATM and p53-regulated gene required for the induction of cellular senescence by DNA damage. Oncogenesis. 2012;1:e7 pubmed 出版商
Kopp F, Oak P, Wagner E, Roidl A. miR-200c sensitizes breast cancer cells to doxorubicin treatment by decreasing TrkB and Bmi1 expression. PLoS ONE. 2012;7:e50469 pubmed 出版商
Vilotti S, Codrich M, Dal Ferro M, Pinto M, Ferrer I, Collavin L, et al. Parkinson's disease DJ-1 L166P alters rRNA biogenesis by exclusion of TTRAP from the nucleolus and sequestration into cytoplasmic aggregates via TRAF6. PLoS ONE. 2012;7:e35051 pubmed 出版商
Korfali N, Srsen V, Waterfall M, Batrakou D, Pekovic V, Hutchison C, et al. A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4/Tmem53 as involved in stress-dependent cell cycle withdrawal. PLoS ONE. 2011;6:e18762 pubmed 出版商
Yamauchi M, Suzuki K, Kodama S, Watanabe M. Stabilization of alanine substituted p53 protein at Ser15, Thr18, and Ser20 in response to ionizing radiation. Biochem Biophys Res Commun. 2004;323:906-11 pubmed
Das D, Shah R, Imperiale M. Detection and expression of human BK virus sequences in neoplastic prostate tissues. Oncogene. 2004;23:7031-46 pubmed
Sharif M, Whitehouse A, Sharman P, Perry M, Adams M. Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3. Arthritis Rheum. 2004;50:507-15 pubmed
Fisher A, Ferrario A, Rucker N, Zhang S, Gomer C. Photodynamic therapy sensitivity is not altered in human tumor cells after abrogation of p53 function. Cancer Res. 1999;59:331-5 pubmed
Saito H, Ebinuma H, Takahashi M, Kaneko F, Wakabayashi K, Nakamura M, et al. Loss of butyrate-induced apoptosis in human hepatoma cell lines HCC-M and HCC-T having substantial Bcl-2 expression. Hepatology. 1998;27:1233-40 pubmed
图像
图像 1 :
赛默飞世尔 MA5-12571 图像 1
Formalin-fixed, paraffin-embedded human colon carcinoma stained with p53 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.
产品信息
产品类型 :
抗体
产品名称 :
p53单克隆抗体(DO-1)
目录# :
MA5-12571
规格 :
500微升
价格 :
美国375.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
人类, 猕猴
应用 :
ChIP assay: 1-3 µg x 10^6 cells, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1-2 µg/mL
物种 :
人类, 猕猴
克隆 :
DO-1
抗体亚型 :
IgG2a
储存 :
4° C
描述 :
The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.
免疫原 :
Recombinant human wild-type p53 protein expressed in E. coli
格式 :
液体
应用w/稀释 :
ChIP assay: 1-3 µg x 10^6 cells, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1-2 µg/mL
别名 :
Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国