产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Ku80单克隆抗体(111)
目录 :
MA5-12933
规格 :
500微升
价格 :
美国446.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
111
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪, EMSA, 抑制或激活实验
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 60
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 1:1000; 图 4d
Tay L, Krishnan V, Sankar H, Chong Y, Chuang L, Tan T, et al. RUNX Poly(ADP-Ribosyl)ation and BLM Interaction Facilitate the Fanconi Anemia Pathway of DNA Repair. Cell Rep. 2018;24:1747-1755 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1f
Zan H, Tat C, Qiu Z, Taylor J, Guerrero J, Shen T, et al. Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination. Nat Commun. 2017;8:14244 pubmed 出版商
  • 免疫沉淀; 人类; 图 1a
  • 免疫印迹; 人类
Bouley J, Saad L, Grall R, Schellenbauer A, Biard D, Paget V, et al. A new phosphorylated form of Ku70 identified in resistant leukemic cells confers fast but unfaithful DNA repair in cancer cell lines. Oncotarget. 2015;6:27980-8000 pubmed 出版商
  • 免疫印迹; 人类; 图 5
Hirakawa H, Fujisawa H, Masaoka A, Noguchi M, Hirayama R, Takahashi M, et al. The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells. Cancer Med. 2015;4:426-36 pubmed 出版商
  • 免疫细胞化学; 人类; 1:200
Coker H, Brockdorff N. SMCHD1 accumulates at DNA damage sites and facilitates the repair of DNA double-strand breaks. J Cell Sci. 2014;127:1869-74 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100; 表 2
  • 免疫印迹; 人类; 1:2000; 表 2
Britton S, Coates J, Jackson S. A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol. 2013;202:579-95 pubmed 出版商
  • EMSA; 人类
Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed 出版商
  • 免疫细胞化学; 人类; 1:400
Calkins A, Iglehart J, Lazaro J. DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1. Nucleic Acids Res. 2013;41:7378-86 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Cottarel J, Frit P, Bombarde O, Salles B, Négrel A, Bernard S, et al. A noncatalytic function of the ligation complex during nonhomologous end joining. J Cell Biol. 2013;200:173-86 pubmed 出版商
  • 免疫印迹; 人类
Qiao B, Kerr M, Groselj B, Teo M, Knowles M, Bristow R, et al. Imatinib radiosensitizes bladder cancer by targeting homologous recombination. Cancer Res. 2013;73:1611-20 pubmed 出版商
  • 免疫印迹; 人类; 1:500
Wei S, Xiong M, Zhan D, Liang B, Wang Y, Gutmann D, et al. Ku80 functions as a tumor suppressor in hepatocellular carcinoma by inducing S-phase arrest through a p53-dependent pathway. Carcinogenesis. 2012;33:538-47 pubmed 出版商
  • 免疫印迹; 人类
Bouquet F, Ousset M, Biard D, Fallone F, Dauvillier S, Frit P, et al. A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia. J Cell Sci. 2011;124:1943-51 pubmed 出版商
  • 抑制或激活实验; 人类; 200 ug/ml
  • 免疫印迹; 人类
Choi Y, Nash K, Byrne B, Muzyczka N, Song S. The effect of DNA-dependent protein kinase on adeno-associated virus replication. PLoS ONE. 2010;5:e15073 pubmed 出版商
  • 免疫印迹; 人类
Ohno M, Kunimoto M, Nishizuka M, Osada S, Imagawa M. Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression. Biochem Biophys Res Commun. 2009;390:738-42 pubmed 出版商
  • 免疫印迹; 人类; 1:200
Li S, Kuhne W, Kulharya A, Hudson F, Ha K, Cao Z, et al. Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance. Nucleic Acids Res. 2009;37:6746-53 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Quanz M, Chassoux D, Berthault N, Agrario C, Sun J, Dutreix M. Hyperactivation of DNA-PK by double-strand break mimicking molecules disorganizes DNA damage response. PLoS ONE. 2009;4:e6298 pubmed 出版商
  • 抑制或激活实验; 人类; 20 ug/ml
  • 流式细胞仪; 人类
  • 免疫印迹; 人类
Paupert J, Mansat De Mas V, Demur C, Salles B, Muller C. Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features. Cell Cycle. 2008;7:1047-53 pubmed
  • 免疫印迹; 人类
Yu D, Sekine E, Fujimori A, Ochiya T, Okayasu R. Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo. Cancer Sci. 2008;99:810-5 pubmed 出版商
  • EMSA; 人类
Lehman J, Hoelz D, Turchi J. DNA-dependent conformational changes in the Ku heterodimer. Biochemistry. 2008;47:4359-68 pubmed 出版商
  • 免疫印迹; 人类
Iwamoto F, Stadler M, Chalupníková K, Oakeley E, Nagamine Y. Transcription-dependent nucleolar cap localization and possible nuclear function of DExH RNA helicase RHAU. Exp Cell Res. 2008;314:1378-91 pubmed 出版商
  • 免疫印迹; 人类
Wu P, Frit P, Malivert L, Revy P, Biard D, Salles B, et al. Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell. J Biol Chem. 2007;282:31937-43 pubmed
  • 免疫印迹; 人类
Hegarat N, Cardoso G, Rusconi F, Francois J, Praseuth D. Analytical biochemistry of DNA--protein assemblies from crude cell extracts. Nucleic Acids Res. 2007;35:e92 pubmed
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Despras E, Pfeiffer P, Salles B, Calsou P, Kuhfittig Kulle S, Angulo J, et al. Long-term XPC silencing reduces DNA double-strand break repair. Cancer Res. 2007;67:2526-34 pubmed
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Luo M, Rosenke K, Czornak K, Fortunato E. Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection. J Virol. 2007;81:1934-50 pubmed
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Drouet J, Frit P, Delteil C, de Villartay J, Salles B, Calsou P. Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends. J Biol Chem. 2006;281:27784-93 pubmed
  • 免疫印迹; 人类
Howlett N, Scuric Z, D Andrea A, Schiestl R. Impaired DNA double strand break repair in cells from Nijmegen breakage syndrome patients. DNA Repair (Amst). 2006;5:251-7 pubmed
  • 免疫印迹; 人类
Fogal V, Hsieh J, Royer C, Zhong S, Lu X. Cell cycle-dependent nuclear retention of p53 by E2F1 requires phosphorylation of p53 at Ser315. EMBO J. 2005;24:2768-82 pubmed
  • 免疫印迹; 人类
Luk J, Su Y, Lam S, Lee C, Hu M, He Q, et al. Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma. Proteomics. 2005;5:1980-6 pubmed
  • 免疫印迹; 人类; 1:5000
Deriano L, Guipaud O, Merle Béral H, Binet J, Ricoul M, Potocki Veronese G, et al. Human chronic lymphocytic leukemia B cells can escape DNA damage-induced apoptosis through the nonhomologous end-joining DNA repair pathway. Blood. 2005;105:4776-83 pubmed
Guo G, Gao M, Gao X, Zhu B, Huang J, Tu X, et al. Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling. Nat Commun. 2021;12:2187 pubmed 出版商
Shan W, Yuan J, Hu Z, Jiang J, Wang Y, Loo N, et al. Systematic Characterization of Recurrent Genomic Alterations in Cyclin-Dependent Kinases Reveals Potential Therapeutic Strategies for Cancer Treatment. Cell Rep. 2020;32:107884 pubmed 出版商
Chakraborty S, Pandita R, Hambarde S, Mattoo A, Charaka V, Ahmed K, et al. SMARCAD1 Phosphorylation and Ubiquitination Are Required for Resection during DNA Double-Strand Break Repair. iScience. 2018;2:123-135 pubmed 出版商
Wang X, Liu H, Shi L, Yu X, Gu Y, Sun X. LINP1 facilitates DNA damage repair through non-homologous end joining (NHEJ) pathway and subsequently decreases the sensitivity of cervical cancer cells to ionizing radiation. Cell Cycle. 2018;17:439-447 pubmed 出版商
Wang Y, Xu H, Liu T, Huang M, Butter P, Li C, et al. Temporal DNA-PK activation drives genomic instability and therapy resistance in glioma stem cells. JCI Insight. 2018;3: pubmed 出版商
Zhang Y, He Q, Hu Z, Feng Y, Fan L, Tang Z, et al. Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer. Nat Struct Mol Biol. 2016;23:522-30 pubmed 出版商
Pagotto A, Caballero O, Volkmar N, Devalle S, Simpson A, Lu X, et al. Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1 is regulated by proteasome activity in tumour cells. PLoS ONE. 2013;8:e83212 pubmed 出版商
Sand Dejmek J, Adelmant G, Sobhian B, Calkins A, Marto J, Iglehart D, et al. Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT) in DNA repair, apoptosis and necrosis after cisplatin. Mol Cancer. 2011;10:74 pubmed 出版商
Shi L, Qiu D, Zhao G, Corthesy B, Lees Miller S, Reeves W, et al. Dynamic binding of Ku80, Ku70 and NF90 to the IL-2 promoter in vivo in activated T-cells. Nucleic Acids Res. 2007;35:2302-10 pubmed
Audebert M, Salles B, Calsou P. Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining. J Biol Chem. 2004;279:55117-26 pubmed
Monferran S, Paupert J, Dauvillier S, Salles B, Muller C. The membrane form of the DNA repair protein Ku interacts at the cell surface with metalloproteinase 9. EMBO J. 2004;23:3758-68 pubmed
Lau A, Kanaar R, Jackson S, O Connor M. Suppression of retroviral infection by the RAD52 DNA repair protein. EMBO J. 2004;23:3421-9 pubmed
Kim E, Edmonston C, Wu X, Schaffer A, Casali P. The HoxC4 homeodomain protein mediates activation of the immunoglobulin heavy chain 3' hs1,2 enhancer in human B cells. Relevance to class switch DNA recombination. J Biol Chem. 2004;279:42258-69 pubmed
Onclercq Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor Gueret M. Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing. Nucleic Acids Res. 2003;31:6272-82 pubmed
Partridge J, Lopreiato J, Latterich M, Indig F. DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97/VCP. Mol Biol Cell. 2003;14:4221-9 pubmed
Udayakumar D, Bladen C, Hudson F, Dynan W. Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. J Biol Chem. 2003;278:41631-5 pubmed
Calsou P, Delteil C, Frit P, Drouet J, Salles B. Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment. J Mol Biol. 2003;326:93-103 pubmed
Bertinato J, Tomlinson J, Schild Poulter C, Haché R. Evidence implicating Ku antigen as a structural factor in RNA polymerase II-mediated transcription. Gene. 2003;302:53-64 pubmed
Herbert A, Wagner S, Nickerson J. Induction of protein translation by ADAR1 within living cell nuclei is not dependent on RNA editing. Mol Cell. 2002;10:1235-46 pubmed
Ader I, Muller C, Bonnet J, Favre G, Cohen Jonathan E, Salles B, et al. The radioprotective effect of the 24 kDa FGF-2 isoform in HeLa cells is related to an increased expression and activity of the DNA dependent protein kinase (DNA-PK) catalytic subunit. Oncogene. 2002;21:6471-9 pubmed
Ma Y, Pannicke U, Schwarz K, Lieber M. Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell. 2002;108:781-94 pubmed
Ma Y, Lieber M. Binding of inositol hexakisphosphate (IP6) to Ku but not to DNA-PKcs. J Biol Chem. 2002;277:10756-9 pubmed
Jafri F, Hardin J, Dynan W. A method to detect particle-specific antibodies against Ku and the DNA-dependent protein kinase catalytic subunit in autoimmune sera. J Immunol Methods. 2001;251:53-61 pubmed
Lynch E, Moreland R, Ginis I, Perrine S, Faller D. Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro. Am J Physiol Cell Physiol. 2001;280:C897-911 pubmed
Bertinato J, Schild Poulter C, Hache R. Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits. J Cell Sci. 2001;114:89-99 pubmed
Frit P, Li R, Arzel D, Salles B, Calsou P. Ku entry into DNA inhibits inward DNA transactions in vitro. J Biol Chem. 2000;275:35684-91 pubmed
Chen L, Trujillo K, Sung P, Tomkinson A. Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase. J Biol Chem. 2000;275:26196-205 pubmed
Galande S, Kohwi Shigematsu T. Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences. J Biol Chem. 1999;274:20521-8 pubmed
Zhang Y, Xiong Y. Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53. Mol Cell. 1999;3:579-91 pubmed
Giffin W, Gong W, Schild Poulter C, Hache R. Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription. Mol Cell Biol. 1999;19:4065-78 pubmed
Camara Clayette V, Thomas D, Rahuel C, Barbey R, Cartron J, Bertrand O. The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit. Nucleic Acids Res. 1999;27:1656-63 pubmed
图像
图像 1 :
赛默飞世尔 MA5-12933 图像 1
图像 2 :
赛默飞世尔 MA5-12933 图像 2
图像 3 :
赛默飞世尔 MA5-12933 图像 3
产品信息
产品类型 :
抗体
产品名称 :
Ku80单克隆抗体(111)
目录# :
MA5-12933
规格 :
500微升
价格 :
美国446.00
克隆性 :
单克隆
纯度 :
硫酸铵沉淀
宿主 :
小鼠
反应物种 :
Human, Mouse, Non-human primate
应用 :
Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 1:20-1:200, Immunoprecipitation: 2 µg/mL, Western Blot: 0.4 - 1 µg/mL
物种 :
Human, Mouse, Non-human primate
克隆 :
111
抗体亚型 :
IgG1, kappa
储存 :
4° C
描述 :
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
免疫原 :
Human B cell nuclei from plasmacytoid 2p68 cells
格式 :
液体
应用w/稀释 :
Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 1:20-1:200, Immunoprecipitation: 2 µg/mL, Western Blot: 0.4 - 1 µg/mL
别名 :
86 kDa subunit of Ku antigen; AI314015; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; FLJ39089; G22p2; HGNC:12833; KARP1; KARP-1; Ku autoantigen; ku autoantigen protein p86 homolog; Ku autoantigen, 80kD); Ku autoantigen, 80kDa; Ku p80; Ku80; Ku-80; Ku86; Ku-86; Ku86 autoantigen related protein 1; KUB2; Kup80; Lupu; lupus; lupus Ku autoantigen protein p86; NFIV; nuclear factor IV; OTTHUMP00000164061; OTTHUMP00000206791; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa); X-ray repair cross complementation (double-strand-break rejoining; X-ray repair cross complementation (double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross complementing 5; X-ray repair cross-complementing protein 5; Xrcc5
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国